Articles tagged "Primates"

2020 American Transplant Congress
Peak DSA Levels Pre-Transplant but Not at Transplant Predict Graft Survival
A. Y. Choi¹, D. Olaso¹, T. Risoli Jr², M. Kuchibhatla², Z. Fitch³, R. Schmitz³, P. M. Schroder³, A. Jackson³, M. Manook³, J. Yoon³, A. B. Farris⁴, J. Kwun³, S. J. Knechtle³
¹Duke University School of Medicine, Durham, NC, ²Duke Biostatistics, Epidemiology, and Research Design Core, Duke University, Durham, NC, ³Department of Surgery, Duke University Medical Center, Durham, NC, ⁴Department of Pathology, Emory University Hospital, Atlanta, GA
*Purpose: Immunization to major histocompatibility complex (MHC) antigens is a barrier in kidney transplantation (KTx) due to higher risk of antibody-mediated rejection (AMR) and graft…
2020 American Transplant Congress
IL-6 Receptor Blockade But Not IL-2 Treatment Contributes to Long-Term NHP Cardiac Allograft Survival in Transient Mixed Chimeras
K. Ahrens¹, J. M. O¹, W. Sommer², J. Morrissette¹, D. Becerra¹, P. M. Patel¹, T. Costa¹, A. Dehnadi¹, I. M. Hanekamp¹, J. S. Allan³, G. Benichou¹, J. C. Madsen⁴
¹Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, ²Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany, ³Center for Transplantation Sciences, Division of Thoracic Surgery, Massachusetts General Hospital, Boston, MA, ⁴Center for Transplantation Sciences, Division of Cardiac Surgery, Massachusetts General Hospital, Boston, MA
*Purpose: Long-term tolerance of cardiac allografts has been achieved in non-human primates (NHPs) using a mixed chimerism protocol and by co-transplanting a kidney from the…
2020 American Transplant Congress
Old World Monkeys Are Not Ideal Transplantation Models for Testing CMAHKO (TKO or GTKO/CMAHKO) Pig Kidneys
T. Yamamoto¹, H. Iwase¹, D. Patel¹, A. Jagdale¹, J. Foote¹, D. Ayares², D. Anderson¹, D. E. Eckhoff¹, D. K. Cooper¹, H. Hara¹
¹University of Alabama at Birmingham, Birmingham, AL, ²Revivicor, Blacksburg, VA
*Purpose: Triple-knockout (TKO) pigs are likely to be an optimal source of organs for transplantation into human recipients, many of whom do not have natural…
2020 American Transplant Congress
Splenectomy Impedes B Cell Infiltration Associated with Rejection of Neonatal Porcine Islet Xenografts
Z. W. Fitch, M. Song, R. Schmitz, F. Leopardi, M. Ribeiro, A. Miller, G. Devi, B. Collins, X. Luo, A. D. Kirk
Duke University, Durham, NC
*Purpose: Xenotransplantation has historically been frustrated by antibody-mediated rejection. Splenectomy has shown efficacy in mediating humoral immunity. We hypothesized that adding splenectomy to a clinically…
2020 American Transplant Congress
Expression of Multiple Human Complement Regulatory Proteins Results in Prolonged Heterotopic Xenoheart Survival in an NHP Model
K. Ahrens¹, J. M. O¹, W. Sommer², D. Becerra¹, P. M. Patel¹, A. Dehnadi¹, Y. Kan³, J. Layer³, J. Morrissette¹, T. Costa¹, I. M. Hanekamp¹, J. S. Allan⁴, M. Youd³, R. N. Pierson⁵, W. Qin³, L. Burdorf⁵, A. M. Azimzadeh¹, W. Westlin³, L. Yang³, J. C. Madsen⁵
¹Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, ²Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany, ³eGenesis Inc., Cambridge, MA, ⁴Center for Transplantation Sciences, Division of Thoracic Surgery, Massachusetts General Hospital, Boston, MA, ⁵Center for Transplantation Sciences, Division of Cardiac Surgery, Massachusetts General Hospital, Boston, MA
