Articles tagged "Genomic markers"

2021 American Transplant Congress
Validation of a Novel Gene Expression Biomarker of Rejection Following Liver Transplantation
J. Levitsky¹, M. Kandpal¹, T. Whisenