Previous study has shown that intravenous infusion of ethylene carbodiimide fixed donor splenocytes(ECDI-SPs) can induce tolerance of mice cardiac allografts with rapidly apoptosis of ECDI-SPs in the spleen of recipients. However, pro-inflammatory cytokine production during the peritransplantation period may negate the effect of ECDI-SPs. Therefore, we hypothesized that blocking pro-inflammatory cytokine secretion while increasing levels of anti-inflammatory cytokines would enhance the tolerance-induced efficacy of ECDI-SPs. The objective of this study was to investigate the efficacy of using ECDI-SPs combined with a short course of α1-antitrysin (AAT) for induction of tolerance. Using a mice cardiac transplant model, we demonstrated that ECDI-SPs plus AAT effectively induced indefinite mice cardiac allograft protection in a donor-specific fashion. This effect was accompanied by modulation of cytokines through decreasing levels of pro-inflammatory cytokines (including IFN-γ, TNF-α, IL-1β, IL-6, IL-17 and IL-23) and increasing levels of anti-inflammatory cytokines (including IL-10, IL-13 and TGF-β),and by inhibition of effector T cells (Teff) and expansion of regulatory T cells (Tregs).In conclusion, AAT appeared to modulate the expression of cytokines to create a support milieu to facilitate ECDI-SP inducing donor specific protection for mice cardiac allografts.

CITATION INFORMATION: Chen G., Lai X., Qiu L., Zhao Y., Yu S., Wang C., Zhang J., Ning F., Chen L. α-1 Antitrypsin Facilitate Apoptosis Donor Cells Inducing Donor Specific Protection to Mice Cardiac Allografts Am J Transplant. 2017;17 (suppl 3).

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