Articles tagged "Tolerance"

2020 American Transplant Congress
Single Cell RNA Sequencing Analysis of 3 Human Living-Donor Liver Transplant Allograft Biopsies after Infusion of Donor-Derived Regulatory Dendritic Cells
L. M. Tran¹, T. Tabib², A. F. Zahorchak¹, R. Lafyatis², M. A. Styn¹, A. Humar¹, F. G. Lakkis¹, D. M. Metes¹, A. W. Thomson¹
¹Department of Surgery, University of Pittsburgh School of Medicine, Pittsburgh, PA, ²Department of Medicine and Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA
*Purpose: Single-cell RNA sequencing is an emerging, powerful genomic technology that can describe heterogenous cellular populations of whole solid organs with high resolution. We report…
2020 American Transplant Congress
Pregnancy Alloimmunization Increases the Clonality of the Endogenous Treg Repertoire
K. Krupp, E. Lewis, R. Xu, P. Porrett
University of Pennsylvania, Philadelphia, PA
*Purpose: Pregnancy is a common immune sensitizing event, which creates an access barrier to organ transplantation and can impact transplant outcomes. While most research has…
2020 American Transplant Congress
Recruitment of Regulatory T Cells Promotes Donor-Specific Tolerance in Vascularized Composite Allotransplantation
J. D. Fisher¹, W. Zhang², S. C. Balmert³, A. M. Aral¹, Y. Kulahci⁴, A. P. Acharya⁴, J. Li², H. R. Turnquist⁵, A. W. Thomson⁵, M. G. Solari², V. S. Gorantla⁶, S. R. Little⁴
¹Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, ²Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA, ³Department of Dermatology, University of Pittsburgh, Pittsburgh, PA, ⁴Department of Chemical Engineering, University of Pittsburgh, Pittsburgh, PA, ⁵Thomas E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA, ⁶Department of Bioengineering, Wake Forest University, Winston-Salem, NC
*Purpose: Vascularized composite allotransplantation (VCA) encompasses face and limb transplantation, but as with organ transplantation, it requires lifelong regimens of immunosuppressive drugs to prevent rejection.*Methods:…
2020 American Transplant Congress
Establishment of Spontaneous Tolerance of Kidney Allografts is Dependent on Mitigation of Ischemia-Reperfusion Injury via the BLTA-HVEM Pathway
Q. Yuan¹, S. Hong¹, E. Szuter¹, Y. Zhao¹, E. Acheampong¹, I. Rosales¹, M. Kronenberg², P. Russell¹, J. Madsen¹, R. Colvin¹, A. Alessandrini¹
¹Massachusetts General Hospital, Boston, MA, ²La Jolla Institute for Allergy & Immunology, La Jolla, CA
*Purpose: Kidney allografts transplanted across certain mouse strain combinations (e.g. DBA/2 to B6) develop tolerance while other combinations do not (e.g. B6 to DBA/2). We…
2020 American Transplant Congress
Granulocyte-Colony Stimulating Factor (G-CSF) and Granulocyte-Macrophage CSF (GM-CSF) Administration Enriches for Highly Suppressive CD4⁺CD45RA^–Foxp3^hi Cells in Leukapheresis Products of Rhesus Monkeys
K. Sasaki, Y. Wang, L. Lu, J. Hughes, V. Vujevich, A. W. Thomson, M. B. Ezzelarab
Starzl Transplantation Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA
*Purpose: In humans, CD4+CD45RA-Foxp3hi T cells comprise a regulatory T cell (Treg) subset with highly suppressive function. We have previously shown that granulocyte-colony stimulating factor…
2020 American Transplant Congress
Detrimental Effects of Donor Brain Death on Tolerance Induction May be Eliminated by Delaying Mixed Chimerism in Non-Human Primates
J. M. O¹, W. Sommer², K. Ahrens¹, P. Patel¹, D. Becerra¹, J. Morrissette¹, T. Costa¹, K. Pruner¹, J. Paster¹, A. Bean¹, A. Dehnadi¹, I. Hanekamp¹, I. Rosales¹, R. Smith¹, R. Colvin¹, G. Benichou¹, J. Allan³, T. Kawai¹, J. Madsen⁴
¹Center for Transplantation Sciences, MGH, Boston, MA, ²Department for Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany, ³Division of Thoracic Surgery, MGH, Boston, MA, ⁴Division of Cardiac Surgery, MGH, Boston, MA
*Purpose: Induction of tolerance to heart allografts in non-human primates is possible with kidney cotransplantation and mixed chimerism-based induction therapy. However, donor brain death abrogates…
2020 American Transplant Congress
Autophagy-Lysosome Inhibitor Chloroquine Prevents CTLA-4 Degradation of T Cells and Attenuates Acute Rejection in Murine Skin and Heart Transplantation
J. Wu
Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
*Purpose: The immune checkpoint cytotoxic T lymphocyte antigen-4 (CTLA-4), which was induced upon T cell activation but degraded quickly, has been targeted in the clinical…
2020 American Transplant Congress
Orthogonal IL-2 Cytokine-Receptor Pair Capable of Selective Expansion of Regulatory T Cells Facilitates Alloengraftment
T. Hirai¹, F. Simonetta², L. L. Su³, L. Picton³, J. Baker², J. Lin⁴, P. Li⁴, W. J. Leonard⁴, K. C. Garcia³, R. S. Negrin²
¹Department of Urology, Tokyo Women's Medical University, Tokyo, Japan, ²Division of Blood and Marrow Transplantation, Stanford University, Stanford, CA, ³Departments of Molecular and Cellular Physiology and Structural Biology, Stanford University, Stanford, CA, ⁴Laboratory of Molecular Immunology, National Institutes of Health, Bethesda, MD
*Purpose: Adoptive transfer of regulatory T cells (Tregs) has been shown to improve alloengraftment in animal models. However, ex vivo Treg expansion for the purpose…
2020 American Transplant Congress
Autologous Expanded Tregs Promote Bone Marrow Engraftment and Donor Kidney Survival in Non-Human Primate Chimerism Model
K. Bruestle¹, P. Alonso-Guallart¹, H. Sakai¹, F. Fredriksson¹, B. Piegari¹, E. Lopes¹, G. Pierre¹, A. Shou¹, D. Ekanayake-Alper¹, J. Weiner¹, A. Iuga², S. M. Coley², M. Sykes¹, A. Griesemer¹
¹Columbia Center for Translational Immunology, Columbia University, New York City, NY, ²Department of Pathology and Cell Biology, Columbia University, New York City, NY
*Purpose: Mixed chimerism (MC) induced with non-myeloablative conditioning in monkeys and humans induces tolerance to 70% of kidney grafts transplanted at the time of BMT.…
2020 American Transplant Congress
ESAT-6, A Novel Protein Originally Produced by M. Tuberculosis, Inhibits Central Memory CD8+ T Cell Generation and Allograft Rejection
F. Qiu, S. Ye, H. Liu, Z. Dai
Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou, China
*Purpose: Allospecific memory T cells pose a threat to long-term allograft survival since they are somewhat resistant to immunosuppression. The objective of this study is…