Articles tagged "Pig"

2020 American Transplant Congress
Prolonged In Vivo Perfusion of a Re-Endothelialized Human-Scale Tissue Engineered Kidney Graft
J. S. Uzarski¹, E. E. Russell¹, E. C. Beck¹, M. Holzner², V. Wadhera², D. Adamson², T. Gilbert¹, S. Florman², D. S. Davidow¹, R. Shapiro², J. Ross¹
¹Research & Development, Miromatrix Medical Inc., Eden Prairie, MN, ²Recanati/Miller Institute, Mount Sinai, New York, NY
*Purpose: Advances are desperately needed to increase the supply of transplantable kidneys for the 100,000 patients on the waiting list. Whole organ engineering is one…
2020 American Transplant Congress
Xenoantibody Depletion of Human Blood with Normothermic Machine Perfusion of Porcine Kidney and Lung Prior to Liver Perfusion
T. M. Coe¹, N. Serifis¹, S. Raigani¹, C. Carroll¹, C. G. Rickert¹, R. Matheson¹, D. Detelich², W. Qin³, Y. Kan³, J. Layer³, M. Youd³, W. Westlin³, L. Yang³, A. Azimzadeh¹, J. F. Markmann¹
¹Surgery, Massachusetts General Hospital, Boston, MA, ²Surgery, Tufts Medical Center, Boston, MA, ³eGenesis, Cambridge, MA
*Purpose: Genetic modifications of porcine donors for pig-to-human xenotransplantation have led to significant progress in survival of xenografts in preclinical models. Triple knock-out (TKO) of…
2020 American Transplant Congress
Old World Monkeys Are Not Ideal Transplantation Models for Testing CMAHKO (TKO or GTKO/CMAHKO) Pig Kidneys
T. Yamamoto¹, H. Iwase¹, D. Patel¹, A. Jagdale¹, J. Foote¹, D. Ayares², D. Anderson¹, D. E. Eckhoff¹, D. K. Cooper¹, H. Hara¹
¹University of Alabama at Birmingham, Birmingham, AL, ²Revivicor, Blacksburg, VA
*Purpose: Triple-knockout (TKO) pigs are likely to be an optimal source of organs for transplantation into human recipients, many of whom do not have natural…
2020 American Transplant Congress
Enumeration of Platelets by Flow Cytometry Reveals Human Erythrocyte Fragmentation During Pig Organ Xenoperfusion Studies
Z. Abady¹, K. M. Petitpas¹, L. Burdorf¹, S. G. Pratts¹, B. M. Cerel¹, S. Sendil², M. R. Connolly¹, W. Qin³, Y. Kan³, J. Layer³, M. Youd³, W. Westlin³, L. Yang³, R. N. Pierson III¹, A. M. Azimzadeh¹
¹Surgery, Massachusetts General Hospital and Harvard School of Medicine, Boston, MA, ²Surgery, University of Maryland School of Medicine, Baltimore, MD, ³eGenesis, Cambridge, MA
*Purpose: Human or baboon platelet sequestration and activation occurs during pig organ xenograft perfusion, although the mechanism remains poorly understood. To evaluate an apparent “rebound”…
2020 American Transplant Congress
Immuno-Modulation of Primed Xenogeneic T Cell Response by mTOR Blockade of T Cells and Porcine Endothelial Cells
S. Li, A. Kirk, H. Xu
Surgery, Duke University, Durham, NC
*Purpose: Porcine vascular endothelial cells (PECs) play a central role in xeno-recognition and costimulation. The objectives of this study were to characterize the immuno-phenotype of…
2020 American Transplant Congress
Expression of Multiple Human Complement Regulatory Proteins Results in Prolonged Heterotopic Xenoheart Survival in an NHP Model
K. Ahrens¹, J. M. O¹, W. Sommer², D. Becerra¹, P. M. Patel¹, A. Dehnadi¹, Y. Kan³, J. Layer³, J. Morrissette¹, T. Costa¹, I. M. Hanekamp¹, J. S. Allan⁴, M. Youd³, R. N. Pierson⁵, W. Qin³, L. Burdorf⁵, A. M. Azimzadeh¹, W. Westlin³, L. Yang³, J. C. Madsen⁵
