Articles tagged "knockout"

2015 American Transplant Congress
miR-155 Different Roles in UCB iTreg and nTreg
Y. Lu, L. Lu.
Liver Transplantation Center of First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.
OBJECTIVES:miR-155 has been associated with both innate and adaptive immune responses. SOCS1, which is involved in Treg survival and function, is a direct target of…
2015 American Transplant Congress
Macrophage Specific β-Catenin Controls ROCK/PTEN Function and Regulates Innate Immunity in Liver Inflammation
S. Yue,¹ X. Zhou,² M. Zhou,¹ Y. Zhai,¹ R. Busuttil,¹ Q. Ying,² J. Kupiec-Weglinski,¹ B. Ke.¹
¹Dumont-UCLA Transplant Center, Dept. of Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA; ²Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Dept. of Stem Cell Biology & Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA.
Background: It has been shown that β-catenin is an important regulator of cell development, regeneration, and carcinogenesis. Our previous studies demonstrated the key role of…
2015 American Transplant Congress
Leukocyte Associated Immunoglobulin-Like Receptor 1 inhibits Th1 But Enhances Th17 Responses
V. Agashe,¹ M. Keller,² L. Haynes,¹ E. Jankowska-Gan,¹ J. Sullivan,¹ J. Kernien,¹ H. Suzuki,³ D. Wilkes,⁴ W. Burlingham.¹
¹Surgery, UW Madison, Madison, WI; ²Tulane University, New Orleans, LA; ³Chiba Graduate School of Medicine, Chiba, Japan; ⁴Indiana University School of Medicine, Indianapolis, IN.
Background: Leukocyte Associated Immunoglobulin-like Receptor 1 (LAIR1), is a transmembrane receptor expressed by immune cells. It recognizes collagen repeats via its extracellular domain and has…
2015 American Transplant Congress
Eomes-Expressing CD8+ T Cells and Th17 Cells Mediate Costimulatory Blockade-Resistant Allograft Rejection in Mice
M. Sabet-Baktach,¹ E. Eggenhofer,¹ P. Renner,¹ M. Lantow,¹ H. Schlitt,¹ E. Geissler,¹ A. Kroemer.^1,2
¹Department of Surgery, University Hospital Regensburg, Regensburg, Germany; ²Georgetown Transplant Institute, Georgetown Transplant Institute, Washington, DC.
The etiology of costimulatory blockade-resistant allograft rejection remains unclear. Therefore, the purpose of this study was to evaluate the responsiveness of distinct CD4+ and CD8+…
2015 American Transplant Congress
miRNA-181 Promotes Graft Prolongation by Plasmacytoid Dendritic Cells By Increasing T Regulatory Cells and Decreasing B Cells as Revealed By Cytometry By Time-Of-Flight
A. Lau,^1,2 M. Vitalone,¹ X. Qu,¹ T. Shawler,¹ O. Martinez,¹ C. Esquivel,¹ S. Krams.¹
¹Surgery, Stanford University, Stanford, CA; ²Pediatrics, UCSF, San Francisco, CA.
Liver allografts are well tolerated and other solid organ allografts, when transplanted concurrently with livers, show improved outcomes. However, the mechanisms underlying “hepatic tolerance” have…
2015 American Transplant Congress
Blocking CD40/CD154 Co-Stimulation Prevents Acute Antibody-Mediated Renal Allograft Rejection Induced by Memory CD4 T Cells
V. Gorbacheva, R. Fan, A. Valujskikh.
Immunology, The Cleveland Clinic, Cleveland, OH.
Immunosuppression-resistant alloreactive memory CD4 T cells induce robust responses against organ transplants. In murine heart allograft recipients donor-reactive memory CD4 T cells provide help for…
2015 American Transplant Congress
Donor CD47 Plays a Critical Role in the Control of Alloreactive T Cell Activation Following Hepatocyte Transplantation
M. Zhang,^1,2 H. Wang,¹ N. Navarro-Alvarez,¹ Y. Zheng,² Y.-G. Yang.¹
¹Columbia Center for Translational Immunology, Columbia University Medical Center, New York, NY; ²First Hospital, Jilin University, Changchun, Jilin, China.
Background: The interspecies incompatibility of CD47 is an important mechanism triggering rejection of cellular xenografts, including both hematopoietic and non-hematopoietic cells, by recipient macrophages. Our…
2015 American Transplant Congress
Myeloid Cell-Specific Heme Oxygenase-1 Is Crucial for Hepatoprotection in Liver Ischemia-Reperfusion Injury
K. Nakamura,¹ M. Zhang,² B. Ke,¹ R. Busuttil,¹ J. Araujo,² J. Kupiec-Weglinski.¹
¹Surgery, Liver Transplant, University of California, Los Angeles, Los Angeles, CA; ²Medicine, Division of Cardiology, University of California, Los Angeles, Los Angeles, CA.
Background and Aim: Heme oxygenase-1 (HO-1), an anti-oxidant enzyme, is essential in tissue resistance against innate immunity driven ischemia-reperfusion injury (IRI). Our group was the…
2015 American Transplant Congress
Damage Associated Molecular Patterns (DAMPs)-Activated Neutrophil Extracellular Trap Formation Exacerbates Liver Ischemia/Reperfusion Injury
H. Huang, S. Tohme, A. Al-Khafaji, A. Tsung.
Department of Surgery, University of Pittsburgh, Pittsburgh, PA.
Liver ischemia/reperfusion (I/R) injury is a clinically important phenomenon encountered during transplantation that contributes to initial graft function and subsequent allograft rejection. Formation of neutrophil…
2015 American Transplant Congress
Interleukin-17 Deficiency Attenuates Renal Allograft Injury and Prolongs Survival in a Murine Transplantation Model
T. Kwan,^1,2 S. Chadban,^1,2 J. Ma,^1,2 S. Bao,^2,3 S. Alexander,^2,4 H. Wu.^1,2
¹Sydney Medical School, University of Sydney, Sydney, New South Wales, Australia; ²Renal Medicine, Royal Prince Alfred Hospital, Sydney, New South Wales, Australia; ³Discipline of Pathology, School of Medical Sciences, Sydney, New South Wales, Australia; ⁴Centre for Kidney Research, The Children's Hospital at Westmead, Sydney, New South Wales, Australia.
Aim:To investigate the role of IL-17 in allograft rejection using a murine model of fully MHC-mismatched renal transplantation.Methods:Life-sustaining kidney transplants were performed in nephrectomized mice:…