ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

α -1,3-galactosyltransferase Knockout Porcine Tissue And Organ May Reduce The Risks Of Antibody Mediated Rejection Via Less Rhesus Igm Cross-reactivity.

J. Yoon, B. Ezekian, M. Manook, J. Kwun, S. Knechtle

Duke Transplant Center, Durham, NC

Meeting: 2019 American Transplant Congress

Abstract number: D82

Keywords: Alloantibodies, Flowcytometry crossmatching, Sensitization, Xenoreactive antibodies

Session Information

Date: Tuesday, June 4, 2019

Session Name: Poster Session D: Xenotransplantation

Session Time: 6:00pm-7:00pm

 Presentation Time: 6:00pm-7:00pm

Location: Hall C & D

Related Abstracts
  • Newly-Revealed Antibody Targets Lead to the Early Loss of Triple Knockout Pig Kidneys in a Pig to Baboon Model
  • Rhesus Monkey Have natural Antibody toward Swine That is Not Influenced by Allosensitization nor by Carfilzomib/tocilizumab

*Purpose: Previously, we observed that sera from rhesus macaques contained preformed antibodies against wild type (WT) swine splenocytes. In the context of xenotransplantation, preformed xenoantibodies can elicit rapid antibody-mediated rejection (AMR). Galactose-α1,3-galactose (Gal), which is made by a-1,3-galactosyltransferase (GalT), is one of the major epitopes to which rhesus and human natural antibodies bind. Studies have shown less human antibody binding to GalT knockout (GalT-KO) pig cells compared to WT pig cells. In this study, we evaluated whether GalT deficiency of target cells would reduce binding of xeno-reactive antibody derived from allo-sensitized rhesus monkeys.

*Methods: In our sensitization model, pairs of rhesus macaques receive two subsequent skin transplants from each other to induce allosensitization. We used the rhesus serum samples from the pre-sensitization time point (PreS) and post-sensitization time point (PostS), and we measured xenoreactive antibodies with T-and B-cell flow crossmatches against WT and GalT-KO splenocytes. As negative controls (NC), splenocytes were also prepared for flow crossmatch without adding any serum.

*Results: Compared to the NC, PreS showed increased antibodies against both WT and GalT-KO (Figure 1). While there is no significant increase in IgG against WT after sensitization (PreS vs PostS), IgM levels against WT increased significantly after sensitization. On the other hand, the levels of IgG and IgM against GalT-KO showed no difference after sensitization.

*Conclusions: Rhesus sera contained pre-formed antibodies against GalT-KO, which implies a humoral immune barrier to xenotransplantation between rhesus and swine. We show that preformed rhesus antibodies against swine formerly observed with WT swine splenocytes were not just Gal-specific, but reactive against other undefined xenoantigens. Allosensitization increases IgM against WT significantly, but not against GalT-KO. This implies that compared to WT organs, xenotransplantation of GalT-KO organs to primates is associated with less risk of AMR in highly allosensitized recipients.

 border=

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Yoon J, Ezekian B, Manook M, Kwun J, Knechtle S. α -1,3-galactosyltransferase Knockout Porcine Tissue And Organ May Reduce The Risks Of Antibody Mediated Rejection Via Less Rhesus Igm Cross-reactivity. [abstract]. Am J Transplant. 2019; 19 (suppl 3). https://atcmeetingabstracts.com/abstract/%ce%b1-13-galactosyltransferase-knockout-porcine-tissue-and-organ-may-reduce-the-risks-of-antibody-mediated-rejection-via-less-rhesus-igm-cross-reactivity/. Accessed March 4, 2021.

« Back to 2019 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Penis Transplantation: First U.S. Experience.
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Penis Transplantation: First U.S. Experience.
  • A Decade of Donor-Derived Disease: A Report of the OPTN Ad Hoc Disease Transmission Advisory Committee (DTAC).
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Penis Transplantation: First U.S. Experience.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Evidence of a Clinically Significant Drug-Drug Interaction between Cannabidiol and Tacrolimus: A Case Report
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.