Articles tagged "knockout"

2016 American Transplant Congress
Complement Receptor C5aR2 Deletion in Kidney Transplantation in Mice Attenuates Renal Damage.
A. Thorenz,¹ R. Chen,¹ K. Hueper,² B. Hensen,² C. Klemann,³ M. Meier,⁴ A. Klos,⁵ F. Gueler.¹
¹Nephrology, Medical School, Hannover, Germany; ²Radiology, Medical School, Hannover, Germany; ³Paediatric Surgery, Medical School, Hannover, Germany; ⁴Central Animal Facility, Medical School, Hannover, Germany; ⁵Microbiology, Medical School, Hannover, Germany.
ObjectiveThe complement receptors C5aR1 and C5aR2 serve distinct roles in immune regulation. In kidney transplantation the early post-implant ischemia reperfusion injury (IRI) causes rapid complement…
2016 American Transplant Congress
Genetic TIM-4 Deletion Promotes Immunoregulatory Tissue-Resident Macrophage Survival and Lowers the Barrier to Cardiac Allograft Tolerance.
T. Thornley,¹ P. Kyriazis,¹ K. Agarwal,¹ Z. Fang,¹ L. Ma,¹ V. Chipashvili,¹ M. Koulmanda,^1,2 T. Strom.^1,2
¹Medicine, Harvard Medical School and Beth Israel Deaconess Medical Center, Boston, MA; ²Surgery, Harvard Medical School and Beth Israel Deaconess Medical Center, Boston, MA.
We report that the CD169+TIM-4+ tissue-resident macrophage (TRM) subset is the dominant tissue-resident leukocyte in skin, heart, and pancreas from C57BL/6 mice and skin and…
2016 American Transplant Congress
Absence of Receptors for C3a or C5a Prevent Alloreactive CD8 T Effector Cell (Teff) Expansion but Differentially Impact Memory (Tmem) Persistence Posttransplantation.
D. Mathern, P. Heeger.
Translational Transplant Research Center, Dept of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY.
As Tmem are known barriers to transplant survival and tolerance in animals and human transplant recipients, understanding factors that drive the formation and survival of…
2016 American Transplant Congress
Abrogating Donor-Derived Myd88 Signaling Impairs the Recruitment of Recipient Neutrophils and CD8 T Cells into Lung Allografts in Mice.
Z. Zheng,¹ J.-J. Wang,¹ X. Yeap,¹ X. Kang,¹ Y. Xie,¹ X. Luo,² Z. Zhang.¹
¹Comprehensive Transplant Center, Northwestern University Feinberg School of Medicine, Chicago, IL; ²Division of Nephrology and Hypertension, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL.
Objectives: Transplant ischemia reperfusion injury (IRI) mediated primary graft dysfunction (PGD) remains the predominant cause of short- and long-term graft loss in lung transplantation. This…
2016 American Transplant Congress
TGFb in Regulatory B Cells-Dependent Tolerance.
H. Deirawan, L. Kojima, L. Washburn, J. Markmann, J. Kim.
Surgery, Massachusetts General Hospital, Boston, MA.
In transplantation, increasing evidence support that B cells can play a major regulatory role in addition to their critical functions in allo-immunity and graft rejection.…
2016 American Transplant Congress
mTORC2 Negatively Regulates Dendritic Cell (DC) Metabolism and the Allostimulatory Function of Donor DCs in Organ Transplantation.
A. Watson,^1,2 D. Raïche-Regué,¹ H. Dai,¹ K. Fabian,^3,4 G. Delgoffe,^4,5 W. Storkus,^3,4 A. Thomson.^1,2
¹Starzl Transplantation Institute, Univ. of Pittsburgh, Pittsburgh, PA; ²Pathology, Univ. of Pittsburgh, Pittsburgh, PA; ³Dermatology, Univ. of Pittsburgh, Pittsburgh, PA; ⁴Immunology, Univ. of Pittsburgh, Pittsburgh, PA; ⁵Cancer Institute, Univ. of Pittsburgh, Pittsburgh, PA.
The mechanistic target of rapamycin (mTOR) is a kinase that functions in at least two complexes: rapamycin (RAPA)-sensitive mTORC1 and RAPA-insensitive mTORC2. These complexes play…
2016 American Transplant Congress
Role of NRP1 in CD4+ T Cell Allo-Activation.
E. Lee, H. Kim, G. Demirci.
Department of Surgery, Boston Children's Hospital, Boston, MA.
Introduction: Neuropilin-1 (NRP1) is a transmembrane glycoprotein that functions as a co-receptor for class III/IV semaphorins, VEGF, and several other growth factors. NRP1 has been…
2016 American Transplant Congress
Gsk3β Regulates Macrophage Pro-Inflammatory Immune Activation via AMPK-Mediated Induction of Small Heterodimer Partner (SHP).
H. Zhou,^1,2 J. Zhu,^1,3 S. Yue,¹ R. Busuttil,¹ J. Kupiec-Weglinski,¹ X. Wang,² Y. Zhai.¹
¹Surgery, David Geffen School of Medicine-University of California-Los Angeles, LA, CA; ²Liver Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China; ³Liver Surgery, Renji Hospital, Shanghai, China.
Background & AimsAlthough glycogen synthase kinase 3 β (Gsk3β) has been found to play key roles in ischemia and reperfusion injuries (IRI) of different organs,…
2015 American Transplant Congress
Inflammasome Activation in Hepatocytes and Kupffer Cells Orchestrates the Hepatotoxic Effect of Bile Acids and Can Be Potential Target in the Management of Cholestatic Liver Injury
X. Liu,¹ T. Ma,¹ T. Billiar,² R. Tao.¹
¹Department of Hepatobiliary-Pancreatic Surgery, Zhejiang Provincial Peoples' Hospital, Hangzhou, Zhejiang Province, China; ²Department of Surgery, University of Pittsburgh Medical Center, Pittsburgh, PA.
Purpose: Cholestatic liver injury (CLI) is commonly associated with many hepatobiliary diseases and substantially increases the peri-operative morbidity and mortality in liver surgery and transplantation.…
2015 American Transplant Congress
Macrophage-Specific SIRT1 Regulates HO-1 Mediated Anti-Inflammatory and Cytoprotective Pathways
K. Nakamura,¹ M. Zhang,² B. Ke,¹ R. Busuttil,¹ J. Araujo,² J. Kupiec-Weglinski.¹
¹Surgery, Liver Transplant, University of California, Los Angeles, Los Angeles, CA; ²Medicine, Division of Cardiology, University of California, Los Angeles, Los Angeles, CA.
Background/Aim: Sirtuin1 (SIRT1), a NAD+-dependent type III histone/protein deacetylase, is essential for resistance against liver ischemia-reperfusion injury (IRI). We asked whether SIRT1 may affect cytoprotective…