Articles tagged "Mice"

2022 American Transplant Congress
Non-Invasive Assessment of Hepatic Ischemia-Reperfusion Injury Using Dynamic Contrast Enhanced-MRI and T2 Mapping
O. C. Rodriguez¹, J. R. Liggett², J. Kang², K. Loh², Y. Lee¹, N. Sang¹, B. He¹, Y. Cui², K. Oza², D. Kwon³, B. Kallakury³, T. Fishbein², W. Cui², K. Khan², C. Albanese¹, A. Kroemer²
¹Lombardi Comprehensive Cancer Center and the Center for Translational Imaging, Georgetown University Medical Center, Washington, DC, ²MedStar Georgetown Transplant Institute, Washington, DC, ³Pathology, MedStar Georgetown University Hospital, Washington, DC
*Purpose: Hepatic ischemia-reperfusion injury (IRI) is a consequence of liver transplantation and hepatectomy that varies in severity and clinical significance. The diagnosis is made based…
2022 American Transplant Congress
Exosomal TGFbeta:LAP as Immunosuppressive Mediator of Allo-Tolerance
W. Burlingham¹, E. Jankowska-Gan², J. Fechner³, C. Little², J. Sullivan⁴, D. Foley¹
¹University of Wisconsin, Madison, WI, ²Surgery-Transplant Division, University of Wisconsin, Madison, WI, ³Surgery-Transplant Division, University of Wisconsin-Madison, Madison, WI, ⁴Surgery, University of Wisconsin, Madison, WI
*Purpose: TGFβ has long been known as a key component in tolerance mediated by Treg cells. Here we show that, unlike IL10, but like CD39/CD73,…
2022 American Transplant Congress
Autophagy is an Important Factor in the Development of Fibrosis Following Renal Injury; Implications for Post-Transplant IFTA
R. Lopez-Soler¹, X. Ding², Q. Zang³
¹Tansplant, Surgery, Edward Hines VA Medical Center, Hines, IL, ²Pathology, Loyola University Medical Center, Maywood, IL, ³Surgery, Loyola University Medical Center, Maywood, IL
*Purpose: Interstitial Fibrosis and Tubular Atrophy (IFTA) is the most common cause of long-term graft failure following renal transplant. There is currently no effective therapy.…
2022 American Transplant Congress
Protective Immunity Against a Murine Epstein-Barr Virus Homolog is Preserved During CD11b-CD154 Blockade
K. L. Alexander, D. Liu, M. L. Ford
Emory University Transplant Center, Emory University, Atlanta, GA
*Purpose: CD154 pathway antagonism has shown to be a promising target for inducing long-time graft survival, in some cases showing efficacy that is superior to…
2022 American Transplant Congress
Fibroblastic Reticular Cell Subset Transcriptional Reprogramming Differs in Response to Immunity versus Tolerance
L. Li¹, Y. Song¹, M. Shirkey¹, A. Kensiski¹, W. Piao¹, V. Saxena¹, S. Gavzy¹, B. Ma¹, R. Abdi², J. Bromberg¹
¹UMB, Baltimore, MD, ²Harvard University, Boston, MD
*Purpose: Lymph node (LN) fibroblastic reticular cells (FRCs) are central contributors to the LN niche. We hypothesized that FRC subsets reactive to immunity versus tolerance…
2022 American Transplant Congress
Contributions of Allogenicity and Antigen Presentation to Regulatory B Cell Suppression
K. Lee, F. Qiang, K. Deng, R. Matheson, J. Lei, C. LeGuern, J. Markmann
Center for Transplantation Sciences, Boston, MA
*Purpose: We previously generated in vitro Toll-like receptor (TLR)-activated B cells became regulatory B cells (Bregs) that suppress T cell proliferation and promote allograft tolerance.…
2022 American Transplant Congress
Rhesus-Primatized Mouse Model Generated Using Transgenic Immunodeficient Mice
C. Little¹, W. J. Haynes¹, L. Huang¹, C. Daffada¹, K. B. Wolfe², E. Perrin², J. A. Simpson¹, J. A. Kropp Schmidt², H. M. Hinkle², L. T. Keding², R. T. Behrens², D. T. Evans², D. Kaufman¹, J. A. Thomson², T. G. Golos², M. Brown¹
¹Surgery, University of Wisconsin, Madison, WI, ²University of Wisconsin, Madison, WI
*Purpose: To develop a translational primatized mouse model, akin to humanized mice, that allows for high-throughput in vivo experimentation to augment non-human primate pre-clinical studies.*Methods:…
2022 American Transplant Congress
Immunomodulatory Effects of Probiotic Bifidobacterium Bifidum with Tacrolimus and Sirolimus in Mouse Skin Graft Model
A. Han¹, C. Chung², H. Kim³, H. Ko⁴, E. Jo¹, S. Min¹, S. Kim⁵, H. Park⁵, J. Ha¹
¹Seoul National University College of Medicine, Seoul, Korea, Republic of, ²Inha University Hospital, Incheon, Korea, Republic of, ³Korea University Guro Hospital, Seoul, Korea, Republic of, ⁴Kyung Hee University Hospital, Seoul, Korea, Republic of, ⁵Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju, Korea, Republic of
*Purpose: Accumulating evidence suggests that gut microbiota actively crosstalks with the host immune system, and alterations of gut microbiota may exert immune regulatory effects. We…
2022 American Transplant Congress
Donor Kidney-Resident Passenger Macrophages Promote CCL8-CCR8 Mediated Early Allograft Inflammation
A. Dangi¹, I. Husain², N. Natesh³, X. Shen³, J. Kwun⁴, X. Luo⁵
¹Medicine, Duke University Medical Center, Durham, NC, ²Duke University Medical Center, Durham, NC, ³Biomedical Engineering, Duke University, Durham, NC, ⁴Duke Transplant Center, Durham, NC, ⁵Duke University School of Medicine, Durham, NC
*Purpose: Early intragraft infiltration of monocytes and macrophages has been associated with acute and chronic allograft rejections. Nevertheless, underlying mechanism remained poorly understood. In the…
2022 American Transplant Congress
A Novel Method for Ex Vivo Stimulation of Mesenchymal Stem Cells via Invariant Natural Killer T Cell Activation with ɑ-galactosylceramide
R. Ishii¹, E. Kawaguchi¹, T. Yamakawa¹, H. Ishigooka¹, T. Ikeda¹, R. Ishiyama¹, K. Saiga¹, T. Takagi¹, Y. Ishii², K. Tanabe¹
¹Tokyo Women's Medical University, Tokyo, Japan, ²Department of Immunological Diagnosis, Juntendo University Graduate School of Medicine, Tokyo, Japan
*Purpose: Mesenchymal stem cells (MSCs) typically exhibit unique regenerative and immunomodulatory properties when exposed to proinflammatory cytokines. Invariant natural killer T (iNKT) cells are known…