Articles tagged "Xenoreactive antibodies"

2020 American Transplant Congress
Impact of Desensitization Strategies with Proteosome Inhibition and Costimulation Blockade on Xenoreactive Humoral Responses
B. P. Lovasik¹, A. J. Matar¹, D. A. Faber¹, D. V. Mathews¹, C. P. Breeden¹, S. C. Kim¹, A. J. Tector², A. B. Adams¹
¹Emory University School of Medicine, Atlanta, GA, ²University of Miami, Miami, FL
*Purpose: “Delayed” antibody-mediated xenograft rejection is the one of the most important obstacles to clinical application of pig organ xenografts. The aim of this study…
2020 American Transplant Congress
Novel Technique to Analyze Blood-Endothelial Cell Interactions Under Flow Conditions
M. R. Connolly¹, K. Petitpas¹, L. Burdorf¹, B. Cerel¹, C. Laird², D. Harris², A. Azimzadeh¹, R. N. Pierson III¹
¹Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, ²University of Maryland, Baltimore, Baltimore, MD
*Purpose: The Bioflux system has been used to study platelet-driven thrombosis and leukocyte adhesion. Typically, whole blood is stained with calcein (green) to evaluate platelet…
2020 American Transplant Congress
Transient Cd4+ T Cell and Cd20+ B Cell Depletion Leads to Prolonged Pig-To-Primate Kidney Xenograft Survival
B. P. Lovasik¹, A. J. Matar¹, D. A. Faber¹, D. V. Mathews¹, C. Breeden¹, S. C. Kim¹, A. J. Tector², A. B. Adams¹
¹Emory University School of Medicine, Atlanta, GA, ²University of Miami, Miami, FL
*Purpose: “Delayed” antibody-mediated xenograft rejection is the one of the most important obstacle to clinical application of pig organ xenografts. The aim of this study…
2020 American Transplant Congress
Overcoming Ab-Mediated Xenolung Rejection: Beta4Gal is an Important Pre- and Post-Transplant Ab Target, Relevant to Xenograft Injury in Baboons
L. Burdorf¹, Z. Abady¹, B. Cerel¹, K. Petitpas¹, C. Laird², S. Pratts¹, X. Cheng², C. J. Phelps³, W. Eyestone³, D. L. Ayares³, R. N. Pierson III¹, A. M. Azimzadeh¹
¹Surgery, Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, ²Surgery, University of Maryland, Baltimore, MD, ³Revivicor, Blacksburg, VA
*Purpose: Knock-out (KO) of porcine carbohydrates (CHO), including Gal 1,3αGal, inhibits antibody-mediated xenograft rejection. The β4Gal epitope was discovered using serum from xenograft-sensitized baboons, but…
2020 American Transplant Congress
In-Vivo Application of Ides (Imlifidase) in Nonhuman Primates Results in Xenoreactive Desensitization: A Novel Anti-Humoral Therapy
B. P. Lovasik¹, A. J. Matar¹, D. A. Faber¹, D. V. Mathews¹, C. Breeden¹, S. C. Kim¹, A. J. Tector², A. Robertson³, C. Kjellman³, A. B. Adams¹
¹Emory University School of Medicine, Atlanta, GA, ²University of Miami, Miami, FL, ³HANSA Biopharma, Lund, Sweden
*Purpose: Antibody-mediated rejection is a formidable barrier to xenograft survival. A novel anti-humoral IdeS therapy, which cleaves serum IgG, represents a promising therapy to reduce…
2020 American Transplant Congress
The Effect of B Cell Depletion Therapies on Preformed Xenoantibodies in Transplant Recipient
S. Yeh¹, N. A. Wilson², A. Djamali², R. Redfield³
¹Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, ²Medicine/Nephrology, University of Wisconsin-Madison, Madison, WI, ³Surgery, University of Wisconsin-Madison, Madison, WI
*Purpose: To assess the effect of B cell depletion therapies on preformed xenoantibodies in transplant recipient. Preformed xenoantibodies are a significant barrier to successful xenotransplantation.…
2019 American Transplant Congress
Anti- CD154 / CD40 Costimulation Blockade is Superior to Tacrolimus in Prolonging Survival in Pig-to-Nonhuman Primate Renal Xenotransplantation
B. P. Lovasik¹, A. J. Matar¹, C. Breeden¹, J. Jenkins², J. Tector³, S. C. Kim¹, A. B. Adams¹
¹Emory Transplant Center, Emory University, Atlanta, GA, ²Yerkes National Primate Center, Emory University, Atlanta, GA, ³University of Alabama at Birmingham, Birmingham, AL
*Purpose: Costimulation blockade strategies targeting the CD40/CD154 pathway are highly effective in preventing xenograft rejection in pig-to-nonhuman primate (NHP) transplantation models. The aim of this…
2019 American Transplant Congress
Newly-Revealed Antibody Targets Lead to the Early Loss of Triple Knockout Pig Kidneys in a Pig to Baboon Model
Y. Ariyoshi¹, K. Takeuchi¹, T. Pomposelli¹, D. K. Ekanayake-Alper¹, C. Schuetz¹, H. Sahara¹, L. Boyd¹, E. Estime¹, S. Arn¹, D. Ayares², M. Lorber³, M. Sykes¹, D. Sachs¹, K. Yamada¹
¹CCTI/Surgery, Columbia University Medical Center, New York, NY, ²Revivicor Inc., Blacksburg, VA, ³Lung BioTechnology PBC, Silver Spring, MD
*Purpose: We have achieved >6 months survival of a life-supporting kidney with a vascularized thymus (VT) in a pig-to-baboon model. While improved survival has been…
2019 American Transplant Congress
Kidney Xenotransplantation from Genetically Engineered Pigs to Baboons with FDA-Approved Immunosuppressive Therapy
T. Yamamoto¹, H. Hara¹, J. Foote¹, E. C. Klein², H. J. Schuurman³, L. Wang¹, Q. Li¹, H. Zhou¹, J. Li¹, J. A. Tector¹, Z. Zhang², M. Ezzelarab², R. Lovingood¹, D. Ayares⁴, D. E. Eckhoff¹, D. K. Cooper¹, H. Iwase¹
¹University of Alabama at Birmingham, Birmingham, AL, ²University of Pittsburgh, Pittsburgh, PA, ³Schubiomed Consultancy, Utrecht, Netherlands, ⁴Revivicor, Blacksburg, VA
*Purpose: Genetically-engineered pigs might present a solution to the critical shortage of organs for clinical transplantation. The aims of this study were to evaluate the…
2019 American Transplant Congress
Utilizing Ides To Eliminate Residual Antibodies Targeting Xenografts
C. Rickert, K. Lee, N. Tanimine, J. Lei, A. Cuenca, N. Feeney, K. Deng, H. Yeh, J. F. Markmann
Massachusetts General Hospital, Boston, MA
*Purpose: A significant hinderance to the successful use of xenotransplantation has been the presence of preformed antibodies against the donor antigens, known as xenoantigens. Using…