Articles tagged "Xenoreactive antibodies"

2019 American Transplant Congress
Islet Xenotransplantation: A Quest for Clinically Available Immunosuppression Regimens
Z. W. Fitch¹, Q. Gao¹, R. P. Davis¹, M. S. Mulvihill¹, M. Song¹, F. Leopardi¹, M. Ribeiro¹, T. How¹, K. Reimann², G. R. Devi¹, B. H. Collins¹, A. D. Kirk¹
¹Surgery, Duke University, Durham, NC, ²University of Massachusetts, Worcester, MA
*Purpose: Porcine islet xenotransplantation has progressed significantly in non-human primate (NHP) studies. However, all successful NHP studies have relied upon immunosuppressive regimens that utilize drugs…
2019 American Transplant Congress
Absence of Antibodies in Human Neonates and Infants to Gtko/cmahko/β4galnt2ko (Triple Gene Knockout) Pig Cells: Relevance to Cardiac Xenotransplantation for Complex Congenital Heart Disease
H. Hara¹, Q. Li¹, C. A. Banks¹, T. Yamamoto¹, D. Ayares², D. C. Cleveland¹, D. K. Cooper¹
¹Surgery, University of Alabama at Birmingham, Birmingham, AL, ²Revivicor Inc., Blacksburg, VA
*Purpose: Heart transplantation (Tx) in neonates is associated with the best results of any organ Tx, but the lack of deceased human donor hearts in…
2019 American Transplant Congress
The Effect of Intravenous Immunoglobulin on Human Complement-Dependent Cytotoxicity of Pig Cells: Benefit? Or Harm?
T. Yamamoto¹, Q. Li¹, A. Banks¹, H. Iwase¹, D. Ayares², D. K. Cooper¹, H. Hara¹
¹Xenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, Birmingham, AL, ²Revivicor, Blacksburg, VA
*Purpose: Xenoreactive antibodies and complement play an important role in pig xenograft rejection. Intravenous immunoglobulin (IVIG) is widely used in desensitization protocols and for the…
2019 American Transplant Congress
α -1,3-galactosyltransferase Knockout Porcine Tissue And Organ May Reduce The Risks Of Antibody Mediated Rejection Via Less Rhesus Igm Cross-reactivity.
J. Yoon, B. Ezekian, M. Manook, J. Kwun, S. Knechtle
Duke Transplant Center, Durham, NC
*Purpose: Previously, we observed that sera from rhesus macaques contained preformed antibodies against wild type (WT) swine splenocytes. In the context of xenotransplantation, preformed xenoantibodies…
2019 American Transplant Congress
Inhibition of Sialidase Activity and Galectin Binding Reduces Xenogeneic Neutrophil-Endothelial Adhesion
B. M. Cerel¹, B. M. French², D. G. Harris², C. Laird², L. Burdorf¹, X. Cheng², C. Feng³, A. S. Cross⁴, S. E. Goldblum⁴, G. R. Vasta³, R. N. Pierson III¹, A. M. Azimzadeh¹
¹Deparment of Surgery, Massachusetts General Hospital, Boston, MA, ²Department of Surgery, University of Maryland School of Medicine, Baltimore, MD, ³Department of Microbiology and Immunology, and Institute of Marine and Environmental Technology, University of Maryland School of Medicine, Baltimore, MD, ⁴Department of Medicine Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD
*Purpose: Immediate neutrophil sequestration occurs despite genetic modifications (Gal-a-1-3 galactosyl transferase knock out; human CD46 complement regulatory gene addition) to pig donor organs. Sialic acids…
2018 American Transplant Congress
Selection and Stratification of Anti-HLA-A and HLA-B Sensitized Patients in Pig-to-Human Xenotransplantation
G. Martens,¹ J. Ladowski,¹ J. Butler,² L. Reyes,¹ M. Tector,¹ A. Tector.¹
¹University of Alabama at Birmingham, Birmingham, AL; ²Indiana University School of Medicine, Indianapolis, IN.
Introduction: Patients with anti-HLA-A and –B antibodies are difficult to match and represent a possible population for inclusion in pig-to-human xenotransplantation clinical trials. Donor specific…
2018 American Transplant Congress
Rhesus Monkey Have natural Antibody toward Swine That is Not Influenced by Allosensitization nor by Carfilzomib/tocilizumab
J. Yoon, B. Ezekian, M. Manook, J. Kwun, S. Knechtle.
Surgery, Duke University, Durham, NC.
[Background] Pre-formed antibody is a substantial immunological barrier in organ transplantion. Antibodies to xenoantigens can lead to rapid antibody-mediated rejection (AMR) in xenotransplantation. We previously…
2018 American Transplant Congress
The Effect of Intravenous Immunoglobulin on In Vitro Human Antibody Binding to Genetically-Engineered Pig Cells – Relevance to Clinical Xenotransplantation –
T. Yamamoto,¹ Q. Li,¹ H. Iwase,¹ D. Ayares,² D. Cooper,¹ H. Hara.¹
¹Xenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, Birmingham; ²Revivicor, Blacksburg.
Background: Xenoreactive antibodies play an important role in xenograft rejection. Intravenous immunoglobulin (IVIG), which has anti-complement activity and contains anti-idiotypic antibodies, is widely used for…
2018 American Transplant Congress
Prevention of an Adaptive Immune Response by FDA-Approved Immunosuppressive Agents in Baboons with Pig Artery Patch Xenografts
T. Yamamoto,¹ H. Hara,¹ L. Wang,¹ Q. Li,¹ J. Li,¹ H. Zhou,¹ D. Eckhoff,¹ A. Tector,¹ D. Ayares,² D. Cooper,¹ H. Iwase.¹
¹Xenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, Birmingham; ²Revivicor, Blacksburg.
Background: In the pig-to-baboon artery patch model with no immunosuppressive therapy, a graft from a GTKO pig elicits a significant anti-nonGal IgG response, indicating sensitization…
2017 American Transplant Congress
A Swine for Human MHC Class I Swap: Implications for Xenotransplantation.
J. Ladowski,¹ R. Santos,² L. Reyes,¹ G. Martens,¹ J. Butler,² R. Sidner,¹ Z.-Y. Wang,¹ C. Cunningham,² M. Tector,¹ A. Tector.¹
¹Transplant Surgery, University of Alabama-Birmingham, Birmingham, AL; ²Surgery, Indiana University, Indianapolis, IN
Background: Xenotransplantation is a potential supply-side solution to the growing need for life-saving transplantable organs, but it was not until the recent advances in genetic…