Articles tagged "Mixed chimerism"

2016 American Transplant Congress
Developing a PCR-Based System to Access the Correlation of Local Chimerism and Fate of the Vascularized Composite Allotransplant.
X.-T. Huang,¹ H.-Y. Cheng,¹ C.-F. Lin,¹ S.-S. Qiu,² L.-Y. Shih,¹ F.-C. Wei.^1,2,3
¹Center for Vascularized Composite Allotransplantation, Chang Gung Memorial Hospital, Gueishan, Taoyuan, Taiwan; ²Department of Plastic and Reconstructive Surgery, Chang Gung Memorial Hospital, Gueishan, Taoyuan, Taiwan; ³College of Medicine, Chang Gung University, Gueishan, Taoyuan, Taiwan.
It is established that development of mixed chimerism following allotransplantation is associated with induction of tolerance to allotransplants. Although chimerism in peripheral blood can be…
2016 American Transplant Congress
iNKT Cell Activation Under Co-Stimulatory Blockade Establishes Mixed Chimerism Through Thymic Regulatory T Cell Activation.
T. Hirai,¹ Y. Ishii,² R. Ishii,¹ S. Miyairi,¹ M. Ikemiyagi,¹ M. Okumi,¹ K. Tanabe.¹
¹Department of Urology, Tokyo Women's Medical University, Shinjuku, Tokyo, Japan; ²Laboratory for Vaccine Design, RIKEN-IMS, Yokohama, Kanagawa, Japan.
Background: Invariant natural killer T (iNKT) cells have recently garnered considerable attention for their potential to induce regulatory T cells (Tregs). We previously reported a…
2016 American Transplant Congress
Impact of Mixed Xenogeneic Chimerism on Human B Cell Response to Pig Antigens.
H. Li, S. Shao, P. Vishwasrao, M. Holzl, M. Sykes.
Columbia Center for Translational Immunology, Columbia University, New York, NY.
Xenotransplantation is a potential solution to the organ shortage in clinical transplantation. Pigs are regarded as the most suitable donors. Studies in pig-to-nonhuman primates have…
2016 American Transplant Congress
The Function of CD8⁺TCR^– Facilitating Cells and Regulatory T Cells Is Impaired in Flt3-Ligand Knockout Mice.
Y. Huang, H. Xu, T. Miller, Y. Wen, S. Ildstad.
Institute for Cellular Therapeutics, University of Louisville, Louisville, KY.
Graft CD8+TCR- facilitating cells (FC) enhance engraftment of hematopoietic stem cells (HSC) in allogeneic mouse recipients without causing graft-versus-host disease. FC induce antigen-specific CD4+CD25+FoxP3+ regulatory…
2015 American Transplant Congress
Rejection of an Allograft Through Non-MHC Antigens Does Not Translate to the Rejection of a Different Allograft from the Same Donor in Mixed Chimeras
K. Shinoda,¹ C. Chase,¹ P. Russell,¹ J. Madsen,¹ R. Colvin,² A. Alessandrini.¹
¹Transplant Center, Massachusetts General Hospital, Boston, MA; ²Pathology, Massachusetts General Hospital, Boston, MA.
We previously showed that tolerance, induced by mixed chimerism in mice, is antigen-specific: tolerance of heart and skin allografts is dependent on Foxp3+ cells that…
2015 American Transplant Congress
Regulatory T Cells Expanded by Activated iNKT Cells Facilitate Migration of Donor Dendritic Cells Into the Recipient Thymus and Subsequent Establishment of Clonal Deletion
T. Hirai,¹ R. Shibahara,¹ S. Miyairi,¹ M. Ikemiyagi,¹ K. Omoto,¹ Y. Ishii,² K. Tanabe.¹
¹Department of Urology, Tokyo Women's Medical University, Tokyo, Japan; ²Laboratory for Vaccine Design, RINEK Center for IMS, Yokohama, Japan.
We previously reported that combination therapy using liposomal α-galactosylceramide (lipo-αGC) and anti-CD154 mAb could expand regulatory T cells (Tregs) in vivo through activation of the…
2015 American Transplant Congress
Vascularized Bone Marrow Plays a Critical Role in Inducing Tolerance of Osteomyocutaneous Allografts
C.-H. Lin,¹ M. Anggelia,¹ Y.-L. Wang,¹ H.-Y. Cheng,¹ W. Lee,² G. Brandacher.²
¹Center for Vascularized Composite Allotransplantation, Chang Gung Memorial Hospital, Taipei, Taiwan; ²Department of Plastic and Reconstructive Surgery, Johns Hopkins University School of Medicine, Baltimore, MD.
Background: Vascularized composite allograft (VCA) that includes medullary bone may provide proposed benefits ofvascularized bone marrow transplantation (VBMT), such as a continuous supply of donor…
2015 American Transplant Congress
Effects of Late Kidney Graftectomy in Nonhuman Primates (NHPs) Tolerant of Co-Transplanted Heart and Kidney Allografts
M. Tonsho,¹ G. Benichou,¹ R. Smith,² R. Colvin,² J. Madsen.^1,3
¹MGH Transplant Center, Massachusetts General Hospital, Boston, MA; ²Pathology, Massachusetts General Hospital, Boston, MA; ³Cardiac Surgery, Massachusetts General Hospital, Boston, MA.
Co-transplantation of donor kidneys and bone marrow enables the induction of tolerance of allogeneic hearts in NHPs. We have previously demonstrated that the state of…
2015 American Transplant Congress
Combined Bone Marrow Plus Liver Transplantation in Cynomolgus Monkeys
J. Weiner, Y. Kato, R. Duran-Struuck, M. Martinez, S. Baker, P. Alonso, J. Zitsman, A. Wu, P. Houck, T. Pinyavat, J. Lefkowitch, S. Chaudhry, T. Kato, M. Sykes, A. Griesemer.
Columbia Center for Translational Immunology, Columbia University Medical Center, New York, NY.
IntroductionCombined kidney/bone marrow transplantation (CKBMT) induces tolerance in both primates and humans. Since liver is thought to be more tolerogenic than kidney, we hypothesized that…
2015 American Transplant Congress
Chimerism for Early Detection of Graft Versus Host Disease in Intestinal Transplantation
J. Garcia, T. Beduschi, P. Ruiz, A. Tekin, J. Fan, S. Nishida, G. Selvaggi, L. Cubley, R. Vianna.
Miami Transplant Institute, University of Miami Miller School of Medicine, Miami, FL.
Graft versus host disease (GVHD) is a potentially fatal complication after multivisceral transplantation (MVT). By definition, donor cells must be present in the blood of…