Articles tagged "Pig"

2020 American Transplant Congress
Coagulation and Cd46 Transgene Modification in Neonatal Porcine Islet Xenotransplantation
M. Song, Z. Fitch, K. Samy, Q. Gao, R. P. Davis, F. Leopard, R. Schmitz, N. Huffman, G. Devi, B. Collins, A. D. Kirk
Surgery, Duke University, Durham, NC
*Purpose: Vascular thrombosis is a potential cause for early intraportal xeno-islet loss. To better understand the relationship between coagulation and inflammation in early xeno-islet transplantation,…
2020 American Transplant Congress
What Happens When You Administer Mesenchymal Stromal Cells During Normothermic Machine Perfusion of Porcine Kidneys: Possible and Helpful or Not?
K. Rozenberg¹, L. Knijff¹, J. Sierra-Parraga², L. Lo Faro¹, M. Hoogduijn², C. Baan², J. Hunter¹, R. Ploeg¹
¹Nuffield Department of Surgical Sciences, University of Oxford, Oxford, United Kingdom, ²Department of Internal Medicine, Nephrology and Transplantation, Erasmus MC, University Medical Center, Rotterdam, Netherlands
*Purpose: Donation after circulatory death kidneys are widely used to increase the donor pool, despite their inferior quality. Normothermic machine perfusion (NMP) could provide a…
2020 American Transplant Congress
Normothermic Ex-Vivo Kidney Perfusion is Superior to Anoxic and Oxygenated Hypothermic Machine Perfusion for the Preservation of DCD Porcine Kidney Grafts
L. Mazilescu¹, M. Kaths², P. Urbanellis¹, M. Hamar³, T. Goto¹, I. Linares-Cervantes¹, Y. Noguchi¹, A. Konvalinka¹, A. Ghanekar¹, D. Bagli⁴, R. John⁵, L. Robinson⁶, M. Selzner¹
¹Multi Organ Transplant Program, Toronto General Hospital, Toronto, ON, Canada, ²Department of General, Visceral, and Transplantation Surgery, University Hospital Essen, Essen, Germany, ³Department of Transplantation and Surgery, Semmelweis University, Budapest, Hungary, ⁴Departments of Surgery(Urology)&Physiology, The Hospital for Sick Children, Toronto, ON, Canada, ⁵Laboratory Medicine & Pathobiology, Toronto General Hospital, Toronto, ON, Canada, ⁶Division of Nephrology, The Hospital for Sick Children, Toronto, ON, Canada
*Purpose: To increase the donor pool, better preservation strategies for the storage of marginal kidney grafts are crucial. Currently, kidney grafts are either stored on…
2020 American Transplant Congress
Improved Survival of Acute Liver Failure by “Off-the-Shelf” Bioartificial Liver Support System Using Immobilized Cryopreserved Hepatocyte Spheroids in Porcine Model
S. Lee¹, J. Lee², H. Park², Y. Kim², J. Noh³, J. Jung³, S. Lee³, H. Shin³, H. Yoon⁴, D. Lee³, S. Lee¹
¹Department of Surgery, Samsung Medical Center, Seoul, Korea, Republic of, ²Stem Cell and Regenerative Medicine Center, Research Institute for Future Medicine, Samsung Medical Center, Seoul, Korea, Republic of, ³Biomedical Research Center, Lifeliver, Co. Ltd., Yongin, Korea, Republic of, ⁴Biomedical Research Center, Lifeliver, Co. Ltd., Seoul, Korea, Republic of
*Purpose: Acute liver failure (ALF) has a rapid and unpredictable onset with high mortality. The only current treatment is to transplant a donor organ but…
2020 American Transplant Congress
Prolonged In Vivo Perfusion of a Re-Endothelialized Human-Scale Tissue Engineered Kidney Graft
J. S. Uzarski¹, E. E. Russell¹, E. C. Beck¹, M. Holzner², V. Wadhera², D. Adamson², T. Gilbert¹, S. Florman², D. S. Davidow¹, R. Shapiro², J. Ross¹
¹Research & Development, Miromatrix Medical Inc., Eden Prairie, MN, ²Recanati/Miller Institute, Mount Sinai, New York, NY
