Date: Sunday, May 3, 2015
Session Time: 5:30pm-6:30pm
Presentation Time: 5:30pm-6:30pm
Location: Exhibit Hall E
Aim: Allele-specific HLA antibodies are commonly detected by Luminex single antigen bead assays in solid organ transplant recipients. However, high-resolution HLA typing for deceased donors is usually unavailable. This causes uncertainty in reporting donor-specific antibodies and virtual crossmatching. Therefore, we investigated the ability of haplotype analysis to predict high-resolution HLA-A, -B, -C, -DRB1 and -DQB1 and low-resolution HLA-DQA1 typing from low-resolution HLA-A, -B, -C, -DRB1 and -DQB1 typing and its possible application for virtual crossmatching.
Methods: Haplotypes (n=270) from proficiency testing samples (n=135), with known low- and high-resolution typing results, were analyzed based on data compiled from the NMDP registry database (Maiers M, et al. Human Immunology 2007; 68: 779) and the ethnic background of the sample. Low-resolution HLA typing (-A, -B, -C, -DRB1 and -DQB1) was entered in a haplotype analysis tool and the predicted high-resolution typing was compared to the actual results. Subsequently, using the high-resolution HLA-DRB1 and HLA-DQB1 typing, we predicted the low-resolution HLA-DQA1 typing based on common HLA-DRB1, -DQA1 and -DQB1 allele associations (Fernandez-Viña M, et al. Immunogenetics 1991; 34: 299) and compared to the actual results.
Results: The haplotype analysis tool correctly predicted high-resolution HLA typing 87% of the time with the highest prediction rate for HLA-C (90%) and HLA-DQB1 (90%) and the lowest for HLA-DRB1 (82%) (Table 1). In addition, the predicted typing rate for HLA-DQA1 was 95% (Table 1).
Conclusion: Haplotype analysis based on common allele associations is an accurate method to predict high-resolution HLA-A, -B, -C, -DRB1 and -DQB1 typing and low-resolution HLA-DQA1 typing. This method allows for identification of allele-specific donor-specific antibodies with greater accuracy and further aids in solid organ allocation as well as post-transplant management of sensitized patients.
|HLA Locus||HLA Typing Prediction Rate||Predicted HLA Typing Resolution|
To cite this abstract in AMA style:Jaramillo A, Vaidya R, Pando M, Gaitonde S. Accurate Prediction of High-Resolution HLA-A, -B, -C, -DRB1 and -DQB1 Typing and Low-Resolution HLA-DQA1 Typing by Haplotype Analysis [abstract]. Am J Transplant. 2015; 15 (suppl 3). https://atcmeetingabstracts.com/abstract/accurate-prediction-of-high-resolution-hla-a-b-c-drb1-and-dqb1-typing-and-low-resolution-hla-dqa1-typing-by-haplotype-analysis/. Accessed June 23, 2021.
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