Articles tagged "Pig"

2022 American Transplant Congress
Implementation of Infectious Disease Surveillance in Recently Deceased Human Xenograft Study
S. J. Cawthon, E. P. Weldon, N. S. Lawson, B. E. Lonze, R. A. Montgomery, S. A. Mehta
NYU Langone Transplant Institute, New York, NY
*Purpose: Xenotransplantation is a promising solution for the ongoing organ shortage but has been slow to develop because of both immunological challenges and potential risk…
2022 American Transplant Congress
Manufactured Organ Procurement Facility Collaboration and Authorization Approach for Xenotransplant Research
E. P. Weldon, N. S. Lawson, B. E. Sullivan, B. S. Parent, C. L. Deterville, R. A. Dieter, R. A. Montgomery
NYU Langone Health-Transplant Institute, New York, NY
*Purpose: A Manufactured Organ Research Facility (MORF) was created at our institution to provide a safe environment for testing xenograft organs in deceased brain dead…
2022 American Transplant Congress
Enhancing Early Nerve Regeneration from Reconstruction of a Segmental Nerve Injury with Polyethylene Glycol
A. Bernal¹, C. Hinshaw¹, J. Hinshaw¹, C. Yang², S. Margaux¹, W. Zhang¹, J. Nuelle¹, C. Sabbag¹, G. Bittner², J. Shores³, E. Weitzel¹, J. Alderete¹
¹RESTOR FUSE Nerve Reconstruction Consortium, US Army Institute of Surgical Research/59th MDW, San Antonio, TX, ²University of Texas at Austin, Austin, TX, ³John Hopkins University, Baltimore, MD
*Purpose: Despite advancements in understanding and treating peripheral nerve injuries (PNIs), restoration of lost motor and sensory functions remain poor using current methods of clinical…
2022 American Transplant Congress
Long-Term (>1 Year) Rejection Free Graft Survival of Kidney Xenografts with Triple Xenoantigen Knockout and Multiple Human Transgenes in Cynomolgus Monkeys
T. Hirose¹, G. Lassiter¹, D. Ma¹, A. D'Attilio¹, I. Rosales¹, D. Cloonan¹, R. Matheson¹, R. Colvin¹, W. Qin², Y. Kan², J. Layer², R. Anand², V. Paragas², L. Queroz², X. Tan², I. Kohnle², K. Stiede², K. Hall², M. Youd², M. Curtis², J. Markmann¹, T. Kawai¹
¹Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, ²eGenesis Inc, Cambridge, MA
*Purpose: Pigs with deletion of 3 carbohydrate xenoantigens (triple knock-out, TKO) are expected to be optimal donors for human xenotransplantation. We have previously shown that…
2021 American Transplant Congress
Combined CD11b/CD40 Blockade is Superior to CD40 Blockade Alone in Prolonging Survival in Pig-to-Nonhuman Primate Renal Xenotransplantation
D. A. Faber¹, B. Lovasik¹, A. Matar¹, C. Breeden¹, S. Kim¹, A. Adams²
¹Surgery, Emory University School of Medicine, Atlanta, GA, ²Surgery, University of Minnesota, Minneapolis, MN
*Purpose: Blockade of the CD40/CD154 pathway is highly effective in preventing rejection in pig-to-nonhuman primate (NHP) xenotransplantation models. Clinical translation of anti-CD154 mAbs, however, has…
2021 American Transplant Congress
Complement Blockade Improves Renal Xenograft Survival in Primates in the Absence of Transgenic Complement Regulatory Proteins
D. A. Faber¹, B. Lovasik¹, A. Matar¹, C. Breeden¹, A. B. Farris², M. Tector³, A. Tector³, A. Adams⁴
¹Surgery, Emory University School of Medicine, Atlanta, GA, ²Pathology, Emory University School of Medicine, Atlanta, GA, ³Surgery, University of Miami, Miami, FL, ⁴Surgery, University of Minnesota, Minneapolis, MN
*Purpose: Complement activation is a major contributor to xenograft rejection. To minimize xenograft injury, complement suppression via insertion of human complement-regulatory transgenes into the donor…
2021 American Transplant Congress
Cold Perfusion for Ischemia Minimization Reduces Cardiac Injury in an Ex Vivo Xenoperfusion Model
M. R. Connolly¹, A. Calhoun¹, F. Pollok¹, L. Burdorf¹, Z. Habibabady¹, M. Ma¹, S. Miura¹, W. Eyestone², C. Phelps², D. Ayares²
¹Massachusetts General Hospital, Boston, MA, ²Revivicor, Blacksburg, VA
*Purpose: Prior studies using Steen’s cold perfusion storage technique for heart ischemia minimization (IM) prevented initial xenograft dysfunction (IXD). Our pilot data evaluates the effect…
2021 American Transplant Congress
Imaging Flow Cytometry Reveals That Platelet-Sized Erythrocyte Fragments are Formed During Pig Organ Perfusion with Human Blood
Z. A. Habibabady¹, F. Ellett², L. Burdorf¹, M. Connolly¹, F. Pollok¹, D. Irimia², R. N. Pierson III¹, A. M. Azimzadeh¹
¹Center for Transplantation Sciences, Massachusetts General Hospital and Harvard School of Medicine, Boston, MA, ²Center for Engineering in Medicine and Surgery, Massachusetts General Hospital and Harvard School of Medicine, Boston, MA
*Purpose: Severe thrombocytopenia frequently occurs after xenotransplantation of porcine cells or organs, and when pig organs are perfused with human blood. We developed a flow…
2021 American Transplant Congress
Initial Experience with Growth Hormone Receptor Knockout in Pig-to-Baboon Kidney Transplantation: Effect on Ureteric Viability and Kidney Growth
C. Hansen-Estruch¹, H. Iwase¹, A. Jagdale¹, T. Yamamoto¹, M. H. Bikhet¹, J. Foote², H. Hara¹, D. Anderson¹, P. Porrett¹, D. Eckhoff¹, D. Ayares³, W. Eyestone³, J. Locke¹, D. K. Cooper¹
¹Xenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, Birmingham, AL, ²Department of Microbiology and Animal Resources Program, University of Alabama at Birmingham, Birmingham, AL, ³Revivicor, Blacksburg, VA
*Purpose: The purpose of this study was to report outcomes of pig-to-baboon renal transplants using a novel growth hormone receptor knockout (GHRKO) organ-source pig created…
2021 American Transplant Congress
Anti-pig IgE and IgA Antibodies in Sera of (i) Naïve Primates and (ii) Non-human Primates Sensitized to Pig Xenografts
H. Hara, Q. Li, H. Iwase, T. Yamamoto, D. Ayares, D. K. Cooper
University of Alabama at Birmingham, Birmingham, AL
*Purpose: Anti-pig IgM and IgG antibodies in primates play an important role in xenograft rejection. The possible roles of anti-pig IgE and IgA have not…