Acquired Down-Regulation of B Cell Function Induced by Donor Blood Group Antigens After ABO-Incompatible Kidney Transplantation.
1Division of Urology, Department of Regenerative & Transplant Medicine, Niigata Graduate School of Medical and Dental Sciences, Niigata, Japan
2Division of Clinical Nephrology and Rheumatology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan
3Division of Transfusion Medicine and Regenerative Medicine Bioscience Medical Center, Niigata University Medical and Dental Hospital, Niigata, Japan.
Meeting: 2016 American Transplant Congress
Abstract number: D90
Keywords: Antibodies, B cells, Kidney transplantation, Tolerance
Session Information
Date: Tuesday, June 14, 2016
Session Name: Poster Session D: Clinical Science: Tolerance: Clinical Studies
Session Time: 6:00pm-7:00pm
Presentation Time: 6:00pm-7:00pm
Location: Halls C&D
Background: The long-term of B cell immunobiology against donor blood group antigen has not been elucidated in ABO incompatible kidney transplantation (ABOi KTx).
Methods: The antibody titers against donor blood group antigen were serially measured by hemagglutination technique in 50 out of 82 patients who underwent ABOi KTx (Mean follow-up were 72 months). We stimulated peripheral blood mononuclear cells in vitro to investigate if B cells can produce antibodies against donor blood group antigens in the absence of graft adsorption in vivo. 5 [times] 105 cells per well were cultured with 2.5% human serum of blood type AB, CpG oligodeoxynucleotide 2006 (ODN), recombinant human (rh) IL-2, rhIL-10, and rhIL-15 for 7 days. Antibodies against A or B blood group sugar antigens in culture supernatant were measured by specific enzyme-linked immunodorbent assay. 35 healthy volunteers and 57 patients who underwent ABO compatible KTx were compared as control.
Results: Serum donor specific antibody titer (both of IgM and IgG) were maintained very low (lower than 1:4) in 42 patients (84%). However, the antibody against non-donor blood type antigen was continuously produced in patients whose blood type were O. Antibody productions in culture supernatant were not detected specifically to donor blood group antigens in ABOi KTx patients while antibodies were clearly observed in control groups. Graft kidney biopsies were performed in 8 patients after ABOi KTx. None of the patients had IgM or IgG depositions on the endothelial cells. One patient whose blood type was A received 2nd ABOi KTx after 1st graft function was lost. Both donors were blood type B. Even though he had second antigen exposure, donor specific antibody (anti-B antibody) was not produced at all.
Conclusion: These findings suggests that intentional exposure to non-self A and B antigens contributes to B cell down-regulation in ABOi KTx.
CITATION INFORMATION: Tasaki M, Saito K, Nakagawa Y, Imai N, Aoki T, Kamimura M, Takahashi K, Tomita Y. Acquired Down-Regulation of B Cell Function Induced by Donor Blood Group Antigens After ABO-Incompatible Kidney Transplantation. Am J Transplant. 2016;16 (suppl 3).
To cite this abstract in AMA style:
Tasaki M, Saito K, Nakagawa Y, Imai N, Aoki T, Kamimura M, Takahashi K, Tomita Y. Acquired Down-Regulation of B Cell Function Induced by Donor Blood Group Antigens After ABO-Incompatible Kidney Transplantation. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/acquired-down-regulation-of-b-cell-function-induced-by-donor-blood-group-antigens-after-abo-incompatible-kidney-transplantation/. Accessed March 3, 2021.« Back to 2016 American Transplant Congress