Articles tagged "Mice"

2018 American Transplant Congress
mTORC2 Deficiency in CD11c⁺dendritic Cells Promotes Acute Kidney Injury
H. Dai,^1,2 A. Watson,¹ D. Fantus,¹ L. Peng,² A. Thomson,¹ N. Rogers.¹
¹Starzl Transplantation Institute, University of Pittsburgh School of Medicine, Pittsburgh; ²Department of Urological Organ Transplantation, The Second Xiangya Hospital of Central South University, Changsha, China.
Introduction: The role of the mammalian/mechanistic target of rapamycin (mTOR) in the pathophysiology of renal ischemia-reperfusion injury (IRI) is poorly characterized; furthermore, the influence of…
2018 American Transplant Congress
Ablation of Both mTOR and STAT3 in T Cells Induces Heart Transplant Acceptance in Donor Skin-Sensitized Mice
A. Xie,^1,2 W. Chen.^2,3
¹Division of Cardiothoracic and Vascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China; ²Center for Immunobiology and Transplantation Research, Houston Methodist Research Institute, Houston, TX; ³Department of Surgery, Weill Cornell Medical College of Cornell University, New York, NY.
Allogeneic memory T cell response precludes the induction of transplant acceptance; how this process is regulated at the molecular level remains unclear. Both mTOR and…
2018 American Transplant Congress
NOS2 Deficiency Limits Long Term Survival of Murine Kidney Allografts
I. Aljabban,^1,2 B. Jiang,¹ C. Yang,¹ T. O'Shea,¹ D. Ndishabandi,¹ R. White,¹ P. Russell,¹ R. Colvin,¹ J. Madsen,¹ A. Alessandrini.¹
¹Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA; ²Pennsylvania State University College of Medicine, Hershey, PA.
Nitric oxide (NO) is a small molecule derived from L-arginine that governs a myriad of physiologic processes including the antimicrobial and tumoricidal responses. Although NO…
2018 American Transplant Congress
Glycolytic Bias in mTORC2-Deficient Dendritic Cells Potentiates Antigen-Specific Immunity and Accelerates Graft Rejection
A. Watson,¹ H. Dai,¹ J. Diaz-Perez,² A. Menk,³ M. Killeen,² G. Delgoffe,³ A. Mathers,² A. Thomson.¹
¹Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA; ²Dermatology, University of Pittsburgh, Pittsburgh, PA; ³Cancer Institute, University of Pittsburgh, Pittsburgh, PA.
The mechanistic target of rapamycin (mTOR) is known to function in two complexes: mTOR complex 1 (mTORC1) and mTORC2. While the function of mTORC1 in…
2018 American Transplant Congress
Lymph Node Stromal Laminins Affect Graft Survival through Regulation of CD4 T Cell Proliferation and Migration
L. Li, T. Simon, W. Piao, Y. Xiong, V. Saxena, T. Zhang, C. Wagner, J. Bromberg.
University of Maryland, Baltimore, MD.
Background: Our prior studies demonstrated that tolerance and immunity are associated with differential expression of lymph node (LN) stromal laminins α4β1γ1 (411) and α5β1γ1 (511),…
2018 American Transplant Congress
In Vitro α-Galactosylceramide Stimulation Expands CD4⁺CD25⁺Foxp3⁻ Regulatory T Cell Precursors in Murine Thymocytes
H. Katsumata,^1,2 M. Ikemiyagi,¹ T. Kanzawa,¹ H. Fukuda,¹ R. Ishii,¹ K. Saiga,^1,3 M. Okumi,¹ Y. Ishii,⁴ K. Tanabe.¹
¹Urology, Tokyo Women's Medical University, Tokyo, Japan; ²Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan; ³Urology, Jyoban Hospital of Tokiwa Foundation, Fukushima, Japan; ⁴Laboratory for Immunotherapy, Center for Integrative Medical Sciences (IMS) RIKEN, Yokohama, Japan.
Background: We previously established mixed chimerism after invariant natural killer T-cell stimulation by administration of liposomal α-galactosylceramide (lipo-αGC) and costimulatory blockade, leading to regulatory T…
2018 American Transplant Congress
Origin and Traffic of Donor-Derived Extracellular Vesicles during Allo-Sensitization
F. Zeng,¹ W. Shufesky,¹ M. Yang,¹ Q. Liu,¹ A. Larregina,² A. Morelli.¹
¹T.E. Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA; ²Department of Dermatology, University of Pittsburgh, Pittsburgh, PA.
The idea that donor dendritic cells (DCs) transplanted with the grafts and mobilized to draining lymphoid tissues (dLTs) present donor MHC molecules to allo-reactive naïve…
2018 American Transplant Congress
Lymphotoxin Beta Receptor Modulation in Lymphatic Endothelial Cells Alters Regulatory T Cell Dynamics
V. Saxena, W. Piao, Y. Xiong, L. Li, C. Wagner, J. Bromberg.
U Maryland, Baltimore, MD.
Background:Lymphotoxin beta receptor (LTβR) mediated signaling in lymphatic endothelium cells (LEC) is important for migration of regulatory T cells (Treg) to afferent lymphatic vessels (LV)…
2018 American Transplant Congress
The LncRNA MALAT1 Induces Tolerogenic DCs and Antigen Specific Tregs Subsequently Mediating Cardiac Allograft Tolerance in Mice via MiR-155/DC-SIGN/IL10 Axis
M. Zhang,^1,2 J. Wu,^1,2 H. Zhang,^1,2 M. Liu,^1,2 Y. Zheng,^1,2 X. Jin,^1,2 S. Li,^1,2 Q. Zhao,^1,2 X. Liu,^1,2 Y. Sun,^1,2 B. Yu.^1,2
¹Department of Cardiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China; ²The Key Laboratory of Myocardial Ischemia, Harbin Medical University, Ministry of Education, Harbin, Heilongjiang, China.
Background: Tolerogenic dendritic cells(DCs) by shaping T cells immunity and inducing Tregs play critical roles in the induction of allograft tolerance in transplantation. Long noncoding…
2018 American Transplant Congress
In Vivo Imaging and Function of Renal Tertiary Lymphoid Organs
K. Abou-Daya, D. Zhao, A. Williams, M. Oberbarnscheidt.
Starzl Transplantation Institute, Pittsburgh.
Tertiary lymphoid organs (TLOs) are ectopic lymphoid structures that arise in non-lymphoid tissues in the setting of chronic inflammation such as organ transplantation. The function…