Articles tagged "Xenotransplantation"

2016 American Transplant Congress
Porcine Islets Engineered with SA-FasL Protein Induces Robust Tolerance in Mice through CD4⁺CD25⁺FoxP3⁺ Treg Cells.
K. Woodward,¹ H. Zhao,¹ H. Shirwan,¹ F. Wang,¹ M. Graham,² B. Hering,² E. Yolcu.¹
¹Institute for Cellular Therapeutics and Department of Microbiology and Immunology, University of Louisville, Louisville, KY; ²Schulze Diabetes Institute and Department of Surgery, University of Minnesota, Minneapolis, MN.
Introduction: We have shown that porcine islets engineered to transiently display SA-FasL protein on their surface establish tolerance in mice following transplantation under the kidney…
2016 American Transplant Congress
An Improved Flow Cytometry Based Platelet Counting in Xeno Perfusion Models.
S. Sendil,¹ G. Braileanu,¹ W. Sun,¹ W. Hassanein,¹ D. Parsell,¹ H. Crum,² L. Burdorf,¹ R. Pierson,¹ A. Azimzadeh.¹
¹Department of Surgery, University of Maryland School of Medicine, Baltimore, MD; ²VA Medical Center, Baltimore, MD.
Aim: Human or baboon platelet sequestration and activation occurs during pig organ xenograft perfusion; its mechanism remains poorly understood. In the context of a regimen…
2016 American Transplant Congress
aPC Pretreatment of hEPCR-Expressing PAECs Attenuates Endothelial Cell Damage and Thrombosis.
C. Laird,¹ B. French,¹ D. Harris,¹ X. Chang,¹ D. Ayares,² R. Pierson,¹ A. Azimzadeh.¹
¹Cardiac Surgery, University of Maryland School of Medicine, Baltimore, MD; ²Revivicor, Inc, Blacksburg, VA.
IntroductionActivated Protein C (aPC), acting primarily through the human endothelial protein C receptor (hEPCR), mediates anti-thrombotic and anti-inflammatory cytoprotective effects in humans. We hypothesized that…
2016 American Transplant Congress
In Vivo and In Vitro Characterization of 1.5 mm Ba²⁺-Alginate Macro-Encapsulated Cynomolgus Monkey Islets.
M. Bochenek,¹ J. McGarrigle,¹ J. Mendoza-Elias,¹ E. Marchese,¹ M. Nourmohammadzadeh,¹ Y. Wang,¹ A. Khan,¹ M. Omami,¹ C.-C. Yeh,¹ I. Lacik,² B. Strand,³ J. Oberholzer.¹
¹Transplant Surgery, University of Iliinois at Chicago, Chicago, IL; ²Biomaterials Research, Polymer Institute of the Slovak Academy of Sciences, Bratislava, Slovakia (Slovak Republic); ³Biotechnology, Norwegian University of Science and Technology, Trondheim, Norway.
Introduction:Micro-encapsulation of islets for the treatment of Type I diabetes has shown limited efficacy in small-scale clinical trials. The lack of success may be related…
2016 American Transplant Congress
Establishment of SCID Pigs Cell Lines to Prevent Immunodeficiency Using RGEN System.
D.-H. Kim,¹ J.-S. Ahn,¹ S. Hwang,² J.-W. Lee.¹
¹Functional Genomics Reaserch Center, KRIBB, Daejeon, Korea; ²Animal Biotechnology Division, RDA, Wanju-gun, Jeollabuk-do, Korea.
Severe immune disorder is the biggest problem in xenotransplantation. To overcome the the problem, producing severe combined immune deficiency (SCID) pigs which lack T cell…
2016 American Transplant Congress
The Impact of Sequential Gene Modifications on Cardiac Xenograft Survival.
K. Horvath,¹ A. Singh,¹ J. Chan,¹ M. Thomas,² B. Lewis,² D. Ayares,³ P. Corcoran,¹ M. Mohiuddin.¹
¹CSRP, NHLBI/NIH, Bethesda, MD; ²DVR / ORS, NIH, Bethesda, MD; ³Revivicor, Inc, Blacksburg, VA.
Introduction: One of the major advances in xenotransplantation is the ability to modify donor pig genetics. Using rapidly evolving techniques of pig cloning and gene…
2016 American Transplant Congress
Inclusion of Human Tissue Factor Pathway Inhibitor or Thrombomodulin Transgenes Attenuates Critical Consumptive Thrombocytopenia in Cardiac Xenotransplantation.
J. Chan,¹ A. Singh,¹ P. Corcoran,¹ M. Thomas III,² B. Lewis,² D. Ayares,³ K. Horvath,¹ M. Mohiuddin.¹
¹Cardiothoracic Surgery Research Program/National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD; ²Division of Veterinary Resources, National Institutes of Health, Bethesda, MD; ³Revivicor Inc., Blacksburg, VA.
ObjectiveA major obstacle in the success of cardiac xenotransplantation has been the development of significant consumptive thrombocytopenia, frequently limiting recipient survival. Advances in genetic engineering…
2016 American Transplant Congress
Costimulation Blockade by Anti CD40 Antibody Prevents Humoral and Cellular Rejection of Heterotopic Pig Cardiac Xenograft.
A. Singh,¹ J. Chan,¹ P. Corcoran,¹ B. Lewis,² M. Thomas,² D. Ayares,³ K. Horvath,¹ M. Mohiuddin.¹
¹CSRP/NHLBI, NIH, Bethesda, MD; ²DVR/ORS, NIH, Bethesda, MD; ³Revivicor Inc., Blacksburg, VA.
BackgroundXenotransplantation (XTx) involves the participation of innate immune system with natural antibody producing B cells, NK cells, macrophages and complement as well as adaptive immune…
2016 American Transplant Congress
Late Renal Xenograft Failure Is Antibody-Mediated: Description of the Longest-Reported Survival in Pig-to-Primate Renal Xenotransplantation.
L. Higginbotham,¹ S. Kim,¹ D. Mathews,¹ A. Stephenson,¹ C. Breeden,¹ C. Larsen,¹ M. Ford,¹ K. Newell,¹ A. Tector,² A. Adams.¹
¹Emory Transplant Center, Emory University Hospital, Atlanta, GA; ²Indiana University School of Medicine, Indianapolis, IN.
Introduction. Xenotransplantation has the potential to alleviate the organ supply shortage, yet pig-to-primate renal xenotransplant has met limited success due to natural preformed anti-pig antibody.…
2016 American Transplant Congress
Comparison of Early Engraftment Immunobiology of Xenoislets and Alloislets in a Dual Transplant Model.
K. Samy,¹ B. Martin,² M. Song,¹ B. Collins,¹ E. Gall,¹ J. Cano,² A. Farris,² F. Leopardi,¹ A. Kirk.^1,2
¹Department of Surgery, Duke University Medical Center, Durham, NC; ²Emory University, Atlanta, GA.
Purpose: Successful implementation of neonatal porcine islet (NPI) xenotransplantation requires an understanding of the xenospecific immune elements influencing engraftment. To elucidate these early xenospecific processes,…