ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

B Cell Depletion with Anti-CD20 mAb Exacerbates Anti-Donor CD4+ T Cell Responses in Highly Sensitized Transplant Model

A. Tanaka, K. Ide, Y. Tanaka, M. Ohira, H. Tahara, H. Morimoto, H. Ohdan

Gastroenterological and Transplant Surgery, Hiroshima University, Hiroshima, Japan

Meeting: 2020 American Transplant Congress

Abstract number: LB-048

Keywords: Alloantibodies, CD20, Immunosuppression, T cell activation

Session Information

Date: Saturday, May 30, 2020

Session Name: Poster Session D: Late Breaking

Session Time: 3:15pm-4:00pm

 Presentation Time: 3:30pm-4:00pm

Location: Virtual

Related Abstracts
  • B-Cell Depletion with Rituximab Exacerbates Anti-Donor CD4+ T-Cell Responses in Patients with Donor-Specific Anti-Human Leukocyte Antigen Antibodies
  • Transient Cd4+ T Cell and Cd20+ B Cell Depletion Leads to Prolonged Pig-To-Primate Kidney Xenograft Survival

*Purpose: Preformed donor-specific anti-human leukocyte antigen antibodies (DSAs) have been associated with detrimental effects in transplant recipients. To eliminate preformed DSAs, we desensitized DSA-positive recipients by injecting anti-CD20 monoclonal antibodies (mAbs) and then performed plasmapheresis using a protocol for ABO-incompatible (ABO-I) transplant recipients. However, in contrast to ABO-I transplant recipients who did not affect anti-donor CD4+T-cell responses, hyper responses of anti-donor CD4+T-cell were observed in DSA-positive recipients after desensitization therapy. In this study, we investigated the effect of anti-CD20 mAb-mediated B-cell depletion on anti-donor T-cell responses and the inhibitory role of B-cells in a highly sensitized transplant model.

*Methods: Naïve Balb/c mice were transplanted with C57BL/6 skin grafts (twice; day 0, 14) to prepare a highly sensitized model. On day 42, sensitized Balb/c mice were injected with anti-CD20 mAb to eliminate B-cells (desensitization group). Seven days after the injection (day49), a mixed lymphocyte reaction assay using a carboxyfluorescein succinimidyl ester-labeling technique (CFSE-MLR) were performed to evaluate the effect of B-cell depletion on anti-donor T-cell responses. The control group was administered with isotype-matched control antibodies. Furthemore, to investigate the inhibitory effects of B-cells, CD19+whole B-cells, B1(CD5+CD19+) cells and non B1(CD5–CD19+) cells were co-cultured in CFSE-MLR assay of desensitization group respectively, and the effect of various B-cell subsets on anti-donor T-cell responses were evaluated.

*Results: The average stimulation index (SI) values for the CD4+T-cell responses to donor stimulation were significantly higher in the desensitization group ​​than those in the control group. When co-cultured with various B-cells in CFSE-MLR assay of the desensitization group, the addition of whole CD19+B-cells and B1-cells significantly suppressed the anti-donor CD4+T-cell responses. However, the addition of non B1-cells did not suppress the anti-donor CD4+T-cell responses.

*Conclusions: These findings demonstrate that B-cell depletion with anti-CD20 mAb exacerbates anti-donor CD4+T-cell responses in a highly sensitized transplant model. It might be possible that the sensitized CD5+ CD19+B1-cells have an ability to inhibit anti-donor CD4+T-cell responses.

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Tanaka A, Ide K, Tanaka Y, Ohira M, Tahara H, Morimoto H, Ohdan H. B Cell Depletion with Anti-CD20 mAb Exacerbates Anti-Donor CD4+ T Cell Responses in Highly Sensitized Transplant Model [abstract]. Am J Transplant. 2020; 20 (suppl 3). https://atcmeetingabstracts.com/abstract/b-cell-depletion-with-anti-cd20-mab-exacerbates-anti-donor-cd4-t-cell-responses-in-highly-sensitized-transplant-model/. Accessed March 6, 2021.

« Back to 2020 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • Role of Mitochondria, Metabolism and Inflammation: Pathways Influencing Short Term Outcomes Post-Renal Transplantation
    • Factors Influencing Creatinine Rise Following Donor Nephrectomy

    Visit Our Partner Sites

    American Transplant Congress (ATC)

    Visit the official site for the American Transplant Congress »

    American Journal of Transplantation

    The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

    American Society of Transplantation (AST)

    An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

    American Society of Transplant Surgeons (ASTS)

    The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

    Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

    Privacy Policy

    loading Cancel
    Post was not sent - check your email addresses!
    Email check failed, please try again
    Sorry, your blog cannot share posts by email.
    This site uses cookies: Find out more.