Articles tagged "Xenotransplantation"

2016 American Transplant Congress
In Vivo and In Vitro Characterization of 1.5 mm Ba²⁺-Alginate Macro-Encapsulated Cynomolgus Monkey Islets.
M. Bochenek,¹ J. McGarrigle,¹ J. Mendoza-Elias,¹ E. Marchese,¹ M. Nourmohammadzadeh,¹ Y. Wang,¹ A. Khan,¹ M. Omami,¹ C.-C. Yeh,¹ I. Lacik,² B. Strand,³ J. Oberholzer.¹
¹Transplant Surgery, University of Iliinois at Chicago, Chicago, IL; ²Biomaterials Research, Polymer Institute of the Slovak Academy of Sciences, Bratislava, Slovakia (Slovak Republic); ³Biotechnology, Norwegian University of Science and Technology, Trondheim, Norway.
Introduction:Micro-encapsulation of islets for the treatment of Type I diabetes has shown limited efficacy in small-scale clinical trials. The lack of success may be related…
2016 American Transplant Congress
Establishment of SCID Pigs Cell Lines to Prevent Immunodeficiency Using RGEN System.
D.-H. Kim,¹ J.-S. Ahn,¹ S. Hwang,² J.-W. Lee.¹
¹Functional Genomics Reaserch Center, KRIBB, Daejeon, Korea; ²Animal Biotechnology Division, RDA, Wanju-gun, Jeollabuk-do, Korea.
Severe immune disorder is the biggest problem in xenotransplantation. To overcome the the problem, producing severe combined immune deficiency (SCID) pigs which lack T cell…
2016 American Transplant Congress
The Impact of Sequential Gene Modifications on Cardiac Xenograft Survival.
K. Horvath,¹ A. Singh,¹ J. Chan,¹ M. Thomas,² B. Lewis,² D. Ayares,³ P. Corcoran,¹ M. Mohiuddin.¹
¹CSRP, NHLBI/NIH, Bethesda, MD; ²DVR / ORS, NIH, Bethesda, MD; ³Revivicor, Inc, Blacksburg, VA.
Introduction: One of the major advances in xenotransplantation is the ability to modify donor pig genetics. Using rapidly evolving techniques of pig cloning and gene…
2016 American Transplant Congress
Inclusion of Human Tissue Factor Pathway Inhibitor or Thrombomodulin Transgenes Attenuates Critical Consumptive Thrombocytopenia in Cardiac Xenotransplantation.
J. Chan,¹ A. Singh,¹ P. Corcoran,¹ M. Thomas III,² B. Lewis,² D. Ayares,³ K. Horvath,¹ M. Mohiuddin.¹
¹Cardiothoracic Surgery Research Program/National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD; ²Division of Veterinary Resources, National Institutes of Health, Bethesda, MD; ³Revivicor Inc., Blacksburg, VA.
ObjectiveA major obstacle in the success of cardiac xenotransplantation has been the development of significant consumptive thrombocytopenia, frequently limiting recipient survival. Advances in genetic engineering…
2015 American Transplant Congress
Human Bone Marrow Mesenchymal Stem Cell-Derived Hepatocytes Express TIMP-4 and Follistatin
J. Li,¹ J. Xin,¹ W. Ding,² S. Hao,¹ L. Jiang,¹ Q. Zhou,¹ T. Wu,¹ D. Shi,¹ L. Li.¹
¹State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, HangZhou, China; ²Systems Biology Division, Zhejiang-California International Nanosystems Institute, Zhejiang University, HangZhou, China.
Human bone marrow mesenchymal stem cell (hBMSC) transplantation has been expected to be an alternative regenerative technique for liver diseases. However, the mechanism by which…
2015 American Transplant Congress
Effective Control of Anti-Xenogeneic IL-17 Response for Tolerance Induction in Xenogeneic Islet Cell Transplantation
H. Kang,¹ S. Wang,^1,2 X. Zhang,³ L. Zhang,¹ S. Miller,⁴ B. Hering,⁵ X. Luo.^1,3,4
¹Medicine, Northwestern University Feinberg school of Medicine, Chicago, IL; ²Tianjin Union Medical Center, Nankai University Affiliated Hospital, Tianjin, China; ³Comprehensive Transplant Center, Northwestern University Feinberg School of Medicine, Chicago, IL; ⁴Microbiology and Immunology, Northwestern University Feinberg School of Medicine, Chicago, IL; ⁵Schulze Diabetes Institute, University of Minnesota, Minneapolis, MN.
We have previously shown that peri-transplant infusion of donor cells treated with 1-ethyl-3-(3'-dimethylaminopropyl)-carbodiimide (ECDI) in combination with peri-transplant anti-CD20 and rapamycin induce permanent porcine islet…
2015 American Transplant Congress
Extended Life-Supporting Xenogeneic Lung Function With Multi-Transgeneic Donor Pigs and Targeted Drug Treatments
L. Burdorf,¹ E. Rybak,¹ T. Zhang,¹ D. Harris,¹ S. Dahi,¹ N. Kubicki,¹ D. Parsell,¹ E. Sievert,¹ D. Ayares,² A. Azimzadeh,¹ R. Pierson III.¹
¹University of Maryland, Baltimore; ²Revivicor, Blacksburg.
Purpose: Prolonged life-supporting (LS) xenolung function has proven difficult to accomplish. Here we report results from 21 xLTXs in a rigorous, LS pig-to-baboon model, asking…
2015 American Transplant Congress
Orthotopic Transplantation of a Tissue Engineered Skin Graft in Athymic Mice
N. Nayakawde, P. Patil, K. Methe, M. Olausson.
Surgery, Gothenburg University, Gothenburg, Vastra Gotaland, Sweden.
Background: Tissue engineered (TE) skin offers promising alternative treatment in wound healing of acute and chronic skin injuries. Limited autografts from donor skin and risks…
2015 American Transplant Congress
Identifying Barriers Specific to Xenoislets by Direct Comparison to Alloislets in a Dual Transplant Model
K. Samy,¹ B. Martin,² P. Thompson,² A. Farris,² M. Song,² V. Leopardi,¹ B. Collins,¹ A. Kirk.¹
¹Department of Surgery, Duke University Medical Center, Durham, NC; ²Emory Transplant Center, Emory University, Atlanta, GA.
Purpose:The xenotransplantation of neonatal porcine islets (NPIs) may alleviate the shortage of islets currently limiting islet allotransplantation. However, successful implementation requires understanding of the immune…
2015 American Transplant Congress
Detergent-Sonication Method for the Whole Laryngeal Decellularization
N. Nayakawde,¹ K. Methe,¹ M. Berg,² G. Premaratne,¹ H. Ejnell,² M. Olausson.¹
¹Surgery, Gothenburg University, Gothenburg, Vastra Gotaland, Sweden; ²Departments of 1Otolaryngology, Head and Neck Surgery, Gothenburg University, Gothenburg, Vastra Gotaland, Sweden.
Background: Each year, about 136,000 individuals worldwide are diagnosed with larynx carcinoma. Tissue engineered organs shows promising alternative to a conventional transplantation in both preclinical…