*Purpose: Adipose derived regenerative cells (ADRCs) are a heterogenous cell population derived from adipose tissue. These cells are composed of endothelial progenitor cells, pericytes, and adipose stromal cells. Previous studies showed that ADRCs have reparative effects through anti-inflammatory, and anti-fibrotic activity, and can potentially serve for the rehabilitation of solid organ transplants. We used hydrogen peroxide to induce injury in a rat proximal tubular cell line, NRK-52E cells, mimicking oxidative stress and damage of a transplant kidney. Our study investigates fibrosis-related mechanisms of ADRC renal repair.

*Methods: NRK-52E cells were assigned to four groups including non-injury, injury, ADRC-treated non-injury, and ADRC-treated injury groups. The cells were induced injury by incubation with a 500 µM hydrogen peroxide for 4 hours. ADRCs were added (10% ratio of total NRK-52E cells) and expression of target genes was assayed by real-time polymerase chain reaction (RT-qPCR) after 24 hours of ADRC treatment.

*Results: The ADRC-treated injured group had significantly decreased collagen type I (COL1) mRNA expression compared to non-injury group (p=0.0439) (figure 1) and had a significantly decreased neutrophil gelatinase-associated lipocalin (NGAL) mRNA expression compared to injury group (p=0.0015) (figure 2).

*Conclusions: This study showed that ADRCs have positive response by reducing kidney injury markers and by decreasing progenitor fibrotic factors of NRK-52E cells and implies ADRCs therapeutic remodelling of the extracellular matrix.

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