Articles tagged "Pig"

2022 American Transplant Congress
Fibrin Deposition in Ischemic Damaged Liver Grafts and Reconditioning with Thrombolytic Therapy During Normothermic Machine Perfusion
T. Goto, Y. Noguchi, S. Ganesh, B. Arulratnam, M. Kawamura, C. Parmentier, S. Ray, T. Reichman, N. Selzner, M. Selzner
Ajmera Transplant Program, Department of Surgery, Toronto General Hospital, University Health Network, Toronto, ON, Canada
*Purpose: Normothermic ex situ perfusion (NMP) is a novel technique for the storage and assessment of marginal livers retrieved from donors after circulatory death (DCD).…
2022 American Transplant Congress
Perfusate Cell-Free DNA Content is a Potential Marker of Cellular Injury During Hypothermic Machine Perfusion of Porcine Kidneys Subject to Prolonged Warm Ischemia
V. Boominathan¹, M. Willman¹, N. Battula², A. Zarrinpar¹, S. Duarte¹
¹University of Florida, Gainesville, FL, ²University of Oklahoma, Oklahoma City, OK
*Purpose: Despite the potential benefits and expanding use of advanced dynamic perfusion systems in clinical practice, to date there is no robust and non-invasive method…
2022 American Transplant Congress
Utility of Porcine Kidneys in Transplantation Model for Training General Surgery Residents
O. Eletta, V. Putatunda, D. Walls, C. Antwi-Buosiako, B. Perry, N. Maloney Patel, R. Pelletier, A. Bongu
Rutgers Robert Wood Johnson Medical School, New Brunswick, NJ
*Purpose: We sought to increase the opportunities for general surgery residents to hone their vascular anastomosis skills. Accordingly, we created a kidney transplant model using…
2022 American Transplant Congress
Local Mesenchymal Stem Cell Therapy for the Ex Vivo Preservation of a Porcine Vascularized Composite Allograft with 24-hour Normothermic Machine Perfusion
A. Bernal¹, J. Hinshaw¹, J. Pena¹, C. Hinshaw¹, D. Wood¹, K. Zimmern², N. McMahon², J. Ling², W. Zhang¹, E. Weitzel¹
¹US Army Institute of Surgical Research/59th MDW, San Antonio, TX, ²Southwest Research Institute, San Antonio, TX
*Purpose: Given therapeutic limitations in limb salvage, we sought to demonstrate the efficacy of combining local mesenchymal stem cells (MSCs) infusion with fresh whole blood…
2021 American Transplant Congress
Cold Perfusion for Ischemia Minimization Reduces Cardiac Injury in an Ex Vivo Xenoperfusion Model
M. R. Connolly¹, A. Calhoun¹, F. Pollok¹, L. Burdorf¹, Z. Habibabady¹, M. Ma¹, S. Miura¹, W. Eyestone², C. Phelps², D. Ayares²
¹Massachusetts General Hospital, Boston, MA, ²Revivicor, Blacksburg, VA
*Purpose: Prior studies using Steen’s cold perfusion storage technique for heart ischemia minimization (IM) prevented initial xenograft dysfunction (IXD). Our pilot data evaluates the effect…
2021 American Transplant Congress
Imaging Flow Cytometry Reveals That Platelet-Sized Erythrocyte Fragments are Formed During Pig Organ Perfusion with Human Blood
Z. A. Habibabady¹, F. Ellett², L. Burdorf¹, M. Connolly¹, F. Pollok¹, D. Irimia², R. N. Pierson III¹, A. M. Azimzadeh¹
¹Center for Transplantation Sciences, Massachusetts General Hospital and Harvard School of Medicine, Boston, MA, ²Center for Engineering in Medicine and Surgery, Massachusetts General Hospital and Harvard School of Medicine, Boston, MA
*Purpose: Severe thrombocytopenia frequently occurs after xenotransplantation of porcine cells or organs, and when pig organs are perfused with human blood. We developed a flow…
2021 American Transplant Congress
Initial Experience with Growth Hormone Receptor Knockout in Pig-to-Baboon Kidney Transplantation: Effect on Ureteric Viability and Kidney Growth
C. Hansen-Estruch¹, H. Iwase¹, A. Jagdale¹, T. Yamamoto¹, M. H. Bikhet¹, J. Foote², H. Hara¹, D. Anderson¹, P. Porrett¹, D. Eckhoff¹, D. Ayares³, W. Eyestone³, J. Locke¹, D. K. Cooper¹
¹Xenotransplantation Program, Department of Surgery, University of Alabama at Birmingham, Birmingham, AL, ²Department of Microbiology and Animal Resources Program, University of Alabama at Birmingham, Birmingham, AL, ³Revivicor, Blacksburg, VA
*Purpose: The purpose of this study was to report outcomes of pig-to-baboon renal transplants using a novel growth hormone receptor knockout (GHRKO) organ-source pig created…
2021 American Transplant Congress
Anti-pig IgE and IgA Antibodies in Sera of (i) Naïve Primates and (ii) Non-human Primates Sensitized to Pig Xenografts
H. Hara, Q. Li, H. Iwase, T. Yamamoto, D. Ayares, D. K. Cooper
University of Alabama at Birmingham, Birmingham, AL
*Purpose: Anti-pig IgM and IgG antibodies in primates play an important role in xenograft rejection. The possible roles of anti-pig IgE and IgA have not…
2021 American Transplant Congress
Triple-Knockout Pig Red Blood Cells Are a Potential Alternative Source for Clinical Blood Transfusion
T. Yamamoto¹, M. H. Bikhet¹, H. Q. Nguyen¹, M. Javed¹, D. Ayares², H. Iwase¹, H. Hara¹, D. K. Cooper¹
¹Xenotransplantation Program, Surgery, The University of Alabama at Birmingham, Birmingham, AL, ²Revivicor, Blacksburg, VA
*Purpose: Triple-knockout (TKO) pig RBCs (pRBCs) may be an alternative source for clinical blood transfusion because almost humans have no preformed antibody to TKO pRBCs.…
2021 American Transplant Congress
Composition of Ex Vivo Lung Perfusion Solutions and Kinetics Define Differential Cytokine and Chemokine Secretion in a Porcine Cardiac Arrest Model of Lung Preservation
L. M. Radomsky¹, A. Koch², C. Olbertz², J. F. Kuehne¹, Y. Liu², J. Keil¹, K. Beushausen¹, U. Rauen³, C. S. Falk¹, M. Kamler²
¹Institute of Transplant Immunology, Hannover Medical School, Hannover, Germany, ²Thoracic Transplantation, Department of Thoracic and Cardiovascular Surgery, University Hospital Essen, Essen, Germany, ³Institute of Physiological Chemistry, University Hospital Essen, Essen, Germany
*Purpose: Ex vivo lung perfusion is an innovative technique to evaluate marginal lung organs especially after DCD. Normothermic continuous perfusion should reduce ischemic damage and…