Articles tagged "Xenotransplantation"

2016 American Transplant Congress
Comparison of Early Engraftment Immunobiology of Xenoislets and Alloislets in a Dual Transplant Model.
K. Samy,¹ B. Martin,² M. Song,¹ B. Collins,¹ E. Gall,¹ J. Cano,² A. Farris,² F. Leopardi,¹ A. Kirk.^1,2
¹Department of Surgery, Duke University Medical Center, Durham, NC; ²Emory University, Atlanta, GA.
Purpose: Successful implementation of neonatal porcine islet (NPI) xenotransplantation requires an understanding of the xenospecific immune elements influencing engraftment. To elucidate these early xenospecific processes,…
2016 American Transplant Congress
CXCL12 as Innovative Immune Isolating Chemokine for Microencapsulated Allo and Xeno-Transplantation in NHPs.
M. Sremac,¹ A. Saraya,¹ O. Clemente,¹ M. Penson,¹ S. Chavan,³ N. Chronos,³ C. Schuetz,² J. Lei,² J. Markmann,² T. Brauns,¹ M. Poznansky.¹
¹Infectious Disease/VIC, Massachusetts General Hospital, Boston, MA; ²Division of Transplantation, Massachusetts General Hospital, Boston, MA; ³VICapsys, Inc, Athens, GA.
Introduction The purpose of this study is to determine whether a clinical-grade alginate-microencapsulant incorporating CXCL12, can protect and sustain the function of allo and/or adult…
2016 American Transplant Congress
Porcine Islets Engineered to Display SA-FasL Protein on Their Surface Induce Tolerance in Mice Following Transplantation into the Liver or Under the Kidney Capsule.
H. Zhao,¹ K. Woodward,¹ H. Shirwan,¹ M. Graham,² B. Hering,² E. Yolcu.¹
¹Institute for Cellular Therapeutics and Department of Microbiology and Immunology, University of Louisville, Louisville, KY; ²Schulze Diabetes Institute and Department of Surgery, University of Minnesota, Minneapolis, MN.
Introduction: We have recently demonstrated that allogeneic islets engineered to transiently display on their surface a novel form of FasL protein chimeric with streptavidin (SA-FasL)…
2016 American Transplant Congress
Eliminating Xenoantigen Expression on Swine Red Blood Cells.
Z.-Y. Wang,¹ G. Martens,¹ R. Blankenship,¹ R. Sidner,¹ P. Li,¹ J. Estrada,¹ M. Tector,² A. Tector.^1,3
¹Surgery, Indiana University School of Medicine, Indianapolis, IN; ²Surgery, Indiana University Health, Indianapolis, IN; ³Indiana University Health Transplant Institute, Indianapolis, IN.
Background:The rapidly improving tools of genetic engineering may make it possible to overcome humoral immune barrier that prevents xenotransplantation. We hypothesize that levels of human…
2016 American Transplant Congress
Impact of Mixed Xenogeneic Porcine Hematopoietic Chimerism on Human NK Cell Recognition in a Humanized Mouse Model.
H. Li,¹ P. Vishwasrao,^1,2 M. Holzl,¹ S. Chen,¹ G. Choi,^2,3 G. Zhao,¹ M. Sykes.¹
¹Columbia Center for Translational Immunology, Columbia University, New York, NY; ²Department of Hematology, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands; ³Department of Hematology, University Medical Center Groningen, Groningen, Netherlands.
Xenotransplantation is a potential solution for the shortage of organs for transplantation. However, potent xenograft rejection responses present a formidable barrier. Mixed xenogeneic hematopoietic chimerism…
2016 American Transplant Congress
Long Term Heterotopic Cardiac Xenograft Survival from Donor Pigs with Six Gene Modifications.
M. Mohiuddin,¹ A. Singh,¹ J. Chan,¹ P. Corcoran,¹ M. Thomas III,² B. Lewis,² D. Ayares,³ K. Horvath.¹
¹CSRP, NHLBI/NIH, Bethesda, MD; ²DVR / RS, NIH, Bethesda, MD; ³Revivicor, Inc, Blacksburgh, VA.
