Intragraft MicroRNAs Foretell Fibrosis in Kidney Transplant Recipients
1Weill Cornell Medicine, New York
2Hôpital Necker, Paris, France
3Rockefeller University, New York.
Meeting: 2018 American Transplant Congress
Abstract number: A6
Keywords: Fibrosis, Gene expression, Kidney transplantation
Session Information
Session Name: Poster Session A: Biomarkers, Immune Monitoring and Outcomes
Session Type: Poster Session
Date: Saturday, June 2, 2018
Session Time: 5:30pm-7:30pm
Presentation Time: 5:30pm-7:30pm
Location: Hall 4EF
Background
Identifying biomarkers that anticipate tubulointerstitial fibrosis (IFTA) of the kidney allograft will be a transformative advance.
Aim/Hypothesis
Intragraft microRNA profile of 3-month surveillance biopsies reported as normal is associated with the presence of IFTA in 12-month surveillance biopsies.
Methods
Using RNA-sequencing, we studied the intragraft miRNA profile of 16 kidney allograft surveillance biopsies from 16 recipients at 3 months post–transplantation. None of the biopsies had IFTA (Banff ci/ct scores=0). Based on the presence of IFTA in the 12-month surveillance biopsy, 10 recipients were classified as 'Progressor' (ci/ct scores ≥2) and 6 as 'Non Progressor' (ci/ct scores=0). Two transplant pathologists reported the biopsies independently.
We compared the differential abundance of miRNA between Progressor and Non Progressor. We did qRT-PCR assay of the top differentially abundant miRNAs.
Results
Sixteen miRNAs were differentially abundant (fold change ≥2, P<0.05) between Progressor and Non Progressor. By qRT-PCR assay, the differential abundance of 3 miRNAs (miR-34a, miR-96 and miR-375) were statistically significant.
To assess the relevance of the 3 miRNAs in IFTA, we studied their intragraft abundance, by qRT-PCR assay, in 6 for-cause kidney allograft biopsies reported as IFTA and 6 surveillance biopsies reported as Normal. All 3 miRNAs were of higher abundance in IFTA (P<0.05) biopsies and had an AUC of >0.90 to distinguish IFTA from Normal.
Conclusion
Alterations in intragraft miRNA abundance in 3-month surveillance kidney allograft biopsies foretell the presence of IFTA in 12-month surveillance biopsies. The findings of our pilot study, if validated in a larger cohort, will be of considerable diagnostic and therapeutic significance.
CITATION INFORMATION: Yang H., Amrouche L., Akat K., Li C., Khan Z., Tinel C., Tuschl T., Anglicheau D., Muthukumar T. Intragraft MicroRNAs Foretell Fibrosis in Kidney Transplant Recipients Am J Transplant. 2017;17 (suppl 3).
To cite this abstract in AMA style:
Yang H, Amrouche L, Akat K, Li C, Khan Z, Tinel C, Tuschl T, Anglicheau D, Muthukumar T. Intragraft MicroRNAs Foretell Fibrosis in Kidney Transplant Recipients [abstract]. https://atcmeetingabstracts.com/abstract/intragraft-micrornas-foretell-fibrosis-in-kidney-transplant-recipients/. Accessed May 16, 2025.« Back to 2018 American Transplant Congress