*Purpose: Advances are desperately needed to increase the supply of transplantable kidneys for the 100,000 patients on the waiting list. Whole organ engineering is one…
2020 American Transplant Congress
Xenoantibody Depletion of Human Blood with Normothermic Machine Perfusion of Porcine Kidney and Lung Prior to Liver Perfusion
T. M. Coe¹, N. Serifis¹, S. Raigani¹, C. Carroll¹, C. G. Rickert¹, R. Matheson¹, D. Detelich², W. Qin³, Y. Kan³, J. Layer³, M. Youd³, W. Westlin³, L. Yang³, A. Azimzadeh¹, J. F. Markmann¹
¹Surgery, Massachusetts General Hospital, Boston, MA, ²Surgery, Tufts Medical Center, Boston, MA, ³eGenesis, Cambridge, MA
*Purpose: Genetic modifications of porcine donors for pig-to-human xenotransplantation have led to significant progress in survival of xenografts in preclinical models. Triple knock-out (TKO) of…
2020 American Transplant Congress
Old World Monkeys Are Not Ideal Transplantation Models for Testing CMAHKO (TKO or GTKO/CMAHKO) Pig Kidneys
T. Yamamoto¹, H. Iwase¹, D. Patel¹, A. Jagdale¹, J. Foote¹, D. Ayares², D. Anderson¹, D. E. Eckhoff¹, D. K. Cooper¹, H. Hara¹
¹University of Alabama at Birmingham, Birmingham, AL, ²Revivicor, Blacksburg, VA
*Purpose: Triple-knockout (TKO) pigs are likely to be an optimal source of organs for transplantation into human recipients, many of whom do not have natural…
2020 American Transplant Congress
Enumeration of Platelets by Flow Cytometry Reveals Human Erythrocyte Fragmentation During Pig Organ Xenoperfusion Studies
Z. Abady¹, K. M. Petitpas¹, L. Burdorf¹, S. G. Pratts¹, B. M. Cerel¹, S. Sendil², M. R. Connolly¹, W. Qin³, Y. Kan³, J. Layer³, M. Youd³, W. Westlin³, L. Yang³, R. N. Pierson III¹, A. M. Azimzadeh¹
¹Surgery, Massachusetts General Hospital and Harvard School of Medicine, Boston, MA, ²Surgery, University of Maryland School of Medicine, Baltimore, MD, ³eGenesis, Cambridge, MA
*Purpose: Human or baboon platelet sequestration and activation occurs during pig organ xenograft perfusion, although the mechanism remains poorly understood. To evaluate an apparent “rebound”…
2020 American Transplant Congress
Immuno-Modulation of Primed Xenogeneic T Cell Response by mTOR Blockade of T Cells and Porcine Endothelial Cells
S. Li, A. Kirk, H. Xu
Surgery, Duke University, Durham, NC
*Purpose: Porcine vascular endothelial cells (PECs) play a central role in xeno-recognition and costimulation. The objectives of this study were to characterize the immuno-phenotype of…
2020 American Transplant Congress
Expression of Multiple Human Complement Regulatory Proteins Results in Prolonged Heterotopic Xenoheart Survival in an NHP Model
K. Ahrens¹, J. M. O¹, W. Sommer², D. Becerra¹, P. M. Patel¹, A. Dehnadi¹, Y. Kan³, J. Layer³, J. Morrissette¹, T. Costa¹, I. M. Hanekamp¹, J. S. Allan⁴, M. Youd³, R. N. Pierson⁵, W. Qin³, L. Burdorf⁵, A. M. Azimzadeh¹, W. Westlin³, L. Yang³, J. C. Madsen⁵
¹Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, ²Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany, ³eGenesis Inc., Cambridge, MA, ⁴Center for Transplantation Sciences, Division of Thoracic Surgery, Massachusetts General Hospital, Boston, MA, ⁵Center for Transplantation Sciences, Division of Cardiac Surgery, Massachusetts General Hospital, Boston, MA
*Purpose: Advances in genome engineering technology allow for multiplex genetic modifications in pigs designed to address immunologic and coagulation incompatibilities in order to facilitate xenotransplantation.…