Articles tagged "Xenotransplantation"

2016 American Transplant Congress
The Impact of Sequential Gene Modifications on Cardiac Xenograft Survival.
K. Horvath,¹ A. Singh,¹ J. Chan,¹ M. Thomas,² B. Lewis,² D. Ayares,³ P. Corcoran,¹ M. Mohiuddin.¹
¹CSRP, NHLBI/NIH, Bethesda, MD; ²DVR / ORS, NIH, Bethesda, MD; ³Revivicor, Inc, Blacksburg, VA.
Introduction: One of the major advances in xenotransplantation is the ability to modify donor pig genetics. Using rapidly evolving techniques of pig cloning and gene…
2016 American Transplant Congress
Inclusion of Human Tissue Factor Pathway Inhibitor or Thrombomodulin Transgenes Attenuates Critical Consumptive Thrombocytopenia in Cardiac Xenotransplantation.
J. Chan,¹ A. Singh,¹ P. Corcoran,¹ M. Thomas III,² B. Lewis,² D. Ayares,³ K. Horvath,¹ M. Mohiuddin.¹
¹Cardiothoracic Surgery Research Program/National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD; ²Division of Veterinary Resources, National Institutes of Health, Bethesda, MD; ³Revivicor Inc., Blacksburg, VA.
ObjectiveA major obstacle in the success of cardiac xenotransplantation has been the development of significant consumptive thrombocytopenia, frequently limiting recipient survival. Advances in genetic engineering…
2016 American Transplant Congress
Costimulation Blockade by Anti CD40 Antibody Prevents Humoral and Cellular Rejection of Heterotopic Pig Cardiac Xenograft.
A. Singh,¹ J. Chan,¹ P. Corcoran,¹ B. Lewis,² M. Thomas,² D. Ayares,³ K. Horvath,¹ M. Mohiuddin.¹
¹CSRP/NHLBI, NIH, Bethesda, MD; ²DVR/ORS, NIH, Bethesda, MD; ³Revivicor Inc., Blacksburg, VA.
BackgroundXenotransplantation (XTx) involves the participation of innate immune system with natural antibody producing B cells, NK cells, macrophages and complement as well as adaptive immune…
2016 American Transplant Congress
Late Renal Xenograft Failure Is Antibody-Mediated: Description of the Longest-Reported Survival in Pig-to-Primate Renal Xenotransplantation.
L. Higginbotham,¹ S. Kim,¹ D. Mathews,¹ A. Stephenson,¹ C. Breeden,¹ C. Larsen,¹ M. Ford,¹ K. Newell,¹ A. Tector,² A. Adams.¹
¹Emory Transplant Center, Emory University Hospital, Atlanta, GA; ²Indiana University School of Medicine, Indianapolis, IN.
Introduction. Xenotransplantation has the potential to alleviate the organ supply shortage, yet pig-to-primate renal xenotransplant has met limited success due to natural preformed anti-pig antibody.…
2016 American Transplant Congress
Comparison of Early Engraftment Immunobiology of Xenoislets and Alloislets in a Dual Transplant Model.
K. Samy,¹ B. Martin,² M. Song,¹ B. Collins,¹ E. Gall,¹ J. Cano,² A. Farris,² F. Leopardi,¹ A. Kirk.^1,2
¹Department of Surgery, Duke University Medical Center, Durham, NC; ²Emory University, Atlanta, GA.
Purpose: Successful implementation of neonatal porcine islet (NPI) xenotransplantation requires an understanding of the xenospecific immune elements influencing engraftment. To elucidate these early xenospecific processes,…
2016 American Transplant Congress
CXCL12 as Innovative Immune Isolating Chemokine for Microencapsulated Allo and Xeno-Transplantation in NHPs.
M. Sremac,¹ A. Saraya,¹ O. Clemente,¹ M. Penson,¹ S. Chavan,³ N. Chronos,³ C. Schuetz,² J. Lei,² J. Markmann,² T. Brauns,¹ M. Poznansky.¹
¹Infectious Disease/VIC, Massachusetts General Hospital, Boston, MA; ²Division of Transplantation, Massachusetts General Hospital, Boston, MA; ³VICapsys, Inc, Athens, GA.
Introduction The purpose of this study is to determine whether a clinical-grade alginate-microencapsulant incorporating CXCL12, can protect and sustain the function of allo and/or adult…
2016 American Transplant Congress
Porcine Islets Engineered to Display SA-FasL Protein on Their Surface Induce Tolerance in Mice Following Transplantation into the Liver or Under the Kidney Capsule.
H. Zhao,¹ K. Woodward,¹ H. Shirwan,¹ M. Graham,² B. Hering,² E. Yolcu.¹
¹Institute for Cellular Therapeutics and Department of Microbiology and Immunology, University of Louisville, Louisville, KY; ²Schulze Diabetes Institute and Department of Surgery, University of Minnesota, Minneapolis, MN.
Introduction: We have recently demonstrated that allogeneic islets engineered to transiently display on their surface a novel form of FasL protein chimeric with streptavidin (SA-FasL)…
2016 American Transplant Congress
Eliminating Xenoantigen Expression on Swine Red Blood Cells.
Z.-Y. Wang,¹ G. Martens,¹ R. Blankenship,¹ R. Sidner,¹ P. Li,¹ J. Estrada,¹ M. Tector,² A. Tector.^1,3
¹Surgery, Indiana University School of Medicine, Indianapolis, IN; ²Surgery, Indiana University Health, Indianapolis, IN; ³Indiana University Health Transplant Institute, Indianapolis, IN.
Background:The rapidly improving tools of genetic engineering may make it possible to overcome humoral immune barrier that prevents xenotransplantation. We hypothesize that levels of human…
2016 American Transplant Congress
Impact of Mixed Xenogeneic Porcine Hematopoietic Chimerism on Human NK Cell Recognition in a Humanized Mouse Model.
H. Li,¹ P. Vishwasrao,^1,2 M. Holzl,¹ S. Chen,¹ G. Choi,^2,3 G. Zhao,¹ M. Sykes.¹
¹Columbia Center for Translational Immunology, Columbia University, New York, NY; ²Department of Hematology, Academic Medical Center, University of Amsterdam, Amsterdam, Netherlands; ³Department of Hematology, University Medical Center Groningen, Groningen, Netherlands.
Xenotransplantation is a potential solution for the shortage of organs for transplantation. However, potent xenograft rejection responses present a formidable barrier. Mixed xenogeneic hematopoietic chimerism…
2016 American Transplant Congress
Long Term Heterotopic Cardiac Xenograft Survival from Donor Pigs with Six Gene Modifications.
M. Mohiuddin,¹ A. Singh,¹ J. Chan,¹ P. Corcoran,¹ M. Thomas III,² B. Lewis,² D. Ayares,³ K. Horvath.¹
¹CSRP, NHLBI/NIH, Bethesda, MD; ²DVR / RS, NIH, Bethesda, MD; ³Revivicor, Inc, Blacksburgh, VA.
Introduction: We have recently reported pig xenograft survival of more than 2.5 years in a heterotopic cardiac xenograft model. Some of the animals exhibited local…