Characterization of Peripheral Blood T and B Cells of Sensitized Kidney Transplant Recipients With Long Term Stable Graft Function

N. Galante,¹ M. Fernandes,¹ F. Agena,¹ A. Triboni,¹ G. Freitas,¹ A. Aguirre,¹ W. Nahas,¹ V. Coelho,² E. David-Neto.¹

¹Renal Transplant Service, Hospital das Clínicas - University of Sao Paulo School of Medicine, Sao Paulo, Brazil
²Laboratory of Immunology, Heart Institute - University of Sao Paulo School of Medicine, Sao Paulo, Brazil.

Meeting: 2015 American Transplant Congress

Abstract number: D31

Keywords: Immunosuppression, Kidney transplantation, Lymphocytes, Sensitization

Session Information

Session Name: Poster Session D: Costimulation and Signaling in Lymphocytes

Session Type: Poster Session

Date: Tuesday, May 5, 2015

Session Time: 5:30pm-6:30pm

Presentation Time: 5:30pm-6:30pm

Location: Exhibit Hall E

We examined the phenotype of T and B cells of peripheral blood of sensitized kidney transplant recipients with stable graft function (GFR > 29mL/min) enrolled in a prospective randomized controlled trial of late conversion from tacrolimus (TAC)/sodium mycophenolate (MPS)/and prednisone (PRED) immunosuppression to TAC/MPS/PRED and sirolimus. The frequencies and absolute numbers of T CD4+ naïve (CD3+CD8+CD45RA+CCR7+), T CD4+ central memory (CM) (CD3+CD4+CD45RA-CCR7-), T CD4+ effector memory (EM) (CD3+CD4+CD45RA-CCR7-), T CD8+ effector memory RA+ (TEMRA) (CD3+CD4+CD45RA+CCR7-), T Tregs (CD3+CD4+CD25+CD127lowFOXP3+), B naïve (CD19+CD24lowCD38low) B memory (CD19+CD24lowCD38-) and Bregs (CD19+CD24highCD38high) were determined using flow cytometry. Patients (n=10) were all female, aged 43.5 ± 10.5 years, Caucasian (n=8) and recipients of the first kidney transplant (n=9) from deceased donors (n=6). Most patients (n=7) have a PRA ranged 20-70%, history of pregnancy (n=10) and blood transfusions (n=6) before transplantation. The time after transplantation at enrollment was 4 ± 1.6 years. The percentages of T CD4+ naïve, CM, EM, T CD8+ TEMRA, T regs, B naïve, B memory and B regs were 6.6, 3.6 and 64.9% of gated CD3+ CD4+ cells, 68.2% of gated CD3+ CD8+ cells, 26,4% of gated CD3+ CD4+ CD25+ cells and 8.8, 23.5 and 8.8% of gated CD19+ cells respectively, and the absolute numbers were, 31, 15, 234, 93, 7, 26, 23 and 5 cells / mm3 of blood respectively. Sensitized kidney transplant recipients with stable graft function later after transplantation exhibit predominance of memory phenotype in both T and B lymphocytes subsets.

To cite this abstract in AMA style:

Galante N, Fernandes M, Agena F, Triboni A, Freitas G, Aguirre A, Nahas W, Coelho V, David-Neto E. Characterization of Peripheral Blood T and B Cells of Sensitized Kidney Transplant Recipients With Long Term Stable Graft Function [abstract]. Am J Transplant. 2015; 15 (suppl 3). https://atcmeetingabstracts.com/abstract/characterization-of-peripheral-blood-t-and-b-cells-of-sensitized-kidney-transplant-recipients-with-long-term-stable-graft-function/. Accessed November 21, 2024.

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