Articles tagged "Xenotransplantation"

2016 American Transplant Congress
CXCL12 as Innovative Immune Isolating Chemokine for Microencapsulated Allo and Xeno-Transplantation in NHPs.
M. Sremac,¹ A. Saraya,¹ O. Clemente,¹ M. Penson,¹ S. Chavan,³ N. Chronos,³ C. Schuetz,² J. Lei,² J. Markmann,² T. Brauns,¹ M. Poznansky.¹
¹Infectious Disease/VIC, Massachusetts General Hospital, Boston, MA; ²Division of Transplantation, Massachusetts General Hospital, Boston, MA; ³VICapsys, Inc, Athens, GA.
Introduction The purpose of this study is to determine whether a clinical-grade alginate-microencapsulant incorporating CXCL12, can protect and sustain the function of allo and/or adult…
2016 American Transplant Congress
Porcine Islets Engineered to Display SA-FasL Protein on Their Surface Induce Tolerance in Mice Following Transplantation into the Liver or Under the Kidney Capsule.
H. Zhao,¹ K. Woodward,¹ H. Shirwan,¹ M. Graham,² B. Hering,² E. Yolcu.¹
¹Institute for Cellular Therapeutics and Department of Microbiology and Immunology, University of Louisville, Louisville, KY; ²Schulze Diabetes Institute and Department of Surgery, University of Minnesota, Minneapolis, MN.
Introduction: We have recently demonstrated that allogeneic islets engineered to transiently display on their surface a novel form of FasL protein chimeric with streptavidin (SA-FasL)…
2016 American Transplant Congress
Eliminating Xenoantigen Expression on Swine Red Blood Cells.
Z.-Y. Wang,¹ G. Martens,¹ R. Blankenship,¹ R. Sidner,¹ P. Li,¹ J. Estrada,¹ M. Tector,² A. Tector.^1,3
¹Surgery, Indiana University School of Medicine, Indianapolis, IN; ²Surgery, Indiana University Health, Indianapolis, IN; ³Indiana University Health Transplant Institute, Indianapolis, IN.
Background:The rapidly improving tools of genetic engineering may make it possible to overcome humoral immune barrier that prevents xenotransplantation. We hypothesize that levels of human…
2016 American Transplant Congress
Impact of Mixed Xenogeneic Porcine Hematopoietic Chimerism on Human NK Cell Recognition in a Humanized Mouse Model.
H. Li,¹ P. Vishwasrao,^1,2 M. Holzl,¹ S. Chen,¹ G. Choi,^2,3 G. Zhao,¹ M. Sykes.¹
¹Columbia Center for Translational Immunology, Columbia University, New York, NY; ²Department of Hematology, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands; ³Department of Hematology, University Medical Center Groningen, Groningen, Netherlands.
Xenotransplantation is a potential solution for the shortage of organs for transplantation. However, potent xenograft rejection responses present a formidable barrier. Mixed xenogeneic hematopoietic chimerism…
2015 American Transplant Congress
Effective Control of Anti-Xenogeneic IL-17 Response for Tolerance Induction in Xenogeneic Islet Cell Transplantation
H. Kang,¹ S. Wang,^1,2 X. Zhang,³ L. Zhang,¹ S. Miller,⁴ B. Hering,⁵ X. Luo.^1,3,4
¹Medicine, Northwestern University Feinberg school of Medicine, Chicago, IL; ²Tianjin Union Medical Center, Nankai University Affiliated Hospital, Tianjin, China; ³Comprehensive Transplant Center, Northwestern University Feinberg School of Medicine, Chicago, IL; ⁴Microbiology and Immunology, Northwestern University Feinberg School of Medicine, Chicago, IL; ⁵Schulze Diabetes Institute, University of Minnesota, Minneapolis, MN.
We have previously shown that peri-transplant infusion of donor cells treated with 1-ethyl-3-(3'-dimethylaminopropyl)-carbodiimide (ECDI) in combination with peri-transplant anti-CD20 and rapamycin induce permanent porcine islet…
2015 American Transplant Congress
Extended Life-Supporting Xenogeneic Lung Function With Multi-Transgeneic Donor Pigs and Targeted Drug Treatments
L. Burdorf,¹ E. Rybak,¹ T. Zhang,¹ D. Harris,¹ S. Dahi,¹ N. Kubicki,¹ D. Parsell,¹ E. Sievert,¹ D. Ayares,² A. Azimzadeh,¹ R. Pierson III.¹
¹University of Maryland, Baltimore; ²Revivicor, Blacksburg.
