Articles tagged "Xenotransplantation"

2016 American Transplant Congress
Human Leukocyte Antigen-E Expression on Porcine GalTKO.hCD46 Cells Improves Ex-Vivo Xenograft Survival and Attenuates Injury.
C. Laird,¹ N. Kubicki,¹ L. Burdorf,¹ T. Zhang,¹ X. Chang,¹ G. Braileanu,¹ C. Phelps,² D. Ayares,² A. Azimzadeh,¹ R. Pierson.¹
¹University of Maryland School of Medicine, Baltimore, MD; ²Revivicor, Inc, Blacksburg, VA.
Purpose:Lung xenografts are subject to injury mechanisms associated with the sequestration of human cells, including natural killer (NK) cells. To control NK cell associated injury,…
2016 American Transplant Congress
Long-Term Islet Xenograft Survival Requires Effective Control of Early Xenogeneic IL-17 Response and Late Xenogeneic IFN-γ Response.
H. Kang,¹ S. Wang,² X. Zhang,³ A. Singh,⁵ L. Zhang,¹ W. Suarez-Pinzon,⁵ S. Miller,⁴ B. Hering,⁵ X. Luo.^1,3,4
¹Medicine, Northwestern University, Chicago, IL; ²Surgery, Tianjin Union Medical Center, Tianjin, China; ³Comprehensive Transplant Center, Northwestern University, Chicago, IL; ⁴Microbiology and Immunology, Northwestern University, Chicago, IL; ⁵Schulze Diabetes Institute, University of Minnesota, Minneapolis, MN.
Introduction: Tolerance induction for xenogeneic islet transplant remains a challenge. We have previously shown that peri-transplant infusion of donor cells treated with 1-ethyl-3-(3'-dimethylaminopropyl)-carbodiimide (ECDI) is…
2016 American Transplant Congress
Successful Human-to-NonHuman Primate Pancreatic Islet Intraportal Xenotransplantation Using a Human Allo-Based Immunosuppression Regimen and Its Potential Pre-Clinical Application.
J. McGarrigle,¹ M. Bochenek,¹ E. Marchese,¹ A. Khan,¹ D. Gutierrez,^1,2 A. Mikalauskaite,¹ B. McCracken,¹ M. Reedy,¹ M. Omami,¹ C.-C. Yeh,¹ M. Spaggiari,¹ M. Nourmohammadzadeh,^1,2 D. Isa,¹ S. Ghani,¹ L.-J. Wang,¹ J. Mendoza-Elias,^1,2 Y. Wang,¹ J. Oberholzer.^1,2
¹Surgery, University of Illinois at Chicago, Chicago; ²Bioengineering, University of Illinois at Chicago, Chicago.
Intraportal pancreatic islet cell transplantation is a successful treatment for type-1 diabetes (T1DM) in humans. Unfortunately, the lack of donor pancreatic organs is a major…
2016 American Transplant Congress
Multiplex Profiling of Plasma Proteins After Xenogenic Lung Exposure.
A. Azimzadeh,¹ N. Cheng,¹ X. Cheng,¹ E. Schwartz,¹ L. Burdorf,¹ S. Dahi,¹ N. Kubicki,¹ D. Parsell,¹ T. Zhang,¹ D. Cooper,² C. Phelps,³ D. Ayares,³ R. Pierson III.¹
¹Univ. Of Maryland, Baltimore, MD; ²University of Pittsburgh, Pittsburgh, PN; ³Revivicor, Blacksburg, VA.
Background: Porcine lungs exhibit injury by human blood and are only partially protected in GalTKO organs with additional human membrane cofactor protein (hCD46). Residual injury…
2015 American Transplant Congress
Detergent-Sonication Method for the Whole Laryngeal Decellularization
N. Nayakawde,¹ K. Methe,¹ M. Berg,² G. Premaratne,¹ H. Ejnell,² M. Olausson.¹
¹Surgery, Gothenburg University, Gothenburg, Vastra Gotaland, Sweden; ²Departments of 1Otolaryngology, Head and Neck Surgery, Gothenburg University, Gothenburg, Vastra Gotaland, Sweden.
