Biliary pH, Bicarbonate and Glucose Are Suitable Biomarkers of Bile Duct Viability during Normothermic Machine Perfusion of Human Donor Livers
1Department of Surgery, Section Hepatopancreatobiliary Surgery & Liver Transplantation, University Medical Center Groningen, University of Groningen, Groningen, Netherlands
2Surgical Research Laboratory, University Medical Center Groningen, University of Groningen, Groningen, Netherlands
3Department of Pathology, University Medical Center Groningen, University of Groningen, Groningen, Netherlands.
Meeting: 2018 American Transplant Congress
Abstract number: D41
Keywords: Bile duct, Liver transplantation, Machine preservation, Risk factors
Session Information
Session Name: Poster Session D: Ischemia Reperfusion Injury: Time to Change the Fate?
Session Type: Poster Session
Date: Tuesday, June 5, 2018
Session Time: 6:00pm-7:00pm
Presentation Time: 6:00pm-7:00pm
Location: Hall 4EF
Background: Normothermic machine perfusion (NMP) is rapidly making its way into the clinic and can be used for viability assessment of human donor livers prior to transplantation. Thus far, however, selection criteria have only been based on hepatocyte function and injury, and no criteria have been established to assess bile duct injury.Methods: Twenty-three human donor livers that were discarded for transplantation underwent a period of static cold storage and 6 hours of NMP upon arrival at our center. Biopsies of the extrahepatic bile duct were taken before and after NMP and subsequently semi-quantitatively scored using a modified scoring system. The median injury score between the two biopsies was used to divide livers into high and low biliary injury. Bile was collected during NMP for analyses.Results: Biliary pH and biliary bicarbonate concentration were significantly higher in livers with low biliary injury compared to livers with high biliary injury, and biliary glucose and biliary LDH concentration were significantly lower in livers with low biliary injury. A biliary pH above 7.4, biliary bicarbonate concentration above 25 mmol/L and biliary glucose concentration below 20 mmol/L at 2.5 hours of NMP, as well as a biliary LDH concentration below 5000 mmol/L already at 30 min of NMP, strongly predict low histological bile duct injury. Furthermore, livers with low biliary injury had relatively low glucose concentration in bile in relation to perfusate, whereas livers with high biliary injury showed approximately a 1:1 ratio between glucose concentration in bile and perfusate.Conclusions: This study provides hands-on parameters to assess bile duct injury during NMP, allowing researchers and surgeons to make better informed decisions in selecting donor livers that are suitable for transplantation.
CITATION INFORMATION: Matton A., de Vries Y., Burlage L., van Rijn R., Gouw A., de Meijer V., Lisman T., Porte R. Biliary pH, Bicarbonate and Glucose Are Suitable Biomarkers of Bile Duct Viability during Normothermic Machine Perfusion of Human Donor Livers Am J Transplant. 2017;17 (suppl 3).
To cite this abstract in AMA style:
Matton A, Vries Yde, Burlage L, Rijn Rvan, Gouw A, Meijer Vde, Lisman T, Porte R. Biliary pH, Bicarbonate and Glucose Are Suitable Biomarkers of Bile Duct Viability during Normothermic Machine Perfusion of Human Donor Livers [abstract]. https://atcmeetingabstracts.com/abstract/biliary-ph-bicarbonate-and-glucose-are-suitable-biomarkers-of-bile-duct-viability-during-normothermic-machine-perfusion-of-human-donor-livers/. Accessed May 16, 2025.« Back to 2018 American Transplant Congress