Articles tagged "T cell reactivity"

2020 American Transplant Congress
Neonatal Tolerance Induction: Behaviours of Allogeneic Spleen and Bone Marrow Inocula in Neonatal Mice are Largely Determined by the Nature of Their CD8 T Cells
R. A. Bascom, K. Tao, L. J. West
University of Alberta, Edmonton, AB, Canada
*Purpose: Injected adult allo-spleen (SC) and bone marrow cells (BMC) traffic to secondary lymphoid organs, proliferate and spread throughout neonatal mice; allo-SC consistently cause acute…
2020 American Transplant Congress
Non-Human Primate B Cells Into Regulatory B Cells by Activation with CpG
K. Lee¹, C. Rickert¹, K. Deng¹, N. Feeney¹, N. Tanimine², Q. Fu¹, G. Huai¹, J. Lei¹, C. Leguern¹, J. Markmann¹
¹Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, ²Gastroenterological Transplantation Surgery, Hiroshima University, Hiroshima, Japan
*Purpose: We previously generated Toll like receptor (TLR)-activated B cells in rodent settings. These B cells can suppress T cell proliferation via in vitro mixed…
2020 American Transplant Congress
Evaluating Preformed Donor HLA Reactive T Cells Using PIRCHE-II Algorithm
T. Tomosugi¹, K. Iwasaki², S. Sakamoto¹, A. Kanda¹, K. Futamura¹, M. Okada¹, T. Hiramitsu¹, N. Goto¹, S. Narumi¹, Y. Watarai¹, T. Kobayashi², M. Niemann³, E. Spierings⁴
¹Transplant and Endocrine Surgery, Nagoya Daini Red Cross Hospital, Nagoya, Aichi, Japan, ²Kidney Transplant Surgery, Aichi Medical University School of Medicine, Nagakute, Aichi, Japan, ³PIRCHE AG, Berlin, Germany, ⁴University Medical Center Utrecht, Utrecht, Netherlands
*Purpose: De novo donor-specific HLA antibodies (dnDSA) production involves the indirect allorecognition pathway in which recipient CD4+T cells recognize donor-HLA-derived peptide and recipient HLA class…
2020 American Transplant Congress
Autophagy-Lysosome Inhibitor Chloroquine Prevents CTLA-4 Degradation of T Cells and Attenuates Acute Rejection in Murine Skin and Heart Transplantation
J. Wu
Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
*Purpose: The immune checkpoint cytotoxic T lymphocyte antigen-4 (CTLA-4), which was induced upon T cell activation but degraded quickly, has been targeted in the clinical…
2019 American Transplant Congress
Microparticle Delivery to Lymph Nodes Induces Specific Suppression to Prolong Islet Survival
Y. Xiong¹, L. Tostanoski², C. Jewell³, J. Bromberg¹
¹Surgery, U. Maryland, Baltimore, MD, ²U.Maryland, Baltimore, MD, ³U. Maryland, College Park, MD
*Purpose: Microparticles (MP) loaded with peptide antigen plus other immune signals and delivered directly into lymph nodes (LN) can result in either proinflammatory or antiinflammatory…
2019 American Transplant Congress
Using a Commercially Available Assay That Measures Cytomegalovirus-Specific CD4+ and CD8+ T-cell Immunity to Predict Clinically Significant Cytomegalovirus Events in Solid Organ Transplant Recipients
R. Rogers¹, K. Saharia², A. Chandorkar², Z. Weiss³, D. Farmakiotis¹
¹Infectious Diseases, Brown University, Providence, RI, ²Infectious Diseases, University of Maryland, Baltimore, MD, ³Internal Medicine, Brown University, Providence, RI
*Purpose: To assess the diagnostic performance and clinical utility of the Viracor CMV T-cell Immunity Panel (CMV-TCIP), which measures both CMV-specific Cd4+ and Cd8+ T-cell…
2019 American Transplant Congress
Predicted Indirectly Recognizable H L A Epitopes Are Associated with T-cell Mediated Rejection among Kidney Transplant Recipients with Preformed and De Novo Donor-Reactive T-cells
T. Schachtner¹, M. Stein², N. M. Otto³, P. M. Reinke²
¹Nephrology, University Hospital Zurich, Zurich, Switzerland, ²Berlin-Brandenburg Center for Regenerative Therapies, Charite University Medicine Berlin, Berlin, Germany, ³Berlin-Brandenburg Center for Regenerative Therapies, Charite University Medicine Berlin, Berin, Germany
*Purpose: Very recently Predicted Indirectly ReCognizable HLA Epitopes (PIRCHE) have been suggested to demonstrate early and late effects on kidney allograft outcomes. The association between…
2019 American Transplant Congress
Regulating T Cell Responses by Forced Transgenic Expression of Decay-Accelerating Factor
D. Heja¹, Z. He¹, S. A. Lira¹, F. Lin², D. Homann¹, P. S. Heeger¹
¹Icahn School of Medicine at Mount Sinai, New York, NY, ²Cleveland Clinic, Cleveland, OH
*Purpose: Decay-accelerating factor (DAF, CD55) is a cell surface expressed, glycophosphatidylinositol-(GPI)-anchored, complement regulator. Previous studies showed that during cognate T cell/antigen presenting cell (APC) interactions…
2019 American Transplant Congress
First Report and Mechanistic Analysis of Operational Tolerance after Intestinal Transplant
J. Weiner¹, S. Moturi¹, C. Ashokkumar², K. Khan¹, S. Kaufman¹, R. Sindhi², C. Matsumoto¹, T. Fishbein¹, A. Kroemer¹
¹MedStar Georgetown Transplant Institute, Washington, DC, ²University of Pittsburgh, Pittsburgh, PA
*Purpose: Transplantation tolerance is a highly sought and rarely achieved state in clinical medicine. Defined as long-term stable and acceptable graft tolerance without immunosuppression and…
2019 American Transplant Congress
MDSCs Prolong Allogeneic Cardiac Graft Survival by Regulating Interferon Type 1 Signaling
S. Cai¹, P. T. Sage¹, T. J. Borges¹, T. Ichimura¹, S. K. Eskandari¹, J. Y. Choi¹, I. Salkuj¹, B. S. AI Dulaijan¹, H. Allos¹, J. Miao², L. V. Riella¹, A. K. Chandraker¹, J. R. Azzi¹
¹Brigham and Women's Hospital, Harvard Medical School, Boston, MA, ²Boston Children's Hospital, Harvard Medical School, Boston, MA
*Purpose: We previously demonstrated that donor-derived regulatory dendritic cells (DCreg), contrary to recipient derived DCreg, promote antigen-specific allograft protection. As myeloid-derived suppressor cells (MDSC) show…