Date: Saturday, June 2, 2018
Session Time: 5:30pm-7:30pm
Presentation Time: 5:30pm-7:30pm
Location: Hall 4EF
Exosomes are small vesicles which contains biological molecules including proteins, RNA`s and miRNAs. It has been reported that exosomes may contain ds DNA originated from different types of malignant cells. In this report, we determined the presence of ds DNA in exosomes from lung transplant recipients (LTxRs). We isolated DNA from LTxRs who are stable, acute rejection, chronic rejection (Bronchiolitis Obliterans Syndrome (BOS)) and respiratory viral infections. Further analysis and sequencing of ds DNA from exosomes identified the presence of staggered DNA sequences and 126 are common in all the 4 samples. After different sets of analysis and comparing all the 4 data sets highest diversity of sequences was observed in samples analyzed from BOS LTxRs with 62 unique sequences, i.e (CFTRP3, CNTNAP2, MIR6724-3, USP17L17, CR848007.2, TOP1MT etc.), 3 unique sequences in viral,i.e (RP11-593P24.4, RP11-302L19.1, BSPH1), 14 in stable, i.e (CDK5RAP2, RP11-19N8.2, PIP5K1P2, SNORD44, RNA5-8SP2, AC124853.1, CLPTM1L, USP17L22, MIR8078, ADAP1) and 11 in acute rejection, i.e (RNA5-8S5, AL732375.7, PTPRN2, RP11-374M1.11, AC126281.1, SNX27, RN7SL754P, RAB11FIP3, OPRL1, AC016549.1, CTD-2228K2.1). All unique sequences were detected in different sets of samples and are associated with diverse regulatory pathways. In comparison to stable LTxRs we identified 29 unique sequences in acute, 85 in BOS and 15 in viral LTxRs exosomes (listed below in Table). In conclusion, we demonstrate the presence of unique ds DNA sequences with in the exosomes isolated from LTxRs with rejection and viral infections suggesting that these ds DNA will have functional consequences.
|Samples||Unique Sequences and Related Pathways V/s Stable|
|Acute Rejection||VxPx cargo-targeting to cilium, DCC mediated attractive signaling, Caspase activation via extrensic apoptotic signaling pathway, Cargo trafficking to the periciliary membrane|
|BOS||Activation of NMDA receptor upon glutamate binding and postsynaptic events, Activatio nof t he mRNA upon binding of the cap-binding complex and eIFs, ARMS-mediated activation, AURKA activation by TPX2, Axonal growth inhibition (RHOA activation)|
|Viral Infections||DCC mediated attractive signaling, Defensins|
CITATION INFORMATION: Bansal S., Sharma M., Walia R., Bremner R., Smith M., Mohanakumar T. Characterization of Exosomal DNA from Human Lung Transplant Recipients Am J Transplant. 2017;17 (suppl 3).
To cite this abstract in AMA style:Bansal S, Sharma M, Walia R, Bremner R, Smith M, Mohanakumar T. Characterization of Exosomal DNA from Human Lung Transplant Recipients [abstract]. https://atcmeetingabstracts.com/abstract/characterization-of-exosomal-dna-from-human-lung-transplant-recipients/. Accessed November 15, 2019.
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