Noninvasive Detection of Infection and Rejection in Renal Transplant Patients

Y. Zhou,¹ Y. Cheng,¹ T. Chiang,² G. Yang,² X. Li,² F. Zeng,² X. Wang,¹ J. Ge,² X. Hu,² H. Shi,¹ T. Wang,² L. Liu.³

¹Laboratory of Transplant Genomics, Jiangsu University Institute of Life Science, ZhenJiang, JingSu, China
²Reasearch and Development Dpartment, SuZhou RRGene.Co.Ltd., SuZhou, JiangSu, China
³Department of Urological Organ Transplantation, The Second XiangYa Hospital of Center South University, ChangSha, Hunan, China.

Meeting: 2018 American Transplant Congress

Abstract number: A134

Keywords: Apoptosis, Kidney transplantation, Non-invasive diagnosis, Survival

Session Information

Session Name: Poster Session A: Kidney Acute Antibody Mediated Rejection

Session Type: Poster Session

Date: Saturday, June 2, 2018

Session Time: 5:30pm-7:30pm

Presentation Time: 5:30pm-7:30pm

Location: Hall 4EF

About 35% of patients would suffer actue rejection or infectionin in two years after reanl trandplantation.Cell-free DNA (cfDNA) is a new biomarker to evluate health of graft .The Graft-derived cfDNA (GdcfDNA) is direct correlation with acute rejection while pathogens' DNA detected in patients' plasm play role of indicator of infection.This study was to explore the feasibility of cfDNA in monitoring reanl transplant rejection and infection.

The GdcfDNA assay identifies low amounts of graft cfDNA with high linearity and accuracy .We selected 8, 000 SNPs and used target region capture sequencing technology to detect the genome copies (Gcs) derive from graft in the plasma (absolute quantitation) of 24 patients with renal transplantation. The results show 5 cases with clinical biopsy-proved of rejection as graft derived Gcs is 150.21±129.59(copies/ml). The graft derived Gcs of other 18 patients did not rejection is 43.69±23.61(copies/ml),there was a statistically significant difference between the two groups p<0.002 [Figure 1].- i.e. –

Metagenomic shotgun sequencing technology was applied to pathogens.The results showed that the method was effective in detecting DNA viruses, bacteria and fungi. In a biopsy-proved case with BK infection.Our result shows an identical match with clinical report,the number of supported reads is 40.Besides, in suspected infection case without clinical diagnosed, we detected 3 potential pathogenic organisms, including Streptococcaceae,CMV and Aspergillus.CMV and Streptococcaceae are validated doctor by applying CMV-igg test kit (dia.Pro Disgnostic Bioprobes,S.r.i.) and blood culture.

Our non-invasive method indicates effective in distinguish rejection and non-rejection,powerful identify pathogens in infection patients.

CITATION INFORMATION: Zhou Y., Cheng Y., Chiang T., Yang G., Li X., Zeng F., Wang X., Ge J., Hu X., Shi H., Wang T., Liu L. Noninvasive Detection of Infection and Rejection in Renal Transplant Patients Am J Transplant. 2017;17 (suppl 3).

To cite this abstract in AMA style:

Zhou Y, Cheng Y, Chiang T, Yang G, Li X, Zeng F, Wang X, Ge J, Hu X, Shi H, Wang T, Liu L. Noninvasive Detection of Infection and Rejection in Renal Transplant Patients [abstract]. https://atcmeetingabstracts.com/abstract/noninvasive-detection-of-infection-and-rejection-in-renal-transplant-patients/. Accessed November 22, 2024.

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