Articles tagged "Genomics"

2017 American Transplant Congress
Integrative Analysis of ExomeSeq and Gene Expression Data for Identification of Novel Variants Associated with Antibody-Mediated Rejection in Kidney Transplant.
S. Pineda,^1,2 T. Sigdel,² A. Jackson,³ M. Sirota,^1,2 M. Sarwal.²
¹Institute for Computational Health Science, University of California, San Francisco (UCSF), San Francisco, CA; ²Department of Surgery, University of California, San Francisco (UCSF), University of California, San Francisco (UCSF), CA; ³Department of Medicine, Johns Hopkins, Baltimore, MD
Background: The objective of this work is to characterize the impact of the donor-recipient (D/R) HLA and non-HLA mismatches on the risk of antibody-mediated rejection…
2017 American Transplant Congress
Current Practice and Ethical Dimensions of Implementing APOL1 Testing into Living Donor Evaluation: A National Survey.
E. Gordon,¹ C. Wicklund,¹ R. Sharp,² J. Lee,¹ C. Johnson,¹ J. Friedewald.¹
¹Northwestern University, Chicago; ²Mayo Clinic, Rochester
BackgroundAfrican American (AA) live kidney donors (LKDs) have an even greater risk of kidney failure than European American live donors. Apolipoprotein 1 (APOL1) gene variants…
2017 American Transplant Congress
DNA Hypermethylation Caused by Ischemia in Kidney Transplants Predicts Future Chronic Injury to the Allograft.
L. Heylen,^1,2,3,4 B. Thienpont,^3,4 M. Naesens,^1,2 J. Depreeuw,^3,4 P. Busschaert,^3,4 D. Kuypers,^1,2 I. Jochmans,^2,5 D. Monbaliu,^2,5 J. Pirenne,^2,5 D. Lambrechts,^3,4 B. Sprangers.^1,2
¹Nephrology, University Hospitals Leuven, Leuven, Belgium; ²Microbiology and Immunology, KU Leuven, Leuven, Belgium; ³Oncology, KU Leuven, Leuven, Belgium; ⁴VIB, Leuven, Belgium; ⁵Abdominal Transplant Surgery, University Hospitals Leuven, Leuven, Belgium
Study Purpose: DNA demethylation results from hydroxymethylation and is inhibited by hypoxia. We investigated whether ischemia during kidney transplantation induces DNA methylation changes of the…
2017 American Transplant Congress
Genome-Wide Genotyping in 1022 Heart Transplantation Recipients & 758 Donors Reveal Association with Acute Allograft Rejection.
J. van Setten,¹ B. Cole,² B. Chang,² N. de Jonge,¹ M. Holmes,² C. Baan,⁴ O. Manintveld,⁴ A. Peeters,⁴ F. Dominguez,⁵ K. Khush,³ P. Garcia-Pavia,⁵ M. Huibers,¹ R. de Weger,¹ J. Moore,² F. Asselbergs,¹ B. Keating.²
¹UMC Utrecht, Utrecht, Netherlands; ²University of Pennsylvania, Philadelphia; ³Stanford University, Stanford, CA; ⁴Erasmus MC, Rotterdam, Netherlands; ⁵Puerta de Hierro University Hospital, Madrid, Spain
Introduction: Acute rejection (AR) in the 1st year post-cardiac transplant remains a significant clinical issue, and predisposes recipients to subsequent chronic allograft vasculopathy. Genetic factors…
2017 American Transplant Congress
A Pilot Trial of Genotype-Guided Dosing versus Standard Clinical Dosing in Pediatric Solid Organ Transplants.
S. Min, T. Daljevic, M. Lafreniere-Roula, C. Manlhiot, N. Nalli, H. Grasemann, S. Schwartz, B. Kamath, V. Ng, R. Parekh, S. Mital.
The Hospital for Sick Children, Toronto, ON, Canada
Purpose: Tacrolimus pharmacokinetics are influenced by age and variation in the CYP3A5 gene with CYP3A5 expressors (AA/AG) being fast metabolizers. The purpose of this trial…
2017 American Transplant Congress
A Universal Strategy to Characterize Allograft Infiltrating Cells by Bioinformatics Deconvolution of Next Generation Sequencing Data.
G. Thareja,¹ H. Yang,² S. Hayat,¹ C. Li,² F. Mueller,² C. Snopkowski,² L. Perry,² M. Magruder,² J. Lee,² D. Dadhania,² K. Suhre,¹ M. Suthanthiran,² T. Muthukumar.²
¹Weill Cornell Medicine-Qatar, Doha, Qatar; ²Weill Cornell Medicine, New York
RNA sequencing characterizes mRNA transcriptome at an unprecedented level of precision. Because organ transplantation is a unique situation of mRNAs encoded by two (donor and…
2017 American Transplant Congress
Impact of Culture Expansion and Inflammatory Cytokine Challenge on DNA Methylation Profiles in MSC.
S. de Witte,¹ F. Peters,¹ A. Merino,¹ S. Korevaar,¹ S. Elliman,³ P. Newsome,² J. Meurs,¹ K. Boer,¹ C. Baan,¹ M. Hoogduijn.¹
¹Department of Internal Medicine, Erasmus MC, Rotterdam, Netherlands; ²Orbsen Therapeutics Ltd., Galway, Ireland; ³Department of NIHR Liver Biomedical Research Unit and Centre for Liver Research, University of Birmingham, Birmingham, United Kingdom
Mesenchymal stromal cells (MSC) are studied as possible immunotherapy after solid organ transplantation. However, to obtain sufficient numbers, cells need to be expanded in culture.…
2017 American Transplant Congress
Characterization of Human Plasma Cell Proteasome Adaptations to Proteasome Inhibitor Therapy: A Precision Medicine Approach to Overcome Drug Resistance in Desensitization.
J. Driscoll,¹ S. Tremblay,¹ P. Brailey,¹ A. Girnita,¹ R. Alloway,¹ B. Aronow,² H. Singh,² E. Woodle.²
¹U of Cincinnati, Cincinnati, OH; ²Cincinnati Children's, Cincinnati, OH
Purpose: Bone marrow niche-resident plasma cells (BMNRPCs) exhibit variable sensitivity to proteasome inhibitor (PI) therapy. Here, we characterized alterations in the gene and protein expression…
2017 American Transplant Congress
Personalized Tacrolimus (TAC) Dosing Using Genetic Variants in Caucasian Kidney Transplant (Tx) Recipients.
D. Margraf,¹ R. Brundage,¹ D. Schladt,² W. Guan,³ B. Wu,³ R. Remmel,⁴ C. Dorr,^2,7 D. Berglund,¹ R. Mannon,⁵ A. Matas,⁶ W. Oetting,¹ A. Israni,^2,7 P. Jacobson,¹ For the DeKAF Genomics Investigators.
¹Dept of Experimental and Clinical Pharmacology, Col of Pharmacy, Univ of Minnesota(UMN), Mpls, MN; ²Chronic Disease Research Group, MPLS Medical Research Foundation, Mpls, MN; ³Div of Biostatistics, School of Public Health, UMN, Mpls, MN; ⁴Dept of Med Chem, Col of Pharmacy, UMN, Mpls, MN; ⁵Dept of Nephrology, Univ of Alabama, Birmingham, AL; ⁶Div of Transplantation, Dept of Surgery, School of Medicine, UMN, Mpls, MN; ⁷Dept of Nephrology, Hennepin County Medical Center, School of Medicine, UMN, Mpls, MN
TAC is an immune suppressant with a narrow therapeutic index and high pharmacokinetic (PK) variability leading to uncertainty in blood concentrations. We previously developed an…
2017 American Transplant Congress
Immunosuppression and Graft Rejection in Living-Related HLA-Identical Renal Transplantation.
R. Ossman,¹ M. Jamme,¹ P. Galichon,¹ A. Hertig,¹ B. Moulin,² C. Legendre,³ E. Morelon,⁴ J.-L. Taupin,⁵ L. Rostaing,⁶ E. Rondeau.¹
¹Urgences Néphrologiques et Transplantation, Hôpital Tenon, RADOVFULL Study Group, Université Pierre et Marie Curie, Paris, France; ²Néphrologie et Transplantation, Hôpital Civil, Strasbourg, France; ³Néphrologie et Transplantation, Hôpital Necker-Enfants Malades, Université Paris Descartes, Paris, France; ⁴Néphrologie et Transplantation, Hôpital Edouard Herriot, Université de Lyon, Lyon, France; ⁵Laboratoire d'Histocompatibilité, Hôpital Saint-Louis, Université Paris Diderot, Paris, France; ⁶Néphrologie et Transplantation d'Organes, Université Paul Sabatier, Toulouse, France
Purpose: We aimed to describe the immunosuppressive regimens and graft rejection rates in living-related HLA-identical (LR HLAid) renal transplantation.Methods: We performed a retrospective multicenter analysis…