Articles tagged "Genomic markers"

2021 American Transplant Congress
Integrative Analyses of Circulating Small Rnas and Paired Kidney Graft Transcriptome in Transplant Glomerulopathy
C. Kuscu¹, K. Manjari², M. Akram³, C. Kuscu⁴, A. Wolen,⁴, A. Bajwa⁴, J. Eason⁴, D. G. Maluf⁵, V. Mas⁶, E. Akalin⁷
¹Surgery, James D Eason Transplant Institute, UTHSC, Memphis, TN, ²Department of Systems and Computational Biology, School of Life Sciences, University of Hyderabad,, Hyderabad,, India, ³Center for Biomedical Informatics, University of Tennessee Health Science Center, Memphis, TN, ⁴Surgery, James D Eason Transplant Institute, Memphis, TN, ⁵Surgery, Program in Transplantation, University of Maryland, Baltimore, MD, ⁶Surgery, Division Surgical Science, University of Maryland, Baltimore, MD, ⁷Medicine, Montefiore Medical Center, Abdominal Transplant Program, Albert Einstein College of Medicine,, NYC, NY
*Purpose: Transplant glomerulopathy (TG) develops through multiple mechanisms including donor-specific antibodies, T cells and innate immunity. We investigate the role of circulating small RNAs (sRNAs)…
2021 American Transplant Congress
Genetic versus Self-reported African Ancestry and Kidney Allograft Outcome: Analysis of Two Large Multiethnic Urban Transplant Cohorts
F. Zanoni¹, D. Neugut¹, S. Mohan¹, A. Gharavi¹, B. Keating², K. Kiryluk¹
¹Department of Medicine, Division of Nephrology, Columbia University, New York, NY, ²Department of Surgery, Division of Transplantation, University of Pennsylvania, Philadelphia, PA
*Purpose: African-American (AA) kidney transplant recipients (KTx) have higher risk of rejection and failure. However, it is unknown to what extent the inferior outcomes in…
2021 American Transplant Congress
Development and Validation of a Novel Peripheral Blood Based Gene Signature for Acute Rejection Following Renal Transplantation
D. Zhang, Y. Wang, X. Hu
Department of Urology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China
*Purpose: The aim of this study is to utilize advanced machine learning methods to construct a peripheral blood-based gene signature for acute rejection following kidney…
2021 American Transplant Congress
Identification of Distinct Blood Monocyte Phenotypes from Transplant Kidney Recipients Undergoing Cellular (TCMR) and Mixed Antibody Mediated Rejection (ABMR)
C. Macedo, E. Bailly, K. Louis, M. Lucas, A. Zeevi, P. Randhawa, D. Metes
Starzl Transplantation Institute, Pittsburgh, PA
*Purpose: Monocytes play an important role in immune defense by initiating adaptive immunity. They are heterogeneous, plastic, and present different subsets: classical (CD14++CD16-), intermediate (CD14+CD16+),…
2021 American Transplant Congress
Validation of a Novel Gene Expression Biomarker of Rejection Following Liver Transplantation
J. Levitsky¹, M. Kandpal¹, T. Whisenant², K. Guo¹, S. Kurian³, M. Abecassis⁴
¹Gastroenterology & Hepatology; Comprehensive Transplant Center, Northwestern University, Chicago, IL, ²University of California San Diego, San Diego, CA, ³Scripps Clinic, La Jolla, CA, ⁴University of Arizona College of Medicine Tucson, Tucson, AZ
*Purpose: Non-invasive biomarkers distinguishing early signs of immune activation vs. quiescence could more objectively guide immunosuppression management after liver transplantation (LT). Our aim was to…
2021 American Transplant Congress
The Role of Donor-derived Cell-free DNA to Detect Subclinical Acute Rejection in Kidney Allograft
S. Park¹, K. Guo², R. Heilman³, E. Poggio⁴, D. Taber⁵, C. Marsh⁶, S. Kurian⁷, S. Kleiboeker⁸, J. Weems⁹, J. Holman¹⁰, L. Zhao², R. Sinha⁸, S. Brietigam², C. Rebello², M. Abecassis¹¹, J. Friedewald¹
¹Transplant nephrology, Northwestern University, Chicago, IL, ²Feinberg School of Medicine, Northwestern University, Chicago, IL, ³Transplant nephrology, Mayo Clinic College of Medicine and Science, Phoenix, AZ, ⁴Department of Nephrology and Hypertension, Cleveland Clinic, Cleveland, OH, ⁵Division of Transplant Surgery, Medical University of South Carolina, Charleston, SC, ⁶Department of Medicine and Surgery, Scripps Clinic and Green Hospital, La Jolla, CA, ⁷Scripps Health, La Jolla, CA, ⁸Eurofins US Clinical Diagnostics, Lee's Summit, MO, ⁹Eurofins US Clinical Diagnostics, Lee’s Summit, MO, ¹⁰Transplant Genomics, Inc, Mansfield, MA, ¹¹University of Arizona College of Medicine, Northwestern University, Tucson, AZ
*Purpose: We hypothesized that donor-derived cell-free DNA (dd-cfDNA) could detect subclinical acute rejection (subAR).*Methods: 429 blood samples paired with surveillance kidney biopsies from 208 patients…
2021 American Transplant Congress
Dd-cfdna Can Guide Safe Reintroduction of Immunosuppression in Kidney Transplant Recipients with Covid-19
J. Miles¹, J. Leonard², V. Tatapudi², M. Fei¹, R. Montgomery², N. M. Ali²
¹CareDx, Brisbane, CA, ²Transplant Institute, NYU Langone Health, New York, NY
*Purpose: COVID-19 infection is associated with 25% mortality in kidney transplant recipients (KTRs). Reduction of anti-metabolite immunosuppressants during the acute COVID-19 illness is a common…
2021 American Transplant Congress
Cellular Landscape of Steatotic Livers Revealed by Single-nuclei Rna Sequencing
C. Kuscu¹, C. Kuscu¹, A. Shetty², E. Bardhi³, T. Rousselle⁴, J. Eason¹, M. Sraj⁵, D. Maluf⁶, V. Mas⁴
¹Surgery, James D Eason Transplant Institute, Memphis, TN, ²University of Maryland, Institute for Genome Sciences, Baltimore, MD, ³Program in Transplantation - University of Maryland, Baltimore, MD, ⁴Surgery, Division of Surgical Science, Baltimore, MD, ⁵Surgery, University of Maryland, Baltimore, MD, ⁶Surgery, Program in Transplantation - University of Maryland, Baltimore, MD
*Purpose: Hepatic IRI represents a barrier to the use of available organs for transplant, particularly fatty livers. We aimed to discern the cellular subsets and…
2021 American Transplant Congress
Association Between Donor-Recipient Genetic Matching and Acute Rejection in Kidney Transplantation
R. Cao¹, V. Arthur², J. Chen², B. Keating², C. Dorr³, D. Schladt³, G. Onyeaghala³, R. Mannon⁴, A. Matas¹, R. Remmel¹, N. Pankratz¹, B. Wu¹, W. Oetting¹, P. Jacobson¹, A. Israni³, W. Guan¹
¹University of Minnesota, Minneapolis, MN, ²University of Pennsylvania, Philadelphia, PA, ³Hennepin Healthcare Research Institute, Minneapolis, MN, ⁴University of Nebraska, Omaha, NE
*Purpose: In this study we carried out a genome-wide association study (GWAS) between donor-recipient matching scores at individual SNP level and Acute rejection (AR) in…
2021 American Transplant Congress
Remote Monitoring Using Mobile Phlebotomy and Donor-derived Cell-free DNA in Kidney Transplant Recipients During the Covid-19 Pandemic
N. Ali¹, J. Miles², V. Tatapudi¹, R. Montgomery¹
¹NYU Langone, New York, NY, ²Medical Science Liason, CareDx, Brisbane, CA
*Purpose: The rapid shift to telemedicine and remote monitoring of kidney transplant recipients (KTRs) during COVID-19 aimed to mitigate exposure risk for this vulnerable population.…