Articles tagged "Genomic markers"

2021 American Transplant Congress
Analysis of Single Cell RNA Sequencing Data to Define Biomarkers of Human Liver Immune Tolerance
B. Rocque¹, A. Barbetta¹, P. Singh¹, C. Goldbeck¹, D. G. Helou², E. Loh³, N. Ung⁴, J. S. Lee⁴, O. Akbari², J. Emamaullee¹
¹Surgery, University of Southern California Keck School of Medicine, Los Angeles, CA, ²Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA, ³Norris Medical Library, University of Southern California Keck School of Medicine, Los Angeles, CA, ⁴Ellison Institute, University of Southern California, Los Angeles, CA
*Purpose: The liver is unique in its ability to maintain immune homeostasis and promote operational tolerance following solid organ transplantation. Single-cell RNA sequencing (scRNA seq)…
2021 American Transplant Congress
Early Experience with Broad Renal Genetic Testing in an Academic Transplant Center
E. Tantisattamo¹, K. Brossart², N. Ioannou², S. McCormick², P. R. Billings², H. Tabriziani²
¹Division of Nephrology, Hypertension and Kidney Transplantation, Division of Nephrology, Hypertension and Kidney Transplantation, Department of Medicine, University of California Irvine School of Medicine, Orange, CA, ²Natera, Inc., San Carlos, CA
*Purpose: Genetic testing is an emerging tool in kidney transplantation (KT), particularly to assess risk of recurrent disease in patients with KT and for selection…
2021 American Transplant Congress
Detection of Rejection in Kidney Transplant Patients Using an Algorithm That Combines Donor Fraction and Absolute Donor-derived Cell-free DNA
S. Bunnapradist¹, E. Ahmed², M. Maninder², Z. P. Demko², P. R. Billings², H. Tabriziani², P. Gauthier³
¹David Geffen Sch of Med @ UCLA, Los Angeles, CA, ²Natera, Inc., San Carlos, CA, ³Natera, Inc., Los Angeles, CA
*Purpose: Donor-derived cell-free DNA (dd-cfDNA) in the plasma of renal allograft patients is a clinically validated biomarker for allograft injury and rejection. Several dd-cfDNA assays…
2021 American Transplant Congress
Identification of Molecular Markers for Liver Cirrhosis by Single-nucleus Rna Sequencing
C. Kuscu¹, C. Kuscu¹, M. Akram², A. Shetty³, D. G. Maluf⁴, J. Eason¹, V. Mas⁵
¹Surgery, James D Eason Transplat Institute, Memphis, TN, ²Surgery, Center for Biomedical Informatics, Memphis, TN, ³Surgery, Institute for Genome Sciences, School of Medicine, Baltimore, MD, ⁴Surgery, Program in Transplantation University of Maryland, Baltimore, MD, ⁵Surgery, Division of Surgical Science University of Maryland, Baltimore, MD
*Purpose: Non-alcoholic fatty liver disease (NAFLD) is characterized by metabolic syndrome and the accumulation of fat in the liver cells (hepatocytes) leading to inflammation and…
2021 American Transplant Congress
Integrative Analyses of Circulating Small Rnas and Paired Kidney Graft Transcriptome in Transplant Glomerulopathy
C. Kuscu¹, K. Manjari², M. Akram³, C. Kuscu⁴, A. Wolen,⁴, A. Bajwa⁴, J. Eason⁴, D. G. Maluf⁵, V. Mas⁶, E. Akalin⁷
¹Surgery, James D Eason Transplant Institute, UTHSC, Memphis, TN, ²Department of Systems and Computational Biology, School of Life Sciences, University of Hyderabad,, Hyderabad,, India, ³Center for Biomedical Informatics, University of Tennessee Health Science Center, Memphis, TN, ⁴Surgery, James D Eason Transplant Institute, Memphis, TN, ⁵Surgery, Program in Transplantation, University of Maryland, Baltimore, MD, ⁶Surgery, Division Surgical Science, University of Maryland, Baltimore, MD, ⁷Medicine, Montefiore Medical Center, Abdominal Transplant Program, Albert Einstein College of Medicine,, NYC, NY
*Purpose: Transplant glomerulopathy (TG) develops through multiple mechanisms including donor-specific antibodies, T cells and innate immunity. We investigate the role of circulating small RNAs (sRNAs)…
2021 American Transplant Congress
Genetic versus Self-reported African Ancestry and Kidney Allograft Outcome: Analysis of Two Large Multiethnic Urban Transplant Cohorts
F. Zanoni¹, D. Neugut¹, S. Mohan¹, A. Gharavi¹, B. Keating², K. Kiryluk¹
¹Department of Medicine, Division of Nephrology, Columbia University, New York, NY, ²Department of Surgery, Division of Transplantation, University of Pennsylvania, Philadelphia, PA
*Purpose: African-American (AA) kidney transplant recipients (KTx) have higher risk of rejection and failure. However, it is unknown to what extent the inferior outcomes in…
2021 American Transplant Congress
Development and Validation of a Novel Peripheral Blood Based Gene Signature for Acute Rejection Following Renal Transplantation
D. Zhang, Y. Wang, X. Hu
Department of Urology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China
*Purpose: The aim of this study is to utilize advanced machine learning methods to construct a peripheral blood-based gene signature for acute rejection following kidney…
2021 American Transplant Congress
Identification of Distinct Blood Monocyte Phenotypes from Transplant Kidney Recipients Undergoing Cellular (TCMR) and Mixed Antibody Mediated Rejection (ABMR)
C. Macedo, E. Bailly, K. Louis, M. Lucas, A. Zeevi, P. Randhawa, D. Metes
Starzl Transplantation Institute, Pittsburgh, PA
*Purpose: Monocytes play an important role in immune defense by initiating adaptive immunity. They are heterogeneous, plastic, and present different subsets: classical (CD14++CD16-), intermediate (CD14+CD16+),…
2021 American Transplant Congress
Validation of a Novel Gene Expression Biomarker of Rejection Following Liver Transplantation
J. Levitsky¹, M. Kandpal¹, T. Whisenant², K. Guo¹, S. Kurian³, M. Abecassis⁴
¹Gastroenterology & Hepatology; Comprehensive Transplant Center, Northwestern University, Chicago, IL, ²University of California San Diego, San Diego, CA, ³Scripps Clinic, La Jolla, CA, ⁴University of Arizona College of Medicine Tucson, Tucson, AZ
*Purpose: Non-invasive biomarkers distinguishing early signs of immune activation vs. quiescence could more objectively guide immunosuppression management after liver transplantation (LT). Our aim was to…
2021 American Transplant Congress
The Role of Donor-derived Cell-free DNA to Detect Subclinical Acute Rejection in Kidney Allograft
S. Park¹, K. Guo², R. Heilman³, E. Poggio⁴, D. Taber⁵, C. Marsh⁶, S. Kurian⁷, S. Kleiboeker⁸, J. Weems⁹, J. Holman¹⁰, L. Zhao², R. Sinha⁸, S. Brietigam², C. Rebello², M. Abecassis¹¹, J. Friedewald¹
¹Transplant nephrology, Northwestern University, Chicago, IL, ²Feinberg School of Medicine, Northwestern University, Chicago, IL, ³Transplant nephrology, Mayo Clinic College of Medicine and Science, Phoenix, AZ, ⁴Department of Nephrology and Hypertension, Cleveland Clinic, Cleveland, OH, ⁵Division of Transplant Surgery, Medical University of South Carolina, Charleston, SC, ⁶Department of Medicine and Surgery, Scripps Clinic and Green Hospital, La Jolla, CA, ⁷Scripps Health, La Jolla, CA, ⁸Eurofins US Clinical Diagnostics, Lee's Summit, MO, ⁹Eurofins US Clinical Diagnostics, Lee’s Summit, MO, ¹⁰Transplant Genomics, Inc, Mansfield, MA, ¹¹University of Arizona College of Medicine, Northwestern University, Tucson, AZ
*Purpose: We hypothesized that donor-derived cell-free DNA (dd-cfDNA) could detect subclinical acute rejection (subAR).*Methods: 429 blood samples paired with surveillance kidney biopsies from 208 patients…