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A Mouse Model of Pneumonitis Induced by Candida Albicans Infection After Allogeneic Skin Transplantation

A. Yang, Z. Guo, W. Ju, Y. Ma, A. Hu, Y. Chen, M. Chen, C. Wu, X. He.

Organ Transplantation Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.

Meeting: 2015 American Transplant Congress

Abstract number: D109

Keywords: Fungal infection, Methodology, Mice, Skin transplantation

Session Information

Session Name: Poster Session D: Innate Immunity in Transplantation

Session Type: Poster Session

Date: Tuesday, May 5, 2015

Session Time: 5:30pm-6:30pm

 Presentation Time: 5:30pm-6:30pm

Location: Exhibit Hall E

Aims:Our study was to build up a mouse model of pneumonitis induced by candida albicans infection after allogeneic skin transplantation.

Methods:We used 10 male C57BL/6J mice as the donors, and 60 male Balb/c mice as the recipients. All mice were 6∼8 weeks old. After allogeneic skin transplantation model was built, cyclosporine A was injected into each recipient at a dose of 30 mg/kg/day throughout the study. All recipients were randomized into three groups at 7d post-operation, and received 200 ¯o;l candida albicans suspension of three different concentrations (5×103 CFU/ml, 5×104 CFU/ml and 5×105 CFU/ml) by mean of intranasal administration respectively. Mice were sacrificed at 1d, 3d, 7d and 14d post-infection, and the tissue of lung and kidney were collected for PCR analysis, tissue culture and histological evaluation.

Results:The DNA of candida albicans could be detected in the lung and kidney tissue of all groups with a range of 101∼103 copies. Many candida albicans colonies were grew in the lung tissue culture of the highest concentration group with a result of 368.96CFU/g at 1d, 3888.10CFU/g at 3d, 15.00CFU/g at 7d and 24.33CFU/g at 14d post-infection. Small counts of colonies were cultured with the kidney tissue in this group(0.00CFU/g at 1d, 15.00CFU/g at 3d, 2.50CFU/g at 7d and 1.50CFU/g at 14d post-infection). However, no colony of candida albicans was developed in the rest groups. After hematoxylin-eosin staining, the lung tissue of all groups showed various degrees of tissue edema and inflammatory cell infiltration. Methenamine silver staining only identified a small amount of candida albicans hypha in the lung tissue of the 5×105 CFU/ml group.

Conclusions:We built up a mouse model of pneumonitis induced by candida albicans infection after allogeneic skin transplantation successfully, with intranasal inoculation of 200¯o;l candida albicans suspension at the concentration of 5×105CFU/ml. Our model could be further used for investigations in the field of early diagnosis, treatment and prevention of fungal pneumonia in post-transplanted patients.

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To cite this abstract in AMA style:

Yang A, Guo Z, Ju W, Ma Y, Hu A, Chen Y, Chen M, Wu C, He X. A Mouse Model of Pneumonitis Induced by Candida Albicans Infection After Allogeneic Skin Transplantation [abstract]. Am J Transplant. 2015; 15 (suppl 3). https://atcmeetingabstracts.com/abstract/a-mouse-model-of-pneumonitis-induced-by-candida-albicans-infection-after-allogeneic-skin-transplantation/. Accessed May 12, 2025.

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