Articles tagged "Mice"

2022 American Transplant Congress
Infiltrating CD8+ Cytotoxic T Cells Are Converted to a Regulatory Phenotype in Accepted Kidney Allografts
T. Yokose¹, E. S. Szuter¹, M. T. Guinn¹, J. Ge², I. Rosales³, M. Ford⁴, P. S. Russell¹, A. G. Cuenca², J. C. Madsen¹, R. B. Colvin¹, A. Alessandrini¹
¹Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, ²Boston Children's Hospital, Boston, MA, ³Department of Pathology, Massachusetts General Hospital, Boston, MA, ⁴Department of Surgery, Emory University, Atlanta, GA
*Purpose: Mouse kidney allografts are spontaneously accepted in select, fully-mismatched donor-recipient strain combinations (i.e. DBA/2J to C57BL/6 (B6)) without any immunosuppressive drug treatment. We have…
2022 American Transplant Congress
Recipient Neutrophil Production of Myeloperoxidase is Required for NK Cell Activation During Acute Antibody Mediated Allograft Rejection
Y. Mitsui¹, D. Ueda¹, S. Miyairi¹, N. Dvorina¹, K. S. Keslar¹, A. Valujskikh¹, B. William¹, R. Fairchild²
¹Inflammation & Immunity, Cleveland Clinic, Cleveland, OH, ²Cleveland Clinic Foundation, Cleveland, OH
*Purpose: Dysregulated donor-specific antibody (DSA) responses are induced in B6.CCR5-/- mice transplanted with complete MHC mismatched A/J kidney allografts and are required for rejection of…
2022 American Transplant Congress
Pro- and Anti-Tolerogenic Microbiota Induce Distinct Gut Microbiome Metabolic Pathways and Loco-Regional Immune Responses
S. J. Gavzy¹, R. Lakhan², J. Iyyathurai², V. Saxena², Z. L. Lee², B. Ma², E. Mongodin², J. S. Bromberg²
¹University of Maryland School of Medicine, Baltimore, MD, ²University of Maryland, Baltimore, MD
*Purpose: Colitis-associated Desulfovibrio desulfuricans (Desulfo) induces pro-inflammatory cytokine responses in dendritic cell and macrophage cell lines as well as primary cells. In contrast, Bifidobacterium pseudolongum…
2022 American Transplant Congress
Bioink Based on the DECM for 3D-Bioprinting of Bionic Pancreas – First Results of Animal
M. Klak¹, K. Kosowska¹, T. Bryniarski¹, I. Lojszczyk¹, T. Dobrzanski², G. Tymicki¹, A. Filip¹, A. A. Szczepankiewicz³, R. Olkowski⁴, A. Kosowska⁴, A. Berman¹, A. Kaminski⁴, M. Wszola¹
¹Foundation of Research and Science Development, Warsaw, Poland, ²Polbionica LTD, Warsaw, Poland, ³Institute of Experimental Biology M. Nencki PAN, Warsaw, Poland, ⁴Medical University of Warsaw, Warsaw, Poland
*Purpose: Tissue engineering is an interdisciplinary field involving activities based on two interrelated areas: developing new methods to repair, regenerate and replace damaged tissues and…
2022 American Transplant Congress
P2X7R Inhibition Impairs In Vitro Training of Macrophages
H. H. Lancia¹, R. Sanchez², A. G. Lellouch¹, C. L. Cetrulo¹, J. Ochando³, G. Benichou⁴
¹Division of Plastic and Reconstructive Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA, ²Icahn School of Medicine at Mount Sinai, New York, NY, ³Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, ⁴Center for Transplantation Sciences, Department of Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA
*Purpose: Macrophage training and ATP-mediated P2X7R signaling are unrelated key elements of the innate immune response associated with allotransplant rejection. In the present study, we…
2022 American Transplant Congress
Type 1 Natural Killer T-cells: Key Mediators of Ischemia- Reperfusion Injury in Hepatic Steatosis
J. R. Liggett¹, J. Kang¹, K. Loh¹, K. Oza¹, Y. Cui¹, D. Kwon², H. Li³, B. Kallakury², T. Fishbein¹, W. Cui¹, K. Khalid¹, A. H. Kroemer¹
¹MedStar Georgetown Transplant Institute, Washington, DC, ²Pathology, MedStar Georgetown University Hospital, Washington, DC, ³Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC
*Purpose: Hepatic ischemia-reperfusion injury (IRI) poses a significant risk in liver transplantation and hepatic resection and is further exacerbated by hepatic steatosis. Type 1 Natural…
2022 American Transplant Congress
Portal Hypertension Inhibits the Antitumor Effect of Liver-Resident NK Cells Through IL-33 Signal
Y. Imaoka, M. Ohira, K. Sato, K. Imaoka, R. Nakano, Y. Tanaka, H. Ohdan
Hiroshima University, Hiroshima, Japan
*Purpose: Portal vein hypertension (PHT) after living donor liver transplantation (LDLT) is known to have associated with poor prognosis. Among patients with hepatocellular carcinoma (N=…
2022 American Transplant Congress
Non-Invasive Assessment of Hepatic Ischemia-Reperfusion Injury Using Dynamic Contrast Enhanced-MRI and T2 Mapping
O. C. Rodriguez¹, J. R. Liggett², J. Kang², K. Loh², Y. Lee¹, N. Sang¹, B. He¹, Y. Cui², K. Oza², D. Kwon³, B. Kallakury³, T. Fishbein², W. Cui², K. Khan², C. Albanese¹, A. Kroemer²
¹Lombardi Comprehensive Cancer Center and the Center for Translational Imaging, Georgetown University Medical Center, Washington, DC, ²MedStar Georgetown Transplant Institute, Washington, DC, ³Pathology, MedStar Georgetown University Hospital, Washington, DC
*Purpose: Hepatic ischemia-reperfusion injury (IRI) is a consequence of liver transplantation and hepatectomy that varies in severity and clinical significance. The diagnosis is made based…
2022 American Transplant Congress
Exosomal TGFbeta:LAP as Immunosuppressive Mediator of Allo-Tolerance
W. Burlingham¹, E. Jankowska-Gan², J. Fechner³, C. Little², J. Sullivan⁴, D. Foley¹
¹University of Wisconsin, Madison, WI, ²Surgery-Transplant Division, University of Wisconsin, Madison, WI, ³Surgery-Transplant Division, University of Wisconsin-Madison, Madison, WI, ⁴Surgery, University of Wisconsin, Madison, WI
*Purpose: TGFβ has long been known as a key component in tolerance mediated by Treg cells. Here we show that, unlike IL10, but like CD39/CD73,…
2022 American Transplant Congress
Autophagy is an Important Factor in the Development of Fibrosis Following Renal Injury; Implications for Post-Transplant IFTA
R. Lopez-Soler¹, X. Ding², Q. Zang³
¹Tansplant, Surgery, Edward Hines VA Medical Center, Hines, IL, ²Pathology, Loyola University Medical Center, Maywood, IL, ³Surgery, Loyola University Medical Center, Maywood, IL
*Purpose: Interstitial Fibrosis and Tubular Atrophy (IFTA) is the most common cause of long-term graft failure following renal transplant. There is currently no effective therapy.…