*Purpose: Advances in genome engineering technology allow for multiplex genetic modifications in pigs designed to address immunologic and coagulation incompatibilities in order to facilitate xenotransplantation.…
2020 American Transplant Congress
Pig-To-Baboon Liver Xenotransplantation Utilizing Genetically Modified Swine
D. Detelich¹, C. G. Rickert¹, T. M. Coe¹, S. Kimura¹, I. Rosales¹, H. Deng¹, H. Yeh¹, W. Qin², Y. Kan², J. Layer², M. Youd², W. Westlin², L. Yang², J. F. Markmann¹
¹Massachusetts General Hospital, Boston, MA, ²eGenesis, Cambridge, MA
*Purpose: Pig-to-baboon liver xenotransplant (LXT) continues with significant hurdles, including thrombocytopenia, coagulation dysregulation and rejection with the longest reported survival of 29 days utilizing a…
2020 American Transplant Congress
Successful Long-Term TMA- and Rejection- Free Survival of a Kidney Xenograft with Triple Xenoantigen Knockout Plus Insertion of Multiple Human Transgenes
D. H. Ma¹, H. Sasaki¹, T. Hirose¹, I. Rosales¹, A. Dehnadi¹, R. Colvin¹, J. F. Markmann¹, W. Win², Y. Kan², J. Layer², M. Youd², W. Westlin², L. Yang², T. Kawai¹
¹Surgery, MGH, Boston, MA, ²eGenesis Inc., Cambridge, MA
*Purpose: Recent advances in genome engineering technology allow for the generation of porcine organ donors with multiplex gene edits. Here we evaluated survival of kidneys…
2020 American Transplant Congress
The Effect of Human Thrombomodulin on Thrombotic Complications in Life Supporting Pig-to-Baboon Cardiac Xenotransplantation
C. E. Goerlich¹, A. Singh¹, D. Kaczorowski¹, M. Abdullah¹, B. Lewis¹, I. Tatarov¹, A. Hershfeld¹, T. Zhang¹, P. Odonkor¹, E. Strauss¹, B. Williams¹, D. Ayares², M. Mohiuddin¹
¹Surgery, University of Maryland School of Medicine, Baltimore, MD, ²Revivicor, Blacksburg, VA
*Purpose: Transplantation of organs from animals to humans (xenotransplantation) has been proposed to address the critical shortage of organs for transplantation. The use of genetically…
2020 American Transplant Congress
Human, Monkey Transplant Tolerance and IL35 “Exokine
W. J. Burlingham¹, E. Jankowska-Gan², D. Lema², J. H. Fechner², J. A. Sullivan², D. B. Kaufman²
¹University of Wisconsin, Madison, WI, ²Surgery, University of Wisconsin, Madison, WI
*Purpose: Studies in B6 (H2b) mice tolerized to CBA (H2k) have shown that induced allospecific Tregs make 2 very different forms of immunoregulatory cytokines: 1)…
2020 American Transplant Congress
Establishing an Induction Protocol for Long-Term Multilineage Mixed Chimerism in Rhesus Macaques
K. Bruestle¹, H. Sakai¹, F. Fredriksson¹, B. Piegari¹, D. Ekanayake-Alper¹, A. McLuckie², R. Ober², J. Weiner¹, A. Iuga³, S. M. Coley³, M. Sykes¹, A. Griesemer¹
¹Columbia Center for Translational Immunology, Columbia University, New York City, NY, ²Institute for Comparative Medicine, Columbia University, New York City, NY, ³Department of Pathology and Cell Biology, Columbia University, New York City, NY
*Purpose: Mixed chimerism (MC) induction via non-myeloablative conditioning and bone marrow transplantation (BMT) induces tolerance to solid organs. Our standard MC induction protocol in cynomologous…