ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

Early Alloantibody Responses Against Donor MHC Class I and Class II Molecules Are Differentially Affected by Prolonged Cold Ischemia Storage of Renal Allografts.

V. Gorbacheva, R. Fan, W. Baldwin, A. Valujskikh.

Immunology, Cleveland Clinic Foundation, Cleveland, OH.

Meeting: 2016 American Transplant Congress

Abstract number: 396

Keywords: IgG, Inflammation, Kidney transplantation, Mice

Session Information

Date: Tuesday, June 14, 2016

Session Name: Concurrent Session: Ischemic Injury and Organ Preservation: Animal Models

Session Time: 2:30pm-4:00pm

 Presentation Time: 3:30pm-3:42pm

Location: Room 313

Related Abstracts
  • Allograft Class II MHC and CD40 Expression Are Required for Endogenous Memory CD8 T Cell Proliferation in Cardiac Allografts Subjected to Prolonged Cold Ischemic Storage
  • Absence of Allograft TLR4 Receptor Signaling Attenuates Early Inflammation and Promotes Survival of Allografts Subjected to Prolonged Cold Ischemic Storage

Prolonged cold ischemia storage (CIS) of donor transplants is a major risk factor for acute and chronic graft tissue injury. Despite strong clinical correlations between donor specific alloantibody (DSA) reactivity and transplant outcome, the relationships between posttransplant inflammation and the development of DSA with different specificities have not been addressed. The goal of this study was to test how increased CIS time influences humoral alloimmunity in mouse model of renal transplantation.

B6 (H-2b) mice received BALB/c (H-2d) renal allografts subjected to 0.5 h or 6 h CIS in University of Wisconsin solution. Second recipient kidney was removed at the time of transplantation. Serum creatinine levels were <0.4 mg/dl in all recipients at d.60 posttransplant. The grafts from 0.5 h CIS group had moderate segmental sclerosis in a limited number of glomeruli and perivascular cell infiltrates. In contrast, 6 h CIS grafts had extensive glomerular injury including thickened capillary loops, segmental or global sclerosis and conspicuous thrombotic microangiopathy with red cell congestion, intracapillary fibrin thrombi, and mesangiolysis.

Recipient serum was analyzed for donor MHC class I (Dd) and class II (I-Ad) reactive IgG DSA by ELISA on d.14 posttransplant. Compared to 0.5 h CIS group, 6 h CIS recipients had significantly higher levels of DSA against donor class II (OD 0.56±0.08 vs 0.24±0.01 at 1:500 serum dilution) but not class I (OD 0.51±0.06 vs 0.43±0.11) MHC molecules. At 60 d. posttransplant, 6 h CIS recipients had higher frequency of spleen ASCs producing donor class I and class II reactive IgG DSA than 0.5 h CIS controls (12.4±2.3 vs 5±2.9 cells and 8.5±3.5 vs 2.7±0.9 cells per 10×106 splenocytes for Dd– and I-Ad-reactive ASCs, respectively). Notably, both groups had comparable frequencies of IFNγ producing donor-reactive T cells at 60 d. posttransplant suggesting that the augmented humoral rather than cellular immune responses account for the transplant glomerulopathy observed in 6 h CIS recipients.

Our data show that prolonged CIS enhances early DSA responses against donor MHC class II but not class I antigens and promotes the generation of donor class I and class II reactive long-lived ACSs. This is the first indication that posttransplant inflammation not only facilitates DSA development but also shapes the specificity of early DSA responses and may thus influence renal allograft pathology.

CITATION INFORMATION: Gorbacheva V, Fan R, Baldwin W, Valujskikh A. Early Alloantibody Responses Against Donor MHC Class I and Class II Molecules Are Differentially Affected by Prolonged Cold Ischemia Storage of Renal Allografts. Am J Transplant. 2016;16 (suppl 3).

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Gorbacheva V, Fan R, Baldwin W, Valujskikh A. Early Alloantibody Responses Against Donor MHC Class I and Class II Molecules Are Differentially Affected by Prolonged Cold Ischemia Storage of Renal Allografts. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/early-alloantibody-responses-against-donor-mhc-class-i-and-class-ii-molecules-are-differentially-affected-by-prolonged-cold-ischemia-storage-of-renal-allografts/. Accessed March 4, 2021.

« Back to 2016 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Penis Transplantation: First U.S. Experience.
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Penis Transplantation: First U.S. Experience.
  • A Decade of Donor-Derived Disease: A Report of the OPTN Ad Hoc Disease Transmission Advisory Committee (DTAC).
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Penis Transplantation: First U.S. Experience.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.