ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

Development of a Sequencing-Based Cytomegalovirus UL55 Glycoprotein B (gB) Genotyping Assay.

S. Cowden, K. Grantham, I. Caton, J. Hester, M. Altrich, S. Kleiboeker.

Viracor-IBT Laboratories, Lee's Summit, MO.

Meeting: 2016 American Transplant Congress

Abstract number: C288

Keywords: Cytomeglovirus, Genomics

Session Information

Date: Monday, June 13, 2016

Session Name: Poster Session C: Viruses and SOT

Session Time: 6:00pm-7:00pm

 Presentation Time: 6:00pm-7:00pm

Location: Halls C&D

Related Abstracts
  • Highly Accurate High-Resolution HLA Imputation Using Dense Genotyping Data
  • VDJ Immune Repertoire Sequencing Predicts Patients at Risk of Alloimmune Injury.

Background: Human cytomegalovirus (hCMV) is an important agent of serious viral infection in the solid organ transplant and allogeneic hematopoietic stem cell transplant populations. Severe hCMV infections are associated with graft rejection and other comorbidities. The hCMV gB is a major envelope glycoprotein and is associated with host cell entry and cell-to-cell viral transmission. The gB genotypes (gB1-gB4) have been shown to have varied cytopathic effects on culture cell lines influencing pathogenicity and severity of CMV-associated disease. Four distinct hCMV genotypes have been described based on sequence variations within a region of the UL55 gene using PCR and restriction analysis. Here we describe a sequencing-based method for determining the gB genotype of clinical hCMV isolates.

Methods: End-point PCR with hCMV specific primers to amplify a portion of the UL55 gene in CMV-positive plasma samples followed by dideoxy-terminator sequencing to collect bidirectional nucleotide sequences between codons 337 and 525 was used. Sequence data collected were aligned with gB genotype-specific consensus sequences constructed from type-strains and sequences published in peer-reviewed journals. Phylogenetic analysis was performed by the Tamura-Nei genetic distance model using the neighbor-joining method with 1,000 bootstrap replicates. The resulting dendrogram and percent identity matrix were used to assign gB genotypes to the samples tested.

Results: The gB genotype distribution in 67 clinical hCMV samples was found to be: 29 gB1 (43.3%), 10 gB2 (14.9%), 15 gB3 (22.4%), 8 gB3 variant (11.9%), 4 gB4 (6.0%), and 1 mixed infection containing gB1 and gB4 genotypes (1.5%). All samples shared ≥98% identity with their respective gB consensus sequence with the exception of 1 gB3 sample (96.3% identity to gB3 consensus) and the mixed sample (gB1 and gB4 identities 95.4% and 95.6%, respectively).

Conclusions: The prognostic benefits associated with confirming the gB genotype in CMV-positive patients undergoing immunosuppressive therapy is currently unclear but recent reports have shown the potential importance of this information. We describe here an accurate and sensitive means to detect and determine hCMV gB genotype(s) in clinical plasma specimens.

CITATION INFORMATION: Cowden S, Grantham K, Caton I, Hester J, Altrich M, Kleiboeker S. Development of a Sequencing-Based Cytomegalovirus UL55 Glycoprotein B (gB) Genotyping Assay. Am J Transplant. 2016;16 (suppl 3).

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Cowden S, Grantham K, Caton I, Hester J, Altrich M, Kleiboeker S. Development of a Sequencing-Based Cytomegalovirus UL55 Glycoprotein B (gB) Genotyping Assay. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/development-of-a-sequencing-based-cytomegalovirus-ul55-glycoprotein-b-gb-genotyping-assay/. Accessed March 3, 2021.

« Back to 2016 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Penis Transplantation: First U.S. Experience.
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Penis Transplantation: First U.S. Experience.
  • A Decade of Donor-Derived Disease: A Report of the OPTN Ad Hoc Disease Transmission Advisory Committee (DTAC).
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Penis Transplantation: First U.S. Experience.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.