Date: Saturday, May 30, 2020
Session Time: 3:15pm-4:00pm
Presentation Time: 3:30pm-4:00pm
*Purpose: Despite the technologically advanced integrated systems of continuous glycemic control and insulin supply, it is still impossible to obtain results of diabetes treatment in comparison with normal pancreas function. Currently, the only way to achieve a glycemic control comparable to a healthy person in a diabetic person is by pancreas organ transplantation. The limitations of this method related to the inadequate access to organs, potential complications associated with the procedure and the loads resulting from immunosuppressive treatment mean that solutions are sought that would give a chance to be used on a large patient population and obtain glycemic control results as in healthy people. One of the solutions considered is the use of 3D printing technology using xenogenic material and stem cells. The aim of the study was to asses biointegrity and functionality of printed pancreatic petals implanted in mice.
*Methods: As part of the work, two experiments were carried out: (I) SCID mice in which type I diabetes was induced using STZ, where implanted printed constructs from biological material containing isolated porcine islets were implanted, (II) on a B6 mice into which printed constructs without pancreatic islets were implanted to assess the body’s immune response. The results were analyzed using histological, immunohistochemical and biochemical tests.
*Results: Based on the results obtained, it was demonstrated that biochemical evaluation showed an increase in C-peptide concentration and a decrease in glucose in SCID mice with pre-induced diabetes. The implanted constructs do not elicit an immune response in B6 mice.
*Conclusions: The possibility of using printed constructs as bio-integrated and functional implants has been demonstrated.
To cite this abstract in AMA style:Wszola M, Klak M, Tymicki G, Berman A, Kosowska K, Bryniarski T, Cywoniuk P, Gomolka M, Kowalska P, Turowski P, Kaminski A. Biointegration and Function of 3D Printed Bionic Pancreatic Petals on a Mouse Model [abstract]. Am J Transplant. 2020; 20 (suppl 3). https://atcmeetingabstracts.com/abstract/biointegration-and-function-of-3d-printed-bionic-pancreatic-petals-on-a-mouse-model/. Accessed March 6, 2021.
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