Articles tagged "Mixed chimerism"

2016 American Transplant Congress
Contrasting Effects of Tacrolimus and Everolimus on Regulatory T Cell Activation and Mixed Hematopoietic Chimerism Induced by Natural Killer T Cell Stimulation.
S. Miyairi,^1,2 T. Hirai,¹ R. IShii,¹ M. Okumi,¹ Y. Ishii,³ S. Nunoda,² K. Yamazaki,² K. Tanabe.¹
¹Urology, Tokyo Women's Medical University, Tokyo, Japan; ²Cardiovascular Surgery, Tokyo Women's Medical University, Tokyo, Japan; ³Laboratory of Vaccine Design, RIKEN IMS-RCAI, Yokohama, Japan.
Calcineurin (CN)-dependent IL-2 production is crucial for regulatory T cells (Tregs) to demonstrate suppressive activity. The mTOR signal is not required for Treg function because…
2016 American Transplant Congress
Delaying the Induction of Mixed Chimerism Until Two Months After NHP Heart and Kidney Cotransplantation Results in Long Term Survival.
K. Huh,¹ M. Tonsho,¹ P. Spencer,¹ J. Paster,¹ X. Wu,¹ I. Hanekamp,¹ T. Kawai,^1,3 R. Smith,² A. Cosimi,¹ A. Dehnadi,¹ G. Benichou,¹ R. Colvin,^1,2 J. Madsen.^1,4
¹Center for Transplantation Sciences, Department of Surgery, Massachusetts General Hospital, Boston, MA; ²Department of Pathology, Massachusetts General Hospital, Boston, MA; ³Department of Surgery, Massachusetts General Hospital, Boston, MA; ⁴Division of Cardiac Surgery, Department of Surgery, Massachusetts General Hospital, Boston, MA.
Background. We have previously achieved delayed tolerance in cynomologous recipients cotransplanted with heart and kidney allografts by treating recipients with standard triple drug immunosuppression for…
2016 American Transplant Congress
Immune Tolerance Monitoring in Renal Allograft Tolerance Induced by Transient Mixed Chimerism in Nonhuman Primates and Humans.
K. Hotta, T. Oura, K. Crisalli, D. Sachs, B. Cosim, T. Kawai.
Center for Transplantation Science, Massachusetts General Hospital, Boston, MA.
Background: Successful induction of allograft tolerance in MHC-mismatched kidney transplantation has been achieved after induction of transient chimerism in both nonhuman primates (NHP) and humans.…
2016 American Transplant Congress
IL-10 Expression of In Vitro Activated B Cells as A Biomarker for B Cell Tolerance.
T. Oura, K. Hotta, K. Crisalli, B. Cosimi, T. Kawai.
Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA.
Background: A significant role for regulatory B cell has been suggested in kidney transplant recipients who achieved operational tolerance while receiving conventional immunosuppression. We studied…
2015 American Transplant Congress
Rejection of an Allograft Through Non-MHC Antigens Does Not Translate to the Rejection of a Different Allograft from the Same Donor in Mixed Chimeras
K. Shinoda,¹ C. Chase,¹ P. Russell,¹ J. Madsen,¹ R. Colvin,² A. Alessandrini.¹
¹Transplant Center, Massachusetts General Hospital, Boston, MA; ²Pathology, Massachusetts General Hospital, Boston, MA.
We previously showed that tolerance, induced by mixed chimerism in mice, is antigen-specific: tolerance of heart and skin allografts is dependent on Foxp3+ cells that…
2015 American Transplant Congress
Regulatory T Cells Expanded by Activated iNKT Cells Facilitate Migration of Donor Dendritic Cells Into the Recipient Thymus and Subsequent Establishment of Clonal Deletion
T. Hirai,¹ R. Shibahara,¹ S. Miyairi,¹ M. Ikemiyagi,¹ K. Omoto,¹ Y. Ishii,² K. Tanabe.¹
¹Department of Urology, Tokyo Women's Medical University, Tokyo, Japan; ²Laboratory for Vaccine Design, RINEK Center for IMS, Yokohama, Japan.
We previously reported that combination therapy using liposomal α-galactosylceramide (lipo-αGC) and anti-CD154 mAb could expand regulatory T cells (Tregs) in vivo through activation of the…
2015 American Transplant Congress
Vascularized Bone Marrow Plays a Critical Role in Inducing Tolerance of Osteomyocutaneous Allografts
C.-H. Lin,¹ M. Anggelia,¹ Y.-L. Wang,¹ H.-Y. Cheng,¹ W. Lee,² G. Brandacher.²
¹Center for Vascularized Composite Allotransplantation, Chang Gung Memorial Hospital, Taipei, Taiwan; ²Department of Plastic and Reconstructive Surgery, Johns Hopkins University School of Medicine, Baltimore, MD.
Background: Vascularized composite allograft (VCA) that includes medullary bone may provide proposed benefits ofvascularized bone marrow transplantation (VBMT), such as a continuous supply of donor…
2015 American Transplant Congress
Effects of Late Kidney Graftectomy in Nonhuman Primates (NHPs) Tolerant of Co-Transplanted Heart and Kidney Allografts
M. Tonsho,¹ G. Benichou,¹ R. Smith,² R. Colvin,² J. Madsen.^1,3
¹MGH Transplant Center, Massachusetts General Hospital, Boston, MA; ²Pathology, Massachusetts General Hospital, Boston, MA; ³Cardiac Surgery, Massachusetts General Hospital, Boston, MA.
Co-transplantation of donor kidneys and bone marrow enables the induction of tolerance of allogeneic hearts in NHPs. We have previously demonstrated that the state of…
2015 American Transplant Congress
Combined Bone Marrow Plus Liver Transplantation in Cynomolgus Monkeys
J. Weiner, Y. Kato, R. Duran-Struuck, M. Martinez, S. Baker, P. Alonso, J. Zitsman, A. Wu, P. Houck, T. Pinyavat, J. Lefkowitch, S. Chaudhry, T. Kato, M. Sykes, A. Griesemer.
Columbia Center for Translational Immunology, Columbia University Medical Center, New York, NY.
IntroductionCombined kidney/bone marrow transplantation (CKBMT) induces tolerance in both primates and humans. Since liver is thought to be more tolerogenic than kidney, we hypothesized that…
2015 American Transplant Congress
Chimerism for Early Detection of Graft Versus Host Disease in Intestinal Transplantation
J. Garcia, T. Beduschi, P. Ruiz, A. Tekin, J. Fan, S. Nishida, G. Selvaggi, L. Cubley, R. Vianna.
Miami Transplant Institute, University of Miami Miller School of Medicine, Miami, FL.
Graft versus host disease (GVHD) is a potentially fatal complication after multivisceral transplantation (MVT). By definition, donor cells must be present in the blood of…