Background: Each year, about 136,000 individuals worldwide are diagnosed with larynx carcinoma. Tissue engineered organs shows promising alternative to a conventional transplantation in both preclinical…
2015 American Transplant Congress
Characteristics of IgGs Produced in Neu5Gc and α1-3 Gal Double Knock-Out Pigs
A. Salama,^1,2 S. Conchon,¹ A. Perota,³ B. Martinet,¹ J.-P. Judor,¹ G. Evanno,¹ S. Le-Bas Bernardet,¹ L. Le Berre,¹ J. Hervouet,¹ D. Minault,¹ J.-P. Concordet,⁴ E. Dugast,¹ B. Vanhove,¹ J. Abadie,⁵ N. Gaide,⁵ I. Lagutina,³ R. Duchi,³ G. Lazzari,³ D. Sachs,⁶ O. Gauthier,⁷ S. Brouard,¹ E. Cozzi,⁸ G. Blancho,¹ H. Perreault,⁹ J.-M. Bach,¹⁰ C. Galli,^3,11 J.-P. Soulillou.¹
¹INSERM UMR 1064, ITUN, CHU de Nantes, Nantes University, Nantes, France; ²Société d'Accélération du Transfert de Technologies Ouest Valorisation, Rennes, France; ³Avantea, Laboratory of Reproductive Technologies, Cremona, Italy; ⁴INSERM U565, CNRS UMR7196, MNHN USM503, Paris, France; ⁵AMaROC, ONIRIS, Nantes, France; ⁶Massachusetts General Hospital, Transplantation Biology Research Center, Boston, MA; ⁷Centre de Recherche et d'Investigation Préclinique, ONIRIS, Nantes, France; ⁸Padua General Hospital and Consortium for Research in Organ Transplantation, Padua, Italy; ⁹Chemistry Department, University of Manitoba, Winnipeg, Canada; ¹⁰IECM, EA4644 University/ONIRIS USC1383 INRA, ONIRIS, Nantes, France; ¹¹Dept. of Veterinary Medical Sciences, University of Bologna, Bologna, Italy.
Xenotransplantation offers a large number of potential applications, including solid organs, pancreatic islets, acellular tissues such as heart valves and skin grafts, and new bioreagents…
2015 American Transplant Congress
Human Mesenchymal Stromal Cells Improve Survival and Function of Pancreatic Islets by Cell-to-Cell Contact
E. Montanari,¹ R. Mahou,^1,2 S. Passemard,² F. Noverraz,² P. Morel,¹ R. Meier,¹ D. Bosco,¹ J. Seebach,³ C. Wandrey,² S. Gerber,² C. Gonelle-Gispert,¹ L. Buhler.¹
¹Department of Surgery, Cell Isolation and Transplantation Center, Geneva University Hospitals and Medical Faculty, Geneva, Switzerland; ²Institut d'Ingénierie Biologique et Institut des Sciences et Ingénierie Chimiques, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland; ³Division of Immunology and Allergy, Geneva University Hospitals and Medical Faculty, Geneva, Switzerland.
Background and Aim:The aim of this study was to evaluate the survival and function of human pancreatic islets co-encapsulated with human Mesenchymal Stromal Cells (MSC)…
2015 American Transplant Congress
Infusion of Human Factor 7a Restores Circulating Platelets and Prevents TMA in Pig-to-Baboon Liver Xenotransplantation
J. Shah, N. Navarro-Alvarez, H. Yeh, N. Elias, J. Ligocka, J. Markmann, M. Hertl, D. Sachs, P. Vagefi.
Transplantation Biology Research Center, Massachusetts General Hospital/Harvard Medical School, Boston, MA.
Background We sought to determine the effects of exogenous administration of human coagulation factors in a pig-to-baboon model of liver xenotransplantation (LXT).Methods LXT was performed…
2015 American Transplant Congress
Cardiac Xenotransplantation: Reconstituted B Cells After Rituxan Treatment Maintain Their Repertoire But Have a Muted Response to Xenoantigens
A. Singh,¹ P. Corcoran,¹ B. Lewis,² M. Thomas,² D. Ayers,³ K. Horvath,¹ M. Mohiuddin.¹
¹CSRP/NIH/NHLBI, NIH, Bethesda, MD; ²DVR/ORS, NIH, Bethesda, MD; ³Revivicor Inc, Blacksburg, VA.
Background: Xentoranplantation (XTx) remains an option for end stage organ failure. Recently we have reported cardiac xenograft survival of over one year pig to baboon…
2015 American Transplant Congress
Immunological Evaluation of Acellular Extracellular Matrix Upon Transplantation
M. Ketaki,¹ P. Pradeep,¹ N. Nikhil,¹ G. Dellgren,³ H. Suchitra, M. Olausson.²
¹Laboratory for Transplantation Regenerative Medicine, Surgery, Gothenburg University, Gothenburg, Sweden; ²Department of Surgery, SU/Sahlgrenska University Hospital, SE 41345, Gothenburg, Sweden; ³Cardiothoracic Surgery, Transplant Institute, Sahlgrenska University Hospital, University of Gothenburg, Gothenburg, Sweden.
Background : To mitigate the scanty supply of donor organs, porcine tissues can be utilized since, it embrace the potential to accomplish the task of…