Introduction: We have recently reported pig xenograft survival of more than 2.5 years in a heterotopic cardiac xenograft model. Some of the animals exhibited local…
2016 American Transplant Congress
Human Leukocyte Antigen-E Expression on Porcine GalTKO.hCD46 Cells Improves Ex-Vivo Xenograft Survival and Attenuates Injury.
C. Laird,¹ N. Kubicki,¹ L. Burdorf,¹ T. Zhang,¹ X. Chang,¹ G. Braileanu,¹ C. Phelps,² D. Ayares,² A. Azimzadeh,¹ R. Pierson.¹
¹University of Maryland School of Medicine, Baltimore, MD; ²Revivicor, Inc, Blacksburg, VA.
Purpose:Lung xenografts are subject to injury mechanisms associated with the sequestration of human cells, including natural killer (NK) cells. To control NK cell associated injury,…
2016 American Transplant Congress
Long-Term Islet Xenograft Survival Requires Effective Control of Early Xenogeneic IL-17 Response and Late Xenogeneic IFN-γ Response.
H. Kang,¹ S. Wang,² X. Zhang,³ A. Singh,⁵ L. Zhang,¹ W. Suarez-Pinzon,⁵ S. Miller,⁴ B. Hering,⁵ X. Luo.^1,3,4
¹Medicine, Northwestern University, Chicago, IL; ²Surgery, Tianjin Union Medical Center, Tianjin, China; ³Comprehensive Transplant Center, Northwestern University, Chicago, IL; ⁴Microbiology and Immunology, Northwestern University, Chicago, IL; ⁵Schulze Diabetes Institute, University of Minnesota, Minneapolis, MN.
Introduction: Tolerance induction for xenogeneic islet transplant remains a challenge. We have previously shown that peri-transplant infusion of donor cells treated with 1-ethyl-3-(3'-dimethylaminopropyl)-carbodiimide (ECDI) is…
2016 American Transplant Congress
Successful Human-to-NonHuman Primate Pancreatic Islet Intraportal Xenotransplantation Using a Human Allo-Based Immunosuppression Regimen and Its Potential Pre-Clinical Application.
J. McGarrigle,¹ M. Bochenek,¹ E. Marchese,¹ A. Khan,¹ D. Gutierrez,^1,2 A. Mikalauskaite,¹ B. McCracken,¹ M. Reedy,¹ M. Omami,¹ C.-C. Yeh,¹ M. Spaggiari,¹ M. Nourmohammadzadeh,^1,2 D. Isa,¹ S. Ghani,¹ L.-J. Wang,¹ J. Mendoza-Elias,^1,2 Y. Wang,¹ J. Oberholzer.^1,2
¹Surgery, University of Illinois at Chicago, Chicago; ²Bioengineering, University of Illinois at Chicago, Chicago.
Intraportal pancreatic islet cell transplantation is a successful treatment for type-1 diabetes (T1DM) in humans. Unfortunately, the lack of donor pancreatic organs is a major…
2016 American Transplant Congress
Multiplex Profiling of Plasma Proteins After Xenogenic Lung Exposure.
A. Azimzadeh,¹ N. Cheng,¹ X. Cheng,¹ E. Schwartz,¹ L. Burdorf,¹ S. Dahi,¹ N. Kubicki,¹ D. Parsell,¹ T. Zhang,¹ D. Cooper,² C. Phelps,³ D. Ayares,³ R. Pierson III.¹
¹Univ. Of Maryland, Baltimore, MD; ²University of Pittsburgh, Pittsburgh, PN; ³Revivicor, Blacksburg, VA.
Background: Porcine lungs exhibit injury by human blood and are only partially protected in GalTKO organs with additional human membrane cofactor protein (hCD46). Residual injury…