2020 American Transplant Congress
Old World Monkeys Are Not Ideal Transplantation Models for Testing CMAHKO (TKO or GTKO/CMAHKO) Pig Kidneys
1University of Alabama at Birmingham, Birmingham, AL, 2Revivicor, Blacksburg, VA
*Purpose: Triple-knockout (TKO) pigs are likely to be an optimal source of organs for transplantation into human recipients, many of whom do not have natural…2020 American Transplant Congress
Normothermic Machine Perfusion of Genetically Modified Pig Livers with Human Blood
*Purpose: Barriers to pig-to-human liver xenotransplantation remain, including profound thrombocytopenia, coagulopathy, and rejection. We utilized ex-vivo liver xenoperfusion with human blood to study the human…2020 American Transplant Congress
Pig-To-Baboon Liver Xenotransplantation Utilizing Genetically Modified Swine
1Massachusetts General Hospital, Boston, MA, 2eGenesis, Cambridge, MA
*Purpose: Pig-to-baboon liver xenotransplant (LXT) continues with significant hurdles, including thrombocytopenia, coagulation dysregulation and rejection with the longest reported survival of 29 days utilizing a…2020 American Transplant Congress
Successful Long-Term TMA- and Rejection- Free Survival of a Kidney Xenograft with Triple Xenoantigen Knockout Plus Insertion of Multiple Human Transgenes
1Surgery, MGH, Boston, MA, 2eGenesis Inc., Cambridge, MA
*Purpose: Recent advances in genome engineering technology allow for the generation of porcine organ donors with multiplex gene edits. Here we evaluated survival of kidneys…2020 American Transplant Congress
Impact of Desensitization Strategies with Proteosome Inhibition and Costimulation Blockade on Xenoreactive Humoral Responses
1Emory University School of Medicine, Atlanta, GA, 2University of Miami, Miami, FL
*Purpose: “Delayed” antibody-mediated xenograft rejection is the one of the most important obstacles to clinical application of pig organ xenografts. The aim of this study…2020 American Transplant Congress
Novel Technique to Analyze Blood-Endothelial Cell Interactions Under Flow Conditions
*Purpose: The Bioflux system has been used to study platelet-driven thrombosis and leukocyte adhesion. Typically, whole blood is stained with calcein (green) to evaluate platelet…2019 American Transplant Congress
Anti- CD154 / CD40 Costimulation Blockade is Superior to Tacrolimus in Prolonging Survival in Pig-to-Nonhuman Primate Renal Xenotransplantation
*Purpose: Costimulation blockade strategies targeting the CD40/CD154 pathway are highly effective in preventing xenograft rejection in pig-to-nonhuman primate (NHP) transplantation models. The aim of this…2019 American Transplant Congress
Newly-Revealed Antibody Targets Lead to the Early Loss of Triple Knockout Pig Kidneys in a Pig to Baboon Model
*Purpose: We have achieved >6 months survival of a life-supporting kidney with a vascularized thymus (VT) in a pig-to-baboon model. While improved survival has been…2019 American Transplant Congress
Kidney Xenotransplantation from Genetically Engineered Pigs to Baboons with FDA-Approved Immunosuppressive Therapy
*Purpose: Genetically-engineered pigs might present a solution to the critical shortage of organs for clinical transplantation. The aims of this study were to evaluate the…2019 American Transplant Congress
Utilizing Ides To Eliminate Residual Antibodies Targeting Xenografts
Massachusetts General Hospital, Boston, MA
*Purpose: A significant hinderance to the successful use of xenotransplantation has been the presence of preformed antibodies against the donor antigens, known as xenoantigens. Using…