Purpose: Prolonged life-supporting (LS) xenolung function has proven difficult to accomplish. Here we report results from 21 xLTXs in a rigorous, LS pig-to-baboon model, asking…
2015 American Transplant Congress
Orthotopic Transplantation of a Tissue Engineered Skin Graft in Athymic Mice
N. Nayakawde, P. Patil, K. Methe, M. Olausson.
Surgery, Gothenburg University, Gothenburg, Vastra Gotaland, Sweden.
Background: Tissue engineered (TE) skin offers promising alternative treatment in wound healing of acute and chronic skin injuries. Limited autografts from donor skin and risks…
2015 American Transplant Congress
Identifying Barriers Specific to Xenoislets by Direct Comparison to Alloislets in a Dual Transplant Model
K. Samy,¹ B. Martin,² P. Thompson,² A. Farris,² M. Song,² V. Leopardi,¹ B. Collins,¹ A. Kirk.¹
¹Department of Surgery, Duke University Medical Center, Durham, NC; ²Emory Transplant Center, Emory University, Atlanta, GA.
Purpose:The xenotransplantation of neonatal porcine islets (NPIs) may alleviate the shortage of islets currently limiting islet allotransplantation. However, successful implementation requires understanding of the immune…
2015 American Transplant Congress
Detergent-Sonication Method for the Whole Laryngeal Decellularization
N. Nayakawde,¹ K. Methe,¹ M. Berg,² G. Premaratne,¹ H. Ejnell,² M. Olausson.¹
¹Surgery, Gothenburg University, Gothenburg, Vastra Gotaland, Sweden; ²Departments of 1Otolaryngology, Head and Neck Surgery, Gothenburg University, Gothenburg, Vastra Gotaland, Sweden.
Background: Each year, about 136,000 individuals worldwide are diagnosed with larynx carcinoma. Tissue engineered organs shows promising alternative to a conventional transplantation in both preclinical…
2015 American Transplant Congress
Characteristics of IgGs Produced in Neu5Gc and α1-3 Gal Double Knock-Out Pigs
A. Salama,^1,2 S. Conchon,¹ A. Perota,³ B. Martinet,¹ J.-P. Judor,¹ G. Evanno,¹ S. Le-Bas Bernardet,¹ L. Le Berre,¹ J. Hervouet,¹ D. Minault,¹ J.-P. Concordet,⁴ E. Dugast,¹ B. Vanhove,¹ J. Abadie,⁵ N. Gaide,⁵ I. Lagutina,³ R. Duchi,³ G. Lazzari,³ D. Sachs,⁶ O. Gauthier,⁷ S. Brouard,¹ E. Cozzi,⁸ G. Blancho,¹ H. Perreault,⁹ J.-M. Bach,¹⁰ C. Galli,^3,11 J.-P. Soulillou.¹
¹INSERM UMR 1064, ITUN, CHU de Nantes, Nantes University, Nantes, France; ²Société d'Accélération du Transfert de Technologies Ouest Valorisation, Rennes, France; ³Avantea, Laboratory of Reproductive Technologies, Cremona, Italy; ⁴INSERM U565, CNRS UMR7196, MNHN USM503, Paris, France; ⁵AMaROC, ONIRIS, Nantes, France; ⁶Massachusetts General Hospital, Transplantation Biology Research Center, Boston, MA; ⁷Centre de Recherche et d'Investigation Préclinique, ONIRIS, Nantes, France; ⁸Padua General Hospital and Consortium for Research in Organ Transplantation, Padua, Italy; ⁹Chemistry Department, University of Manitoba, Winnipeg, Canada; ¹⁰IECM, EA4644 University/ONIRIS USC1383 INRA, ONIRIS, Nantes, France; ¹¹Dept. of Veterinary Medical Sciences, University of Bologna, Bologna, Italy.
Xenotransplantation offers a large number of potential applications, including solid organs, pancreatic islets, acellular tissues such as heart valves and skin grafts, and new bioreagents…