¹Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, ²Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany, ³eGenesis Inc., Cambridge, MA, ⁴Center for Transplantation Sciences, Division of Thoracic Surgery, Massachusetts General Hospital, Boston, MA, ⁵Center for Transplantation Sciences, Division of Cardiac Surgery, Massachusetts General Hospital, Boston, MA
*Purpose: Advances in genome engineering technology allow for multiplex genetic modifications in pigs designed to address immunologic and coagulation incompatibilities in order to facilitate xenotransplantation.…
2020 American Transplant Congress
Sialoadhesin Mediates the Adhesion of Human RBC to Porcine Macrophages from Genetically Engineered Pigs
K. Petitpas¹, B. Cerel¹, Z. Abady¹, L. Burdorf¹, D. L. Ayares², D. M. Magnani³, R. N. Pierson III¹, A. M. Azimzadeh¹
¹Center for Transplantation Sciences, Massachusetts General Hospital/Harvard Medical School, Boston, MA, ²Revivicor, Blacksburg, VA, ³MassBiologics of UMMS, Boston, MA
*Purpose: The sequestration of human RBC is prominent after liver and lung xenotransplantation. Rees et al previously implicated porcine sialoadhesin in adhesion of human RBC…
2020 American Transplant Congress
The Development of an Innovative Training Pathway for Robotic Assisted Renal Transplant Program
H. Pournik¹, J. Castillo², V. Brown², J. Collins², C. Haisch², D. Leeser²
¹ECU, Greenville, NC, ²Surgery, East Carolina University, Greenville, NC
*Purpose: To develop and implement a surgeon and team training program for robotic renal transplants utilizing innovative technology and techniques.*Methods: A graduated robotic educational program…
2020 American Transplant Congress
Using an Ex Vivo Blood-Free Model of Preservation and Reperfusion to Evaluate the Effects of Subnormothermic Perfusion with Hydrogen Sulfide on DCD Porcine Renal Grafts
S. Juriasingani¹, A. Ruthirakanthan¹, M. Richard-Mohamed², M. Zhang¹, M. Levine¹, E. Sogutdelen¹, M. Mandurah¹, A. Sener¹
¹University of Western Ontario, London, ON, Canada, ²London Health Sciences Centre, London, ON, Canada
*Purpose: The need for donor blood complicates the clinical translation of normothermic and subnormothermic perfusion for preservation and graft function evaluation. As such, hemoglobin-based oxygen…
2020 American Transplant Congress
A Large Animal Model of Ex-Vivo Lung Perfusion as a Platform to Deliver Therapeutic Agents to Lungs Before Lung Transplant
T. M. Egan¹, B. Haithcock¹, M. H. Ali¹, J. W. Blackwell¹, B. Masuodi¹, S. Shipley², T. Long³, A. Akerman¹, S. Montgomery⁴, H. Yuan⁵, P. Stewart⁶, J. Potenziano⁷, C. Sekaran⁷, R. Fitch⁷
¹Surgery, UNC at Chapel Hill, Chapel Hill, NC, ²Department of Pathology and Laboratory Medicine, UNC at Chapel Hill, Chapel Hill, NC, ³Pathology and Laboratory Medicine,, UNC at Chapel Hill, Chapel Hill, NC, ⁴Pathology and Laboratory Medicine, UNC at Chapel Hill, Chapel Hill, NC, ⁵Radiology, UNC at Chapel Hill, Chapel Hill, NC, ⁶Gillings Global School of Public Health, UNC at Chapel Hill, Chapel Hill, NC, ⁷Mallinckrodt Pharmaceuticals, Hazelwood, MO
*Purpose: Ex-vivo lung perfusion (EVLP) has been used to evaluate lung function before lung transplant (LT). We used EVLP to expose lungs recovered 6 hours…