Articles tagged "Genomics"

2017 American Transplant Congress
DNA Hypermethylation Caused by Ischemia in Kidney Transplants Predicts Future Chronic Injury to the Allograft.
L. Heylen,^1,2,3,4 B. Thienpont,^3,4 M. Naesens,^1,2 J. Depreeuw,^3,4 P. Busschaert,^3,4 D. Kuypers,^1,2 I. Jochmans,^2,5 D. Monbaliu,^2,5 J. Pirenne,^2,5 D. Lambrechts,^3,4 B. Sprangers.^1,2
¹Nephrology, University Hospitals Leuven, Leuven, Belgium; ²Microbiology and Immunology, KU Leuven, Leuven, Belgium; ³Oncology, KU Leuven, Leuven, Belgium; ⁴VIB, Leuven, Belgium; ⁵Abdominal Transplant Surgery, University Hospitals Leuven, Leuven, Belgium
Study Purpose: DNA demethylation results from hydroxymethylation and is inhibited by hypoxia. We investigated whether ischemia during kidney transplantation induces DNA methylation changes of the…
2016 American Transplant Congress
Early Post Renal Transplant aHUS in Patients with Heterozygous CFHR3-CFHR1 Deletion.
S. Rhazouani, A. Malone, T. Alhamad, T. Maw, D. Brennan, R. Delos Santos.
Renal Division, Washington University, Saint Louis, MO.
Background: Atypical hemolytic uremic syndrome (aHUS) is a rare disease with a triad of microangiopathic hemolytic anemia, thrombocytopenia, and acute renal failure. Subjects homozygous for…
2016 American Transplant Congress
Molecular Assessment of Kidney Transplant Biopsies Performs Similarly in Medulla and Cortex.
K. Madill-Thomsen,¹ J. Reeve,¹ G. Bohmig,² F. Eskandary,² P. Halloran.^1,3
¹Alberta Transplant Applied Genomics Center, Edmonton, AB, Canada; ²Department of Nephrology and Dialysis, Medical University of Vienna, Vienna, Austria; ³Department of Medicine, Division of Nephrology and Transplant Immunology, University of Alberta, Edmonton, AB, Canada.
Histologic assessment of kidney biopsies is usually unable to interpret medulla, but molecular changes associated with rejection likely occur in both medulla and cortex. Analysis…
2016 American Transplant Congress
VDJ Immune Repertoire Sequencing Predicts Patients at Risk of Alloimmune Injury.
M. Sirota,¹ T. Sigdel,¹ S. Boyd,² A. Fire,² M. Sarwal.¹
¹Surgery and Bioinformatics, UCSF, San Francisco, CA; ²Genetics, Stanford University, Palo Alto, CA.
Background: The complex repertoire of immune receptors generated by B cells enables allo-recognition in the context of transplantation across HLA barriers, and is a major…
2016 American Transplant Congress
Analysis of T Cell Receptor Repertoire in Routine Renal Allograft Biopsy.
R. Reindl-Schwaighofer,¹ J. Weinberger,¹ R. Jimenez-Heredia,¹ J. Sunzenauer,¹ S. Schaller,² S. Winkler,² R. Oberbauer.¹
¹Department of Nephrology, Medical University of Vienna, Vienna, Austria; ²Bioinformatics Research Group, University of Applied Sciences Upper Austria, Hagenberg, Austria.
Introduction: Diversity and clonality of the T cell repertoire of circulating and tissue-resident T-cells can be assessed by sequencing of recombined T cell receptor (TCR)…
2016 American Transplant Congress
Exome Sequencing and HLA-Independent Prediction of Long-Term Kidney Allograft Function.
L. Mesnard,^1,3,6 T. Muthukumar,^2,9 M. Burbach,² C. Li,² H. Shang,⁴ D. Dadhania,^2,3 J. Lee,^2,3 V. Sharma,² J. Xiang,⁴ M. Carmagnat,⁵ C. Suberbielle,⁵ N. Ouali,⁶ E. Rondeau,^6,1 J. Friedewald,⁸ M. Abecassis,⁷ M. Suthanthiran,^2,9 F. Campagne.³
¹UMR1155, INSERM, Paris, France; ²Division of Nephrology and Hypertension, New York Presbyterian Hospital, New York; ³Department of Physiology and Biophysics, The HRH Prince Alwaleed Bin Talal Bin Abdulaziz Alsaud Institute for Computational Biomedicine, New York, NY; ⁴Genomics Core Facility, Weill Cornell Medical College, New York, NY; ⁵Laboratoire d'histocompatibilité Hôpital Saint Louis, APHP, Paris, France; ⁶Service des Urgences Néphrologiques et Transplantation Rénale, APHP, Paris, France; ⁷Comprehensive Transplant Center, Northwestern University Feinberg School of Medicine, Chicago, IL; ⁸Northwestern University Feinberg School of Medicine, Chicago, IL; ⁹Department of Transplantation Medicine, Weill Cornell Medical College, New York, NY.
Current strategies to improve graft outcome following kidney transplantation consider information at the HLA loci. We asked whether information outside of the HLA loci can…
2016 American Transplant Congress
Impact of Ethnicity on Tacrolimus Trough/Dose Ratios Following Solid Organ Transplantation.
A. Santeusanio, A. Owusu-Obeng, J. Jeff.
Mount Sinai Hospital, New York.
Objective: to assess tacrolimus dosing requirements based upon ethnicity in order to help guide future decisions regarding dosage initiation and titration among Hispanic and African…
2016 American Transplant Congress
A Genome Wide Association Study of New Onset Diabetes After Transplant in Kidney Transplantation.
A. Giri,¹ M. Sanders,² D. Velez Edwards,¹ T. Ikizler,³ D. Roden,³ K. Birdwell.³
¹Epidemiology, Vanderbilt University Medical Center, Nashville, TN; ²Medicine, University of Iowa, Iowa City, IA; ³Medicine, Vanderbilt University Medical Center, Nashville, TN.
Purpose: New-onset diabetes after transplantation (NODAT) is associated increased mortality. Using a DNA biobank linked to electronic medical records (EMR) at Vanderbilt University (BioVU), we…
2016 American Transplant Congress
Differences in Tacrolimus Troughs and Doses Between Caucasians and African Americans with Two CYP3A5 Loss-of-Function Alleles: Looking Beyond CYP3A5 Expression.
W. Oetting,¹ D. Schladt,² W. Guan,¹ R. Remmel,¹ C. Dorr,² K. Sanghavi,¹ A. Matas,¹ R. Mannon,³ A. Israni,² P. Jacobson,¹ For DeKAF Genomics,¹ GEN-03 Investigators.¹
¹University of Minnesota, Minneapolis, MN; ²Hennepin County Medical Center and Minneapolis Medical Research Foundation, Minneapolis, MN; ³University of Alabama, Birmingham, AL.
Higher tacrolimus (TAC) dose requirements and lower TAC troughs (TT) in African Americans (AA) relative to Caucasians (CA) have been presumed to be due to…
2016 American Transplant Congress
Development of a Sequencing-Based Cytomegalovirus UL55 Glycoprotein B (gB) Genotyping Assay.
S. Cowden, K. Grantham, I. Caton, J. Hester, M. Altrich, S. Kleiboeker.
Viracor-IBT Laboratories, Lee's Summit, MO.
Background: Human cytomegalovirus (hCMV) is an important agent of serious viral infection in the solid organ transplant and allogeneic hematopoietic stem cell transplant populations. Severe…