Articles tagged "Xenoreactive antibodies"

2021 American Transplant Congress
Successful Long-Term TMA- and Rejection-Free Survival of a Kidney Xenograft With Triple Xenoantigen Knockout Plus Insertion of Multiple Human Transgenes
T. Hirose¹, D. Ma¹, G. Lassiter¹, H. Sasaki¹, I. Rosales², T. Coe¹, C. Rickert¹, R. Matheson¹, R. Colvin², W. Qin³, Y. Kan³, J. Layer³, K. Stiede³, K. Hall³, M. Youd³, W. Westlin³, M. Curtis³, J. F. Markmann¹, T. Kawai¹
¹Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, ²Pathology, Massachusetts General Hospital, Boston, MA, ³eGenesis Inc, Cambridge, MA
*Purpose: Pigs with deletion of 3 carbohydrate xenoantigens (triple knock-out, TKO) are expected to be optimal donors for human xenotransplantation. However, anti-porcine natural antibodies of…
2021 American Transplant Congress
Impact of Allosensitization on Xenotransplantation
D. Olaso¹, M. Manook¹, J. Yoon¹, Y. Bae¹, A. Barbas¹, A. Adams², S. Knechtle¹, J. Kwun¹
¹Department of Surgery, Duke Transplantation Center, Durham, NC, ²Department of Surgery, University of Minnesota, Minneapolis, MN
*Purpose: Sensitization to human leukocyte antigens (HLA) due to prior exposure to foreign antigens through events such as blood transfusions, previous kidney transplant, or pregnancy…
2021 American Transplant Congress
Anti-pig IgE and IgA Antibodies in Sera of (i) Naïve Primates and (ii) Non-human Primates Sensitized to Pig Xenografts
H. Hara, Q. Li, H. Iwase, T. Yamamoto, D. Ayares, D. K. Cooper
University of Alabama at Birmingham, Birmingham, AL
*Purpose: Anti-pig IgM and IgG antibodies in primates play an important role in xenograft rejection. The possible roles of anti-pig IgE and IgA have not…
2021 American Transplant Congress
Triple-Knockout Pig Red Blood Cells Are a Potential Alternative Source for Clinical Blood Transfusion
T. Yamamoto¹, M. H. Bikhet¹, H. Q. Nguyen¹, M. Javed¹, D. Ayares², H. Iwase¹, H. Hara¹, D. K. Cooper¹
¹Xenotransplantation Program, Surgery, The University of Alabama at Birmingham, Birmingham, AL, ²Revivicor, Blacksburg, VA
*Purpose: Triple-knockout (TKO) pig RBCs (pRBCs) may be an alternative source for clinical blood transfusion because almost humans have no preformed antibody to TKO pRBCs.…
2020 American Transplant Congress
Sensitization via Pregnancy Generates Alloantibody and Cross-Reactive Xeno-Antibody in a Non-Human Primate Model
J. Yoon, M. Manook, Z. Fitch, R. Schmitz, A. Choi, A. M. Jackson, S. Knechtle, J. Kwun
Surgery, Duke University, Durham, NC
*Purpose: Women are disproportionately represented among highly sensitized waitlist candidates and within HLA-incompatible transplantation programs. Due to difficulties obtaining compatible donors, sensitized patients are seen…
2020 American Transplant Congress
Xenoantibody Depletion of Human Blood with Normothermic Machine Perfusion of Porcine Kidney and Lung Prior to Liver Perfusion
T. M. Coe¹, N. Serifis¹, S. Raigani¹, C. Carroll¹, C. G. Rickert¹, R. Matheson¹, D. Detelich², W. Qin³, Y. Kan³, J. Layer³, M. Youd³, W. Westlin³, L. Yang³, A. Azimzadeh¹, J. F. Markmann¹
¹Surgery, Massachusetts General Hospital, Boston, MA, ²Surgery, Tufts Medical Center, Boston, MA, ³eGenesis, Cambridge, MA
*Purpose: Genetic modifications of porcine donors for pig-to-human xenotransplantation have led to significant progress in survival of xenografts in preclinical models. Triple knock-out (TKO) of…
2020 American Transplant Congress
Old World Monkeys Are Not Ideal Transplantation Models for Testing CMAHKO (TKO or GTKO/CMAHKO) Pig Kidneys
T. Yamamoto¹, H. Iwase¹, D. Patel¹, A. Jagdale¹, J. Foote¹, D. Ayares², D. Anderson¹, D. E. Eckhoff¹, D. K. Cooper¹, H. Hara¹
¹University of Alabama at Birmingham, Birmingham, AL, ²Revivicor, Blacksburg, VA
*Purpose: Triple-knockout (TKO) pigs are likely to be an optimal source of organs for transplantation into human recipients, many of whom do not have natural…
2020 American Transplant Congress
Normothermic Machine Perfusion of Genetically Modified Pig Livers with Human Blood
D. Detelich¹, T. M. Coe², C. G. Rickert², C. Carroll², N. Serifis², R. Matheson², I. Rosales², W. Qin³, Y. Kan³, J. Layer³, M. Youd³, W. Westlin³, S. Kimura², A. Azimzadeh², L. Yang³, J. F. Markmann²
¹Tufts Medical Center, Boston, MA, ²Massachusetts General Hospital, Boston, MA, ³eGenesis, Cambridge, MA
*Purpose: Barriers to pig-to-human liver xenotransplantation remain, including profound thrombocytopenia, coagulopathy, and rejection. We utilized ex-vivo liver xenoperfusion with human blood to study the human…
2020 American Transplant Congress
Pig-To-Baboon Liver Xenotransplantation Utilizing Genetically Modified Swine
D. Detelich¹, C. G. Rickert¹, T. M. Coe¹, S. Kimura¹, I. Rosales¹, H. Deng¹, H. Yeh¹, W. Qin², Y. Kan², J. Layer², M. Youd², W. Westlin², L. Yang², J. F. Markmann¹
¹Massachusetts General Hospital, Boston, MA, ²eGenesis, Cambridge, MA
*Purpose: Pig-to-baboon liver xenotransplant (LXT) continues with significant hurdles, including thrombocytopenia, coagulation dysregulation and rejection with the longest reported survival of 29 days utilizing a…
2020 American Transplant Congress
Successful Long-Term TMA- and Rejection- Free Survival of a Kidney Xenograft with Triple Xenoantigen Knockout Plus Insertion of Multiple Human Transgenes
D. H. Ma¹, H. Sasaki¹, T. Hirose¹, I. Rosales¹, A. Dehnadi¹, R. Colvin¹, J. F. Markmann¹, W. Win², Y. Kan², J. Layer², M. Youd², W. Westlin², L. Yang², T. Kawai¹
¹Surgery, MGH, Boston, MA, ²eGenesis Inc., Cambridge, MA
*Purpose: Recent advances in genome engineering technology allow for the generation of porcine organ donors with multiplex gene edits. Here we evaluated survival of kidneys…