Articles tagged "Pig"

2020 American Transplant Congress
Immuno-Modulation of Primed Xenogeneic T Cell Response by mTOR Blockade of T Cells and Porcine Endothelial Cells
S. Li, A. Kirk, H. Xu
Surgery, Duke University, Durham, NC
*Purpose: Porcine vascular endothelial cells (PECs) play a central role in xeno-recognition and costimulation. The objectives of this study were to characterize the immuno-phenotype of…
2020 American Transplant Congress
Expression of Multiple Human Complement Regulatory Proteins Results in Prolonged Heterotopic Xenoheart Survival in an NHP Model
K. Ahrens¹, J. M. O¹, W. Sommer², D. Becerra¹, P. M. Patel¹, A. Dehnadi¹, Y. Kan³, J. Layer³, J. Morrissette¹, T. Costa¹, I. M. Hanekamp¹, J. S. Allan⁴, M. Youd³, R. N. Pierson⁵, W. Qin³, L. Burdorf⁵, A. M. Azimzadeh¹, W. Westlin³, L. Yang³, J. C. Madsen⁵
¹Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, ²Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany, ³eGenesis Inc., Cambridge, MA, ⁴Center for Transplantation Sciences, Division of Thoracic Surgery, Massachusetts General Hospital, Boston, MA, ⁵Center for Transplantation Sciences, Division of Cardiac Surgery, Massachusetts General Hospital, Boston, MA
*Purpose: Advances in genome engineering technology allow for multiplex genetic modifications in pigs designed to address immunologic and coagulation incompatibilities in order to facilitate xenotransplantation.…
2020 American Transplant Congress
Sialoadhesin Mediates the Adhesion of Human RBC to Porcine Macrophages from Genetically Engineered Pigs
K. Petitpas¹, B. Cerel¹, Z. Abady¹, L. Burdorf¹, D. L. Ayares², D. M. Magnani³, R. N. Pierson III¹, A. M. Azimzadeh¹
¹Center for Transplantation Sciences, Massachusetts General Hospital/Harvard Medical School, Boston, MA, ²Revivicor, Blacksburg, VA, ³MassBiologics of UMMS, Boston, MA
*Purpose: The sequestration of human RBC is prominent after liver and lung xenotransplantation. Rees et al previously implicated porcine sialoadhesin in adhesion of human RBC…
2020 American Transplant Congress
The Development of an Innovative Training Pathway for Robotic Assisted Renal Transplant Program
H. Pournik¹, J. Castillo², V. Brown², J. Collins², C. Haisch², D. Leeser²
¹ECU, Greenville, NC, ²Surgery, East Carolina University, Greenville, NC
*Purpose: To develop and implement a surgeon and team training program for robotic renal transplants utilizing innovative technology and techniques.*Methods: A graduated robotic educational program…
2020 American Transplant Congress
Using an Ex Vivo Blood-Free Model of Preservation and Reperfusion to Evaluate the Effects of Subnormothermic Perfusion with Hydrogen Sulfide on DCD Porcine Renal Grafts
S. Juriasingani¹, A. Ruthirakanthan¹, M. Richard-Mohamed², M. Zhang¹, M. Levine¹, E. Sogutdelen¹, M. Mandurah¹, A. Sener¹
¹University of Western Ontario, London, ON, Canada, ²London Health Sciences Centre, London, ON, Canada
*Purpose: The need for donor blood complicates the clinical translation of normothermic and subnormothermic perfusion for preservation and graft function evaluation. As such, hemoglobin-based oxygen…
2020 American Transplant Congress
A Large Animal Model of Ex-Vivo Lung Perfusion as a Platform to Deliver Therapeutic Agents to Lungs Before Lung Transplant
T. M. Egan¹, B. Haithcock¹, M. H. Ali¹, J. W. Blackwell¹, B. Masuodi¹, S. Shipley², T. Long³, A. Akerman¹, S. Montgomery⁴, H. Yuan⁵, P. Stewart⁶, J. Potenziano⁷, C. Sekaran⁷, R. Fitch⁷
¹Surgery, UNC at Chapel Hill, Chapel Hill, NC, ²Department of Pathology and Laboratory Medicine, UNC at Chapel Hill, Chapel Hill, NC, ³Pathology and Laboratory Medicine,, UNC at Chapel Hill, Chapel Hill, NC, ⁴Pathology and Laboratory Medicine, UNC at Chapel Hill, Chapel Hill, NC, ⁵Radiology, UNC at Chapel Hill, Chapel Hill, NC, ⁶Gillings Global School of Public Health, UNC at Chapel Hill, Chapel Hill, NC, ⁷Mallinckrodt Pharmaceuticals, Hazelwood, MO
*Purpose: Ex-vivo lung perfusion (EVLP) has been used to evaluate lung function before lung transplant (LT). We used EVLP to expose lungs recovered 6 hours…
2020 American Transplant Congress
Experimental Porcine Pancreatic Allotransplantation after Hypothermic Perfusion
T. Prudhomme¹, D. Kervella¹, K. Renaudin², V. Gauttier¹, S. Le Bas-Bernardet³, D. Minault³, J. Hervouet³, D. Cantarovich¹, G. Karam¹, G. Blancho¹, J. Branchereau¹
¹Institut de Transplantation Urologie Néphrologie (ITUN), CHU Nantes, Nantes, France, ²Département d’Anatomie et de Cytologie Pathologique, CHU Nantes, Nantes, France, ³Centre de Recherche en Transplantation et Immunologie, UMR1064, INSERM, Université de Nantes, Nantes, France
*Purpose: The main objective of this study was to compare the different preservation techniques (static cold storage (SCS) and hypothermic pulsatile perfusion (HPP)) in a…
2020 American Transplant Congress
XIAP Enhances Xenoislet Survival: Novel Models to Evaluate Pretransplantation Gene Modifications
G. R. Devi¹, Z. Fitch¹, M. Song¹, M. Ribeiro¹, A. McGowan¹, X. Luo², B. H. Collins¹, A. D. Kirk¹
¹Department of Surgery, Duke Univesity School of Medicine, Durham, NC, ²Division of Nephrology, Duke Univesity School of Medicine, Durham, NC
*Purpose: Porcine islet xenotransplantation offers a reliable and near limitless supply of islets for the treatment of type 1 diabetes. Intraportal infusion of cells exposes…
2020 American Transplant Congress
Hepatic Microenvironment: Molecular and Chemical Changes Associated with Preconditioning Maneuvers
I. Lies, C. Watkins, M. Em, J. D. Eason, C. Eynard, T. Rousselle, V. R. Mas, D. Maluf
Dept of Surgery, Transplant Research Institute, James D. Eason Transplant Institute, University of Tennessee Health Science Center, Memphis, TN
*Purpose: Liver preconditioning has been described to protect liver from ischemia injury and trigger liver regeneration. Hereby, we aimed to assess the effect of preconditioning…
2020 American Transplant Congress
Abdominal Multiorgan Retrieval from Pigs in a Slaughterhouse: A Reliable and Efficient Donation after Circulatory Death Model for Ex Vivo Organ Perfusion
F. Neri, F. Dengu, G. Ebeling, L. Knijff, K. Rozemberg, J. Branchereau, P. Friend, R. Ploeg, J. Hunter
Nuffield Department of Surgical Sciences, Oxford University, Oxford, United Kingdom
*Purpose: Advances in organ perfusion technology have been driven by the increasing necessity to utilise organs from donors after circulatory death (DCD) and marginal donors.…