ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

Donation After Circulatory Death Kidneys With Prolonged Delayed Graft Function Present Widespread Metabolic And Translational Deficiencies At Time Of Retrieval

M. L. Lo Faro1, K. Rozenberg1, H. Huang1, S. Maslau1, H. Leuvenink2, E. Sharples3, R. Ploeg1

1Nuffield Department of Surgical Sciences, University of Oxford, Oxford, United Kingdom, 2University Medical Centre Groningen, Groningen, Netherlands, 3Oxford Transplant Centre, Oxford, United Kingdom

Meeting: 2019 American Transplant Congress

Abstract number: 601

Keywords: Kidney, Renal injury, Renal ischemia

Session Information

Date: Tuesday, June 4, 2019

Session Name: Concurrent Session: Ischemia Reperfusion & Organ Rehabilition III

Session Time: 4:30pm-6:00pm

 Presentation Time: 4:54pm-5:06pm

Location: Room 313

Related Abstracts
  • Organ Resilience Contributes to Different Impact of Delayed Graft Function (DGF) on Graft Survival in Kidneys Donated by Brain Death (DBD) and Circulatory Death Donors (DCD)
  • Association Between Delayed Graft Function and Long-Term Outcomes After Kidney Transplantation from Donors After Circulatory Death.

*Purpose: Delayed graft function (DGF) is acute renal failure post-transplant, commonly defined as the need for dialysis in the first week post-transplant. Injury at time of death affects graft quality in different ways. Donor, recipient, preservation factors and IRI all contribute to DGF, but there is currently no way of predicting DGF or its duration. This study investigated the biological pathways in DCD kidneys at time of donation that related to DGF and could discriminate different DGF durations.

*Methods: N=30 DCD kidney biopsies were selected from the UK Quality in Organ Donation (QUOD) biobank and stratified according to outcome and DGF duration (immediate function, IF n=10, short DGF (1-6 days), SDGF n=10; long DGF (7-22 days), LDGF n=10). Samples were matched for donor and recipient age, gender, BMI (<30), f-WIT, no donor AKI and CIT (≤ 18h). Proteins were extracted and analysed by LC-MS/MS proteomics. Pathway analysis was run by Ingenuity Pathway Analysis. Correlations between protein levels and DGF duration were studied by Pearson correlation.

*Results: 3,999 proteins were identified and n=418, n=181 and n=374 were significantly different (p<0.05, unpaired t-test) in SDGF vs IF, LDGF vs IF and LDGF vs SDGF respectively. SDGF kidneys presented activation of stress pathways geared towards cell survival (eIF2 and autophagy signalling) when compared to IF, while LDGF kidneys presented impaired response to stress (downregulation of Nrf2-mediated oxidative stress response). eIF2, mTOR signalling and glycolysis were all downregulated in LDGF vs SDGF (Figure 1). Histone H3.3, which accumulates at sites of DNA injury, was increased in LDGF and its levels correlated with DGF duration (Pearson r 0.7224).

*Conclusions: DCD kidneys with short duration of DGF present acute cellular injury at time of donation, alongside upregulation of repair pathways (e.g. chaperone-mediated autophagy, eIF2-dependent protein translation). In contrast, DCD kidneys with prolonged DGF present widespread translational, metabolic and antioxidant deficiencies. These pathways could be targeted therapeutically to reduce DGF incidence and duration.

 border=

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Faro MLLo, Rozenberg K, Huang H, Maslau S, Leuvenink H, Sharples E, Ploeg R. Donation After Circulatory Death Kidneys With Prolonged Delayed Graft Function Present Widespread Metabolic And Translational Deficiencies At Time Of Retrieval [abstract]. Am J Transplant. 2019; 19 (suppl 3). https://atcmeetingabstracts.com/abstract/donation-after-circulatory-death-kidneys-with-prolonged-delayed-graft-function-present-widespread-metabolic-and-translational-deficiencies-at-time-of-retrieval/. Accessed January 22, 2021.

« Back to 2019 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Penis Transplantation: First U.S. Experience.
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Live Related Kidney Transplant Experience in Abuja, Nigeria – First Eight Cases Ever.
    • Penis Transplantation: First U.S. Experience.
    • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
    • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
    • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
    • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes

    Visit Our Partner Sites

    American Transplant Congress (ATC)

    Visit the official site for the American Transplant Congress »

    American Journal of Transplantation

    The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

    American Society of Transplantation (AST)

    An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

    American Society of Transplant Surgeons (ASTS)

    The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

    Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

    Privacy Policy

    loading Cancel
    Post was not sent - check your email addresses!
    Email check failed, please try again
    Sorry, your blog cannot share posts by email.
    This site uses cookies: Find out more.