Date: Monday, May 4, 2015
Session Time: 5:30pm-6:30pm
Presentation Time: 5:30pm-6:30pm
Location: Exhibit Hall E
Background: Microvascular damage has become the real protagonist involved in antibody-mediated rejection and NK-cell antibody-dependent cell mediated cytotoxicity (ADCC) has been proposed as a contributing factor. We have assessed the potential relationship between circulating HLA antibodies and different NK cellular subsets in kidney transplant (KT) recipients.
Methods: Observational exploratory study including 393 KT recipients: 38 with DSA, 55 with HLA non-DSA and 300 without HLA antibodies in a data base collecting demographics and clinical data. Contemporaneous analysis of HLA antibodies (Luminex® screening and single-antigen tests) and peripheral NK immunophenotype long-term after grafting.
Results: Multivariate analysis indicated that retransplantation (OR: 5.37 (2.18-13.25), p 0.000), pretransplant sensitization (OR:1.1 (1.01-1.05),p0.08) and the percentage of NKG2A cells (1.03 (1.01-1.05), p=0.002) were associated with detection of post-KT DSA compared with patients without antibodies. Recipient female gender (OR: 3.69 (1.19-11.36), p 0.02), DR mismatch (OR: 5.97 (2.14-16.6), p 0.001), acute rejection (OR: 16.51 (1.42-190.83), p 0.025), and the percentage of NKG2A cells (OR: 1.04 (1.007-1.076), p 0.018) were significantly associated with post-KT DSA compared to HLA non-DSA. In contrast with patients without detectable anti-HLA antibodies, DSA and HLA non-DSA patients displayed lower proportions of NK-cells, associated with increased CD56bright and NKG2A subsets, the latter being more marked in DSA cases.
Conclusions: Our results suggest that the assessment of the NK-cell immunophenotype may contribute to define signatures of alloreactive humoral responses in renal allograft recipients.
To cite this abstract in AMA style:Crespo M, Redondo D, Yelamos J, Muntasell A, Pérez-Sáez M, Lopez-Montañes M, Garcia C, Torío A, Hernandez J, Mir M, Lopez-Botet M, Pascual J. Detection of Donor-Specific Anti-HLA Antibodies Is Associated With Changes in the Peripheral Distribution of NK Cell Subsets in Kidney Allograft Recipients [abstract]. Am J Transplant. 2015; 15 (suppl 3). https://atcmeetingabstracts.com/abstract/detection-of-donor-specific-anti-hla-antibodies-is-associated-with-changes-in-the-peripheral-distribution-of-nk-cell-subsets-in-kidney-allograft-recipients/. Accessed January 20, 2021.
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