2020 American Transplant Congress
In-Vivo Application of Ides (Imlifidase) in Nonhuman Primates Results in Xenoreactive Desensitization: A Novel Anti-Humoral Therapy
*Purpose: Antibody-mediated rejection is a formidable barrier to xenograft survival. A novel anti-humoral IdeS therapy, which cleaves serum IgG, represents a promising therapy to reduce…2020 American Transplant Congress
The Effect of B Cell Depletion Therapies on Preformed Xenoantibodies in Transplant Recipient
*Purpose: To assess the effect of B cell depletion therapies on preformed xenoantibodies in transplant recipient. Preformed xenoantibodies are a significant barrier to successful xenotransplantation.…2020 American Transplant Congress
Sensitization via Pregnancy Generates Alloantibody and Cross-Reactive Xeno-Antibody in a Non-Human Primate Model
Surgery, Duke University, Durham, NC
*Purpose: Women are disproportionately represented among highly sensitized waitlist candidates and within HLA-incompatible transplantation programs. Due to difficulties obtaining compatible donors, sensitized patients are seen…2020 American Transplant Congress
Xenoantibody Depletion of Human Blood with Normothermic Machine Perfusion of Porcine Kidney and Lung Prior to Liver Perfusion
*Purpose: Genetic modifications of porcine donors for pig-to-human xenotransplantation have led to significant progress in survival of xenografts in preclinical models. Triple knock-out (TKO) of…2020 American Transplant Congress
Old World Monkeys Are Not Ideal Transplantation Models for Testing CMAHKO (TKO or GTKO/CMAHKO) Pig Kidneys
1University of Alabama at Birmingham, Birmingham, AL, 2Revivicor, Blacksburg, VA
*Purpose: Triple-knockout (TKO) pigs are likely to be an optimal source of organs for transplantation into human recipients, many of whom do not have natural…2020 American Transplant Congress
Normothermic Machine Perfusion of Genetically Modified Pig Livers with Human Blood
*Purpose: Barriers to pig-to-human liver xenotransplantation remain, including profound thrombocytopenia, coagulopathy, and rejection. We utilized ex-vivo liver xenoperfusion with human blood to study the human…2019 American Transplant Congress
Newly-Revealed Antibody Targets Lead to the Early Loss of Triple Knockout Pig Kidneys in a Pig to Baboon Model
*Purpose: We have achieved >6 months survival of a life-supporting kidney with a vascularized thymus (VT) in a pig-to-baboon model. While improved survival has been…2019 American Transplant Congress
Kidney Xenotransplantation from Genetically Engineered Pigs to Baboons with FDA-Approved Immunosuppressive Therapy
*Purpose: Genetically-engineered pigs might present a solution to the critical shortage of organs for clinical transplantation. The aims of this study were to evaluate the…2019 American Transplant Congress
Utilizing Ides To Eliminate Residual Antibodies Targeting Xenografts
Massachusetts General Hospital, Boston, MA
*Purpose: A significant hinderance to the successful use of xenotransplantation has been the presence of preformed antibodies against the donor antigens, known as xenoantigens. Using…2019 American Transplant Congress
Islet Xenotransplantation: A Quest for Clinically Available Immunosuppression Regimens
1Surgery, Duke University, Durham, NC, 2University of Massachusetts, Worcester, MA
*Purpose: Porcine islet xenotransplantation has progressed significantly in non-human primate (NHP) studies. However, all successful NHP studies have relied upon immunosuppressive regimens that utilize drugs…