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Anti-Fibrotic Property of Adipose Derived Regenerative Cells on Hydrogen Peroxide Induced Injury in Nrk-52e Cells

R. Chawangwongsanukun1, R. Lathan1, R. Pearson2, M. Clancy2, P. Mark1

1Institute of Cardiovascular and Medical Sciences, Glasgow, United Kingdom, 2Transplant Unit, Queen Elizabeth University Hospital, Glasgow, United Kingdom

Meeting: 2022 American Transplant Congress

Abstract number: 1310

Keywords: Fibrosis, Inflammation, Renal injury, Stem cells

Topic: Basic Science » Basic Science » 16 - Biomarkers: -omics and Systems Biology

Session Information

Session Name: Biomarkers: -omics and Systems Biology

Session Type: Poster Abstract

Date: Monday, June 6, 2022

Session Time: 7:00pm-8:00pm

 Presentation Time: 7:00pm-8:00pm

Location: Hynes Halls C & D

*Purpose: Adipose derived regenerative cells (ADRCs) are a heterogenous cell population derived from adipose tissue. These cells are composed of endothelial progenitor cells, pericytes, and adipose stromal cells. Previous studies showed that ADRCs have reparative effects through anti-inflammatory, and anti-fibrotic activity, and can potentially serve for the rehabilitation of solid organ transplants. We used hydrogen peroxide to induce injury in a rat proximal tubular cell line, NRK-52E cells, mimicking oxidative stress and damage of a transplant kidney. Our study investigates fibrosis-related mechanisms of ADRC renal repair.

*Methods: NRK-52E cells were assigned to four groups including non-injury, injury, ADRC-treated non-injury, and ADRC-treated injury groups. The cells were induced injury by incubation with a 500 µM hydrogen peroxide for 4 hours. ADRCs were added (10% ratio of total NRK-52E cells) and expression of target genes was assayed by real-time polymerase chain reaction (RT-qPCR) after 24 hours of ADRC treatment.

*Results: The ADRC-treated injured group had significantly decreased collagen type I (COL1) mRNA expression compared to non-injury group (p=0.0439) (figure 1) and had a significantly decreased neutrophil gelatinase-associated lipocalin (NGAL) mRNA expression compared to injury group (p=0.0015) (figure 2).

*Conclusions: This study showed that ADRCs have positive response by reducing kidney injury markers and by decreasing progenitor fibrotic factors of NRK-52E cells and implies ADRCs therapeutic remodelling of the extracellular matrix.

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To cite this abstract in AMA style:

Chawangwongsanukun R, Lathan R, Pearson R, Clancy M, Mark P. Anti-Fibrotic Property of Adipose Derived Regenerative Cells on Hydrogen Peroxide Induced Injury in Nrk-52e Cells [abstract]. Am J Transplant. 2022; 22 (suppl 3). https://atcmeetingabstracts.com/abstract/anti-fibrotic-property-of-adipose-derived-regenerative-cells-on-hydrogen-peroxide-induced-injury-in-nrk-52e-cells/. Accessed May 9, 2025.

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