Articles tagged "Preservation"

2021 American Transplant Congress
The Harmful Effect of Retained Peripheral Cells During Flushing and Static Cold Storage in Rat Liver Normothermic Machine Perfusion
O. Haque¹, C. A. Pendexter², J. F. Markmann², K. Uygun², H. Yeh², S. N. Tessier²
¹Surgery, Beth Israel Deaconess Medical Center, Boston, MA, ²Massachusetts General Hospital, Boston, MA
*Purpose: Retained donor blood in a liver graft can cause damage to resident cells that is exacerbated by static cold storage (SCS), leading to platelet…
2021 American Transplant Congress
Combining Oxygenated Cold Perfusion with Normothermic Ex-vivo Perfusion Improves the Outcome of DCD Porcine Kidney Transplantation
L. I. Mazilescu¹, T. Goto¹, R. Rosales¹, Y. Noguchi¹, P. Urbanellis¹, L. A. Robinson², M. Selzner¹
¹Ajmera Transplant Program, Toronto General Hospital, Toronto, ON, Canada, ²Department of Nephrology, The Hospital for Sick Children, Toronto, ON, Canada
*Purpose: Ex-vivo machine perfusion is a novel preservation technique for the storage and assessment of marginal kidney grafts. Normothermic (NEVKP) as well as hypothermic machine…
2021 American Transplant Congress
Determining Endpoint Criteria in Ex Vivo Normothermic Limb Perfusion (EVNLP)
S. K. Pandey¹, A. Meyers¹, P. Sadeghi¹, V. Kopparthy¹, T. Xia¹, H. Brunengraber², S. Dasarathy³, A. Rampazzo¹, B. Bassiri Gharb¹
¹Plastic Surgery, Cleveland Clinic, Cleveland, OH, ²Department of Nutrition, School of Medicine, Case Western Reserve University, Cleveland, OH, ³Department of Gastroenterology, Cleveland Clinic, Cleveland, OH
*Purpose: There are no established criteria for discontinuing EVNLP before irreversible muscle and endothelial cell damage occur. This study aimed to evaluate weight gain as…
2021 American Transplant Congress
Efficacy of Ex Vivo Normothermic Limb Perfusion in Maintaining Cellular Viability and Muscle Function
T. Xia¹, P. Sadeghi², V. Kopparthy², S. Pandey², A. Rampazzo², B. Bassiri Gharb²
¹School of Medicine, Case Western Reserve University, Cleveland, OH, ²Department of Plastic Surgery, Cleveland Clinic, Cleveland, OH
*Purpose: The purpose of this study was to evaluate cellular injury and myocyte function of amputated limbs following ex vivo normothermic limb perfusion (EVNLP) compared…
2021 American Transplant Congress
The Edge of Glory: Determinants of Success and Failure for Prolonged Cold Ischemia in Kidney Transplantation
A. Turner¹, M. Hamidi¹, V. Ariyamuthu², R. Harland¹, B. Tanriover²
¹Surgery, Banner University of Arizona Medical Center, Tucson, AZ, ²Medicine, Banner University of Arizona Medical Center, Tucson, AZ
*Purpose: Prolonged cold ischemia time (CIT>30 hours) is associated with an increased risk of primary non-function and early graft loss in kidney transplantation, although this…
2021 American Transplant Congress
Ex Situ Heart Perfusion and Standard of Care Cold Storage Differentially Affect the Ischemic Secretome of Donor Hearts in Perfusates but Not the Reperfusion Response in Recipient Plasma
E. Chichelnitskiy¹, B. Wiegmann², N. Ledwoch¹, F. Ius², F. Wandrer¹, J. Kühne¹, K. Beushausen¹, J. Keil¹, S. V. Rojas², W. Sommer², C. Kühn², I. Tudorache², M. Avsar², A. Haverich², G. Warnecke², C. S. Falk¹
¹MHH, Institute of Transplant Immunology, Hannover, Germany, ²MHH, Department for Cardiothoracic, Transplantation and Vascular Surgery, Hannover, Germany
*Purpose: Various allograft preservation procedures influence the donor organ status that may be reflected by the cytokine/chemokine microenvironment in heart transplant (HTx) recipients. In addition,…
2021 American Transplant Congress
Combined Liver and Lung Transplantation with Extended Normothermic Liver Preservation Using Transmedics Organ Care System (OCS)^TM Liver: A Single Center Experience
J. Konel¹, M. Shamaa², O. Shamaa², A. Elsabbagh², T. Kitajima², T. Ivanics², K. Delvecchio², A. Mohamed², S. Yeddula², K. Collins², A. Yoshida², M. Abouljoud², S. Nagai², M. Rizzari²
¹Wayne State University School of Medicine, Detroit, MI, ²Henry Ford Transplant Institute, Detroit, MI
*Purpose: Combined liver-lung transplantation (CLLT) is indicated in patients who cannot survive single-organ transplantation alone. Ex-situ normothermic machine perfusion (NMP) has been used to increase…
2021 American Transplant Congress
Mitigating the Adverse Impact of Broader Kidney Sharing with Hospital-based Machine Perfusion
Y. Yushkov, B. R. Schleich, T. Carrea, V. Wadhera, M. J. Goldstein
Hackensack University Medical Center, Hackensack, NJ
*Purpose: The new UNOS kidney allocation changes effective December 2020 will be removing the geographic limits of donation service areas (DSA) and UNOS regions favoring…
2021 American Transplant Congress
Recovery After Extended Static Cold Storage Preservation Using Subnormothermic Machine Perfusion in VCA
M. Goutard¹, R. J. de Vries¹, A. G. Lellouch¹, E. Lupon¹, C. Pendexter¹, S. N. Tessier¹, M. A. Randolph¹, L. Lantieri², C. L. Cetrulo¹, K. Uygun¹
¹Massachusetts General Hospital, Harvard Medical School, Shriners Hospitals for Children, Boston, MA, ²Hôpital Européen Georges Pompidou, Paris, France
*Purpose: Vascularized composite allografts (VCA) are more sensitive to cold ischemia than solid organ transplants due to the remaining metabolism in skeletal muscle cells. In…
2021 American Transplant Congress
Segmental Susceptibility of Intestinal Stem Cells to Cold Storage Preservation Injury in a Porcine Model
E. K. Ludwig¹, N. Abraham², D. Sudan², A. Barbas², C. Schaaf¹, J. Freund¹, A. S. Stewart¹, B. Veerasammy¹, L. M. Gonzalez¹
¹North Carolina State University, Raleigh, NC, ²Duke University, Durham, NC
*Purpose: Cold storage (CS) is the current gold standard preservation method for small bowel (SB) prior to transplantation. However, CS can cause epithelial detachment, sloughing,…