Articles tagged "Xenoreactive antibodies"

2021 American Transplant Congress
Successful Long-Term TMA- and Rejection-Free Survival of a Kidney Xenograft With Triple Xenoantigen Knockout Plus Insertion of Multiple Human Transgenes
T. Hirose¹, D. Ma¹, G. Lassiter¹, H. Sasaki¹, I. Rosales², T. Coe¹, C. Rickert¹, R. Matheson¹, R. Colvin², W. Qin³, Y. Kan³, J. Layer³, K. Stiede³, K. Hall³, M. Youd³, W. Westlin³, M. Curtis³, J. F. Markmann¹, T. Kawai¹
¹Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, ²Pathology, Massachusetts General Hospital, Boston, MA, ³eGenesis Inc, Cambridge, MA
*Purpose: Pigs with deletion of 3 carbohydrate xenoantigens (triple knock-out, TKO) are expected to be optimal donors for human xenotransplantation. However, anti-porcine natural antibodies of…
2021 American Transplant Congress
Impact of Allosensitization on Xenotransplantation
D. Olaso¹, M. Manook¹, J. Yoon¹, Y. Bae¹, A. Barbas¹, A. Adams², S. Knechtle¹, J. Kwun¹
¹Department of Surgery, Duke Transplantation Center, Durham, NC, ²Department of Surgery, University of Minnesota, Minneapolis, MN
*Purpose: Sensitization to human leukocyte antigens (HLA) due to prior exposure to foreign antigens through events such as blood transfusions, previous kidney transplant, or pregnancy…
2021 American Transplant Congress
Anti-pig IgE and IgA Antibodies in Sera of (i) Naïve Primates and (ii) Non-human Primates Sensitized to Pig Xenografts
H. Hara, Q. Li, H. Iwase, T. Yamamoto, D. Ayares, D. K. Cooper
University of Alabama at Birmingham, Birmingham, AL
*Purpose: Anti-pig IgM and IgG antibodies in primates play an important role in xenograft rejection. The possible roles of anti-pig IgE and IgA have not…
2021 American Transplant Congress
Triple-Knockout Pig Red Blood Cells Are a Potential Alternative Source for Clinical Blood Transfusion
T. Yamamoto¹, M. H. Bikhet¹, H. Q. Nguyen¹, M. Javed¹, D. Ayares², H. Iwase¹, H. Hara¹, D. K. Cooper¹
¹Xenotransplantation Program, Surgery, The University of Alabama at Birmingham, Birmingham, AL, ²Revivicor, Blacksburg, VA
*Purpose: Triple-knockout (TKO) pig RBCs (pRBCs) may be an alternative source for clinical blood transfusion because almost humans have no preformed antibody to TKO pRBCs.…
2020 American Transplant Congress
Pig-To-Baboon Liver Xenotransplantation Utilizing Genetically Modified Swine
D. Detelich¹, C. G. Rickert¹, T. M. Coe¹, S. Kimura¹, I. Rosales¹, H. Deng¹, H. Yeh¹, W. Qin², Y. Kan², J. Layer², M. Youd², W. Westlin², L. Yang², J. F. Markmann¹
¹Massachusetts General Hospital, Boston, MA, ²eGenesis, Cambridge, MA
*Purpose: Pig-to-baboon liver xenotransplant (LXT) continues with significant hurdles, including thrombocytopenia, coagulation dysregulation and rejection with the longest reported survival of 29 days utilizing a…
2020 American Transplant Congress
Successful Long-Term TMA- and Rejection- Free Survival of a Kidney Xenograft with Triple Xenoantigen Knockout Plus Insertion of Multiple Human Transgenes
D. H. Ma¹, H. Sasaki¹, T. Hirose¹, I. Rosales¹, A. Dehnadi¹, R. Colvin¹, J. F. Markmann¹, W. Win², Y. Kan², J. Layer², M. Youd², W. Westlin², L. Yang², T. Kawai¹
¹Surgery, MGH, Boston, MA, ²eGenesis Inc., Cambridge, MA
*Purpose: Recent advances in genome engineering technology allow for the generation of porcine organ donors with multiplex gene edits. Here we evaluated survival of kidneys…
2020 American Transplant Congress
Impact of Desensitization Strategies with Proteosome Inhibition and Costimulation Blockade on Xenoreactive Humoral Responses
B. P. Lovasik¹, A. J. Matar¹, D. A. Faber¹, D. V. Mathews¹, C. P. Breeden¹, S. C. Kim¹, A. J. Tector², A. B. Adams¹
¹Emory University School of Medicine, Atlanta, GA, ²University of Miami, Miami, FL
*Purpose: “Delayed” antibody-mediated xenograft rejection is the one of the most important obstacles to clinical application of pig organ xenografts. The aim of this study…
2020 American Transplant Congress
Novel Technique to Analyze Blood-Endothelial Cell Interactions Under Flow Conditions
M. R. Connolly¹, K. Petitpas¹, L. Burdorf¹, B. Cerel¹, C. Laird², D. Harris², A. Azimzadeh¹, R. N. Pierson III¹
¹Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, ²University of Maryland, Baltimore, Baltimore, MD
*Purpose: The Bioflux system has been used to study platelet-driven thrombosis and leukocyte adhesion. Typically, whole blood is stained with calcein (green) to evaluate platelet…
2020 American Transplant Congress
Transient Cd4+ T Cell and Cd20+ B Cell Depletion Leads to Prolonged Pig-To-Primate Kidney Xenograft Survival
B. P. Lovasik¹, A. J. Matar¹, D. A. Faber¹, D. V. Mathews¹, C. Breeden¹, S. C. Kim¹, A. J. Tector², A. B. Adams¹
¹Emory University School of Medicine, Atlanta, GA, ²University of Miami, Miami, FL
*Purpose: “Delayed” antibody-mediated xenograft rejection is the one of the most important obstacle to clinical application of pig organ xenografts. The aim of this study…
2020 American Transplant Congress
Overcoming Ab-Mediated Xenolung Rejection: Beta4Gal is an Important Pre- and Post-Transplant Ab Target, Relevant to Xenograft Injury in Baboons
L. Burdorf¹, Z. Abady¹, B. Cerel¹, K. Petitpas¹, C. Laird², S. Pratts¹, X. Cheng², C. J. Phelps³, W. Eyestone³, D. L. Ayares³, R. N. Pierson III¹, A. M. Azimzadeh¹
¹Surgery, Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, ²Surgery, University of Maryland, Baltimore, MD, ³Revivicor, Blacksburg, VA
*Purpose: Knock-out (KO) of porcine carbohydrates (CHO), including Gal 1,3αGal, inhibits antibody-mediated xenograft rejection. The β4Gal epitope was discovered using serum from xenograft-sensitized baboons, but…