Articles tagged "Primates"

2021 American Transplant Congress
First Full Reversal of Diabetes in Non-human Primates (nhp) with Stem Cell Derived Islet-like-clusters (sc-ilcs)
J. Lei¹, S. Collins², H. Deng¹, Z. Yang¹, A. Zhang¹, C. Peters¹, J. Zhang², K. Boulanger², M. Yu³, M. Rickels³, C. Liu³, R. Pop², A. Naji³, D. Melton², J. Markmann¹
¹Surgery, Mass General, Boston, MA, ²Harvard Stem Cell Institute, Harvard University, Boston, MA, ³Surgery, Upenn, Philadelphia, PA
*Purpose: Deceased donor allo-islet transplants (Tx) have demonstrated the ability of cell-based therapy to fully reverse T1D, however, an inadequate donor supply and frequent need…
2021 American Transplant Congress
P2X7 Receptor Activity Mediates Th17-Specific Self- and Alloreactivity in Non-Human Primates
C. Little, J. Sullivan, W. Burlingham, D. Kaufman
Department of Surgery, University of Wisconsin, Madison, WI
*Purpose: The broadly expressed ATP-binding purinergic receptor, P2X7 (P2X7R) is involved in the Th17 mediated cellular immune response to allo- and select self-antigens (Collagen type…
2021 American Transplant Congress
Effects of IL-2 and/ or Anti-IL6R Therapy on Long-term Cardiac Allograft Survival in Non-Human Primates (NHPs)
C. L. Miller, K. J. Ahrens, J. M. O, P. M. Patel, J. A. Morrissette, D. Becerra, T. Costa, A. Dehnadi, I. M. Hanekamp, G. Benichou, J. C. Madsen
Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA
*Purpose: Cardiac allograft tolerance in NHPs has been achieved by our group using a mixed chimerism model with allogeneic bone marrow transplant after non-myeloablative conditioning…
2021 American Transplant Congress
Initial Experience with Growth Hormone Receptor Knockout in Pig-to-Baboon Kidney Transplantation: Effect on Ureteric Viability and Kidney Growth
C. Hansen-Estruch¹, H. Iwase¹, A. Jagdale¹, T. Yamamoto¹, M. H. Bikhet¹, J. Foote², H. Hara¹, D. Anderson¹, P. Porrett¹, D. Eckhoff¹, D. Ayares³, W. Eyestone³, J. Locke¹, D. K. Cooper¹
¹Xenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, Birmingham, AL, ²Department of Microbiology and Animal Resources Program, University of Alabama at Birmingham, Birmingham, AL, ³Revivicor, Blacksburg, VA
*Purpose: The purpose of this study was to report outcomes of pig-to-baboon renal transplants using a novel growth hormone receptor knockout (GHRKO) organ-source pig created…
2020 American Transplant Congress
Granulocyte-Colony Stimulating Factor (G-CSF) and Granulocyte-Macrophage CSF (GM-CSF) Administration Enriches for Highly Suppressive CD4⁺CD45RA^–Foxp3^hi Cells in Leukapheresis Products of Rhesus Monkeys
K. Sasaki, Y. Wang, L. Lu, J. Hughes, V. Vujevich, A. W. Thomson, M. B. Ezzelarab
Starzl Transplantation Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA
*Purpose: In humans, CD4+CD45RA-Foxp3hi T cells comprise a regulatory T cell (Treg) subset with highly suppressive function. We have previously shown that granulocyte-colony stimulating factor…
2020 American Transplant Congress
Tracking Tregs in a Non-Human Primate Model with Expanded Autologous Treg Infusions In Vivo
K. Bruestle¹, B. Piegari¹, H. Sakai¹, F. Fredriksson¹, D. Ekanayake-Alper¹, S. Robertson², D. Hajosi², J. Weiner¹, A. Iuga³, S. M. Coley³, M. Sykes¹, A. Griesemer¹
¹Columbia Center for Translational Immunology, Columbia University, New York City, NY, ²Institute for Comparative Medicine, Columbia University, New York City, NY, ³Department of Pathology and Cell Biology, Columbia University, New York City, NY
*Purpose: Regulatory Tcell (Treg) therapy is a promising tool for tolerance induction in solid organ transplantation. Despite encouraging results in humans and monkeys, questions about…
2020 American Transplant Congress
Transient Cd4+ T Cell and Cd20+ B Cell Depletion Leads to Prolonged Pig-To-Primate Kidney Xenograft Survival
B. P. Lovasik¹, A. J. Matar¹, D. A. Faber¹, D. V. Mathews¹, C. Breeden¹, S. C. Kim¹, A. J. Tector², A. B. Adams¹
¹Emory University School of Medicine, Atlanta, GA, ²University of Miami, Miami, FL
*Purpose: “Delayed” antibody-mediated xenograft rejection is the one of the most important obstacle to clinical application of pig organ xenografts. The aim of this study…
2020 American Transplant Congress
Early Post-Transplant Infusion of Ex Vivo-Expanded Autologous Polyclonal Regulatory T Cells (Treg) Prolongs Kidney Allograft Survival in Non-Lymphodepleted, CTLA4Ig-Treated Rhesus Monkeys
K. Sasaki, Y. Wang, R. Nakano, L. Lu, J. Hughes, V. Vujevich, A. J. Ganoza, M. Wijkstrom, A. Humar, A. W. Thomson, M. B. Ezzelarab
Starzl Transplantation Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA
*Purpose: Rodent studies have demonstrated the utility of regulatory T cell (Treg) infusion for the promotion of tolerance to organ allografts. Here, we evaluated the…
2020 American Transplant Congress
Long-term Functional Survival of Human Stem Cell-Derived Islets Microencapsulated in Alginate with CXCL12 in Non-Human Primates without Immunosuppression
M. C. Poznansky¹, D. Alagpulinsa¹, J. Lei², R. Pop³, D. Sobell⁴, M. Chapin¹, S. Collins³, A. Veres³, H. Deng², D. Melton³, J. Markmann²
¹Vaccine and Immunotherapy Center, Massachusetts General Hospital, Charlestown Navy Yard, MA, ²Transplant Surgery, Massachusetts General Hospital, Boston, MA, ³Harvard Stem Cell Institute, Harvard University, Cambridge, MA, ⁴Vaccine and Immunotherapy Center, Massachusetts General Hospital, Boston, MA
*Purpose: Human pluripotent stem cell-derived islet-like cells (SC-islets) represent a practical source for beta-cell replacement to achieve a functional cure for type 1 diabetes. Long-term…
2020 American Transplant Congress
Donor Mesenchymal Stem Cells in Combination with Co-Stimulation Blockade Prolong Allograft Survival
A. J. Matar, B. P. Lovasik, S. C. Kim, C. A. Breeden, D. A. Faber, D. V. Mathews, J. Regenold, A. Ghosh, A. Stephenson, W. H. Kitchens, A. B. Adams
Surgery and Transplantation, Emory University, Atlanta, GA
*Purpose: Co-stimulation blockade (CoB) has emerged as a promising immunosuppression strategy and potential alternative to calcineurin inhibitors in solid organ transplantation. Despite recent advancements, CoB…