Articles tagged "Xenoreactive antibodies"

2020 American Transplant Congress
Transient Cd4+ T Cell and Cd20+ B Cell Depletion Leads to Prolonged Pig-To-Primate Kidney Xenograft Survival
B. P. Lovasik¹, A. J. Matar¹, D. A. Faber¹, D. V. Mathews¹, C. Breeden¹, S. C. Kim¹, A. J. Tector², A. B. Adams¹
¹Emory University School of Medicine, Atlanta, GA, ²University of Miami, Miami, FL
*Purpose: “Delayed” antibody-mediated xenograft rejection is the one of the most important obstacle to clinical application of pig organ xenografts. The aim of this study…
2020 American Transplant Congress
Overcoming Ab-Mediated Xenolung Rejection: Beta4Gal is an Important Pre- and Post-Transplant Ab Target, Relevant to Xenograft Injury in Baboons
L. Burdorf¹, Z. Abady¹, B. Cerel¹, K. Petitpas¹, C. Laird², S. Pratts¹, X. Cheng², C. J. Phelps³, W. Eyestone³, D. L. Ayares³, R. N. Pierson III¹, A. M. Azimzadeh¹
¹Surgery, Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, ²Surgery, University of Maryland, Baltimore, MD, ³Revivicor, Blacksburg, VA
*Purpose: Knock-out (KO) of porcine carbohydrates (CHO), including Gal 1,3αGal, inhibits antibody-mediated xenograft rejection. The β4Gal epitope was discovered using serum from xenograft-sensitized baboons, but…
2020 American Transplant Congress
In-Vivo Application of Ides (Imlifidase) in Nonhuman Primates Results in Xenoreactive Desensitization: A Novel Anti-Humoral Therapy
B. P. Lovasik¹, A. J. Matar¹, D. A. Faber¹, D. V. Mathews¹, C. Breeden¹, S. C. Kim¹, A. J. Tector², A. Robertson³, C. Kjellman³, A. B. Adams¹
¹Emory University School of Medicine, Atlanta, GA, ²University of Miami, Miami, FL, ³HANSA Biopharma, Lund, Sweden
*Purpose: Antibody-mediated rejection is a formidable barrier to xenograft survival. A novel anti-humoral IdeS therapy, which cleaves serum IgG, represents a promising therapy to reduce…
2020 American Transplant Congress
The Effect of B Cell Depletion Therapies on Preformed Xenoantibodies in Transplant Recipient
S. Yeh¹, N. A. Wilson², A. Djamali², R. Redfield³
¹Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, ²Medicine/Nephrology, University of Wisconsin-Madison, Madison, WI, ³Surgery, University of Wisconsin-Madison, Madison, WI
*Purpose: To assess the effect of B cell depletion therapies on preformed xenoantibodies in transplant recipient. Preformed xenoantibodies are a significant barrier to successful xenotransplantation.…
2020 American Transplant Congress
Sensitization via Pregnancy Generates Alloantibody and Cross-Reactive Xeno-Antibody in a Non-Human Primate Model
J. Yoon, M. Manook, Z. Fitch, R. Schmitz, A. Choi, A. M. Jackson, S. Knechtle, J. Kwun
Surgery, Duke University, Durham, NC
*Purpose: Women are disproportionately represented among highly sensitized waitlist candidates and within HLA-incompatible transplantation programs. Due to difficulties obtaining compatible donors, sensitized patients are seen…
2020 American Transplant Congress
Xenoantibody Depletion of Human Blood with Normothermic Machine Perfusion of Porcine Kidney and Lung Prior to Liver Perfusion
T. M. Coe¹, N. Serifis¹, S. Raigani¹, C. Carroll¹, C. G. Rickert¹, R. Matheson¹, D. Detelich², W. Qin³, Y. Kan³, J. Layer³, M. Youd³, W. Westlin³, L. Yang³, A. Azimzadeh¹, J. F. Markmann¹
¹Surgery, Massachusetts General Hospital, Boston, MA, ²Surgery, Tufts Medical Center, Boston, MA, ³eGenesis, Cambridge, MA
*Purpose: Genetic modifications of porcine donors for pig-to-human xenotransplantation have led to significant progress in survival of xenografts in preclinical models. Triple knock-out (TKO) of…
2020 American Transplant Congress
Old World Monkeys Are Not Ideal Transplantation Models for Testing CMAHKO (TKO or GTKO/CMAHKO) Pig Kidneys
T. Yamamoto¹, H. Iwase¹, D. Patel¹, A. Jagdale¹, J. Foote¹, D. Ayares², D. Anderson¹, D. E. Eckhoff¹, D. K. Cooper¹, H. Hara¹
¹University of Alabama at Birmingham, Birmingham, AL, ²Revivicor, Blacksburg, VA
*Purpose: Triple-knockout (TKO) pigs are likely to be an optimal source of organs for transplantation into human recipients, many of whom do not have natural…
2020 American Transplant Congress
Normothermic Machine Perfusion of Genetically Modified Pig Livers with Human Blood
D. Detelich¹, T. M. Coe², C. G. Rickert², C. Carroll², N. Serifis², R. Matheson², I. Rosales², W. Qin³, Y. Kan³, J. Layer³, M. Youd³, W. Westlin³, S. Kimura², A. Azimzadeh², L. Yang³, J. F. Markmann²
¹Tufts Medical Center, Boston, MA, ²Massachusetts General Hospital, Boston, MA, ³eGenesis, Cambridge, MA
*Purpose: Barriers to pig-to-human liver xenotransplantation remain, including profound thrombocytopenia, coagulopathy, and rejection. We utilized ex-vivo liver xenoperfusion with human blood to study the human…
2020 American Transplant Congress
Pig-To-Baboon Liver Xenotransplantation Utilizing Genetically Modified Swine
D. Detelich¹, C. G. Rickert¹, T. M. Coe¹, S. Kimura¹, I. Rosales¹, H. Deng¹, H. Yeh¹, W. Qin², Y. Kan², J. Layer², M. Youd², W. Westlin², L. Yang², J. F. Markmann¹
¹Massachusetts General Hospital, Boston, MA, ²eGenesis, Cambridge, MA
*Purpose: Pig-to-baboon liver xenotransplant (LXT) continues with significant hurdles, including thrombocytopenia, coagulation dysregulation and rejection with the longest reported survival of 29 days utilizing a…
2020 American Transplant Congress
Successful Long-Term TMA- and Rejection- Free Survival of a Kidney Xenograft with Triple Xenoantigen Knockout Plus Insertion of Multiple Human Transgenes
D. H. Ma¹, H. Sasaki¹, T. Hirose¹, I. Rosales¹, A. Dehnadi¹, R. Colvin¹, J. F. Markmann¹, W. Win², Y. Kan², J. Layer², M. Youd², W. Westlin², L. Yang², T. Kawai¹
¹Surgery, MGH, Boston, MA, ²eGenesis Inc., Cambridge, MA
*Purpose: Recent advances in genome engineering technology allow for the generation of porcine organ donors with multiplex gene edits. Here we evaluated survival of kidneys…