Articles tagged "Preservation"

2021 American Transplant Congress
Superior Post-transplant Clinical Outcomes Using Portable Normothermic Perfusion and Assessment with the Organ Care System (ocs) Liver System: 1-year Outcomes of the Ocs Liver Protect Randomized Controlled Trial
J. Markmann¹, M. Abouljoud², M. Ghobrial³, C. Bhati⁴, S. Pelletier⁵, J. Magliocca⁶, T. Pruett⁷, A. Lu⁸, M. Rizzari⁹, S. Ottmann¹⁰, T. Klair¹¹, C. Eymard¹², G. Roll¹³, G. Reyes¹⁴, S. Black¹⁵, S. Florman¹⁶, S. Mirani¹⁷, C. Marsh¹⁸, G. Schnickel¹⁹, M. Kinkhabwala²⁰, A. Demetris²¹, H. Yeh²², P. Vagefi²³, M. MacConmara²³
¹MGH, Boston, MA, ²Henry Ford, Detroit, MI, ³Houston Methodist, Houston, TX, ⁴Virginia Commonwealth, Richmond, VA, ⁵UVA, Charlottesville, VA, ⁶Emory, Atlanta, GA, ⁷U. Minnesota, Minneapolis, MN, ⁸Tampa General, Tampa, FL, ⁹Henry Ford, Detriot, MI, ¹⁰Johns Hopkins, Baltimore, MD, ¹¹University of Texas HSC, San Antonio, TX, ¹²University of Tennessee HSC, Memphis, TN, ¹³UCSF, San Francisco, CA, ¹⁴U. Washington, Seattle, WA, ¹⁵Ohio State, Columbus, OH, ¹⁶Mt. Sinai, New York, NY, ¹⁷U. Nebraska, Omaha, NE, ¹⁸Scripps Clinic, San Diego, CA, ¹⁹U. San Diego, San Diego, CA, ²⁰Montefiore, New York, MA, ²¹UPMC, Pittsburgh, PA, ²²Massachusetts General Hospital, Boston, MA, ²³UT Southwestern, Dallas, TX
*Purpose: The OCS Liver PROTECT Trial was the first U.S. randomized controlled trial for liver perfusion and was designed to evaluate the impact of portable…
2021 American Transplant Congress
Cross-Circulation for Extracorporeal Liver Support in a Swine Model
W. K. Wu¹, A. Tumen¹, J. W. Stokes², R. Ukita², A. E. Hozain³, C. R. Flynn², M. J. Lee³, J. R. Talackine², N. L. Cardwell², J. A. Reimer³, M. Pinezich³, C. Benson², G. Vunjak-Novakovic³, S. P. Alexopoulos², M. Bacchetta²
¹co-first authors, Vanderbilt University Medical Center, Nashville, TN, ²Vanderbilt University Medical Center, Nashville, TN, ³Columbia University Medical Center, New York, NY
*Purpose: Although machine perfusion has gained momentum as an organ preservation strategy in liver transplantation, significant organ shortages and waitlist mortality persist, highlighting unmet needs…
2021 American Transplant Congress
Successful Long Term Survival of Vascularized Composite Allografts After Extended Preservation at Subzero Temperatures Using Bioinspired Next Generation Cryoprotectants
M. Kline¹, S. Fidder², A. Callegari¹, A. Matoso², B. Oh², K. Lombardo², J. Etra², D. Vasilic³, A. Childs¹, R. Redett², G. Brandacher², X. Wei¹
¹X-Therma Inc., Richmond, CA, ²Johns Hopkins University School of Medicine, Baltimore, MD, ³Erasmus MC, Rotterdam, Netherlands
*Purpose: Vascularized composite allotransplantation (VCA) is a viable reconstructive option for patients with devastating tissue defects. However, the field is constrained by short tolerable ischemia…
2021 American Transplant Congress
Patterns and Inhibition of Cell Death During Static Cold Storage of Human Kidneys
J. R. DiRito¹, G. Chickering², J. T. Langford¹, D. Stern¹, S. Hosgood³, M. L. Nicholson³, E. Gavathiotis⁴, R. N. Kitsis⁵, M. Saltzman², J. S. Pober⁶, G. T. Tietjen⁷
¹Surgery, Yale School of Medicine, New Haven, CT, ²Biomedical Engineering, Yale University, New Haven, CT, ³Surgery, University of Cambridge, Cambridge, United Kingdom, ⁴Biochemistry and Medicine, Wilf Family Cardiovascular Research Institute, Albert Einstein College of Medicine, Bronx, NY, ⁵Surgery, Albert Einstein College of Medicine, Bronx, NY, ⁶Immunobiology, Yale University, New Haven, CT, ⁷Surgery and Biomedical Engineering, Yale School of Medicine, New Haven, CT
*Purpose: As the donor population continues to age and accumulate co-morbidities, more marginal kidneys are being retrieved for transplantation. However, estimates suggest that more than…
2021 American Transplant Congress
The Harmful Effect of Retained Peripheral Cells During Flushing and Static Cold Storage in Rat Liver Normothermic Machine Perfusion
O. Haque¹, C. A. Pendexter², J. F. Markmann², K. Uygun², H. Yeh², S. N. Tessier²
¹Surgery, Beth Israel Deaconess Medical Center, Boston, MA, ²Massachusetts General Hospital, Boston, MA
*Purpose: Retained donor blood in a liver graft can cause damage to resident cells that is exacerbated by static cold storage (SCS), leading to platelet…
2021 American Transplant Congress
Combining Oxygenated Cold Perfusion with Normothermic Ex-vivo Perfusion Improves the Outcome of DCD Porcine Kidney Transplantation
L. I. Mazilescu¹, T. Goto¹, R. Rosales¹, Y. Noguchi¹, P. Urbanellis¹, L. A. Robinson², M. Selzner¹
¹Ajmera Transplant Program, Toronto General Hospital, Toronto, ON, Canada, ²Department of Nephrology, The Hospital for Sick Children, Toronto, ON, Canada
*Purpose: Ex-vivo machine perfusion is a novel preservation technique for the storage and assessment of marginal kidney grafts. Normothermic (NEVKP) as well as hypothermic machine…
2021 American Transplant Congress
Determining Endpoint Criteria in Ex Vivo Normothermic Limb Perfusion (EVNLP)
S. K. Pandey¹, A. Meyers¹, P. Sadeghi¹, V. Kopparthy¹, T. Xia¹, H. Brunengraber², S. Dasarathy³, A. Rampazzo¹, B. Bassiri Gharb¹
¹Plastic Surgery, Cleveland Clinic, Cleveland, OH, ²Department of Nutrition, School of Medicine, Case Western Reserve University, Cleveland, OH, ³Department of Gastroenterology, Cleveland Clinic, Cleveland, OH
*Purpose: There are no established criteria for discontinuing EVNLP before irreversible muscle and endothelial cell damage occur. This study aimed to evaluate weight gain as…
2021 American Transplant Congress
Efficacy of Ex Vivo Normothermic Limb Perfusion in Maintaining Cellular Viability and Muscle Function
T. Xia¹, P. Sadeghi², V. Kopparthy², S. Pandey², A. Rampazzo², B. Bassiri Gharb²
¹School of Medicine, Case Western Reserve University, Cleveland, OH, ²Department of Plastic Surgery, Cleveland Clinic, Cleveland, OH
*Purpose: The purpose of this study was to evaluate cellular injury and myocyte function of amputated limbs following ex vivo normothermic limb perfusion (EVNLP) compared…
2021 American Transplant Congress
The Edge of Glory: Determinants of Success and Failure for Prolonged Cold Ischemia in Kidney Transplantation
A. Turner¹, M. Hamidi¹, V. Ariyamuthu², R. Harland¹, B. Tanriover²
¹Surgery, Banner University of Arizona Medical Center, Tucson, AZ, ²Medicine, Banner University of Arizona Medical Center, Tucson, AZ
*Purpose: Prolonged cold ischemia time (CIT>30 hours) is associated with an increased risk of primary non-function and early graft loss in kidney transplantation, although this…
2021 American Transplant Congress
Ex Situ Heart Perfusion and Standard of Care Cold Storage Differentially Affect the Ischemic Secretome of Donor Hearts in Perfusates but Not the Reperfusion Response in Recipient Plasma
E. Chichelnitskiy¹, B. Wiegmann², N. Ledwoch¹, F. Ius², F. Wandrer¹, J. Kühne¹, K. Beushausen¹, J. Keil¹, S. V. Rojas², W. Sommer², C. Kühn², I. Tudorache², M. Avsar², A. Haverich², G. Warnecke², C. S. Falk¹
¹MHH, Institute of Transplant Immunology, Hannover, Germany, ²MHH, Department for Cardiothoracic, Transplantation and Vascular Surgery, Hannover, Germany
*Purpose: Various allograft preservation procedures influence the donor organ status that may be reflected by the cytokine/chemokine microenvironment in heart transplant (HTx) recipients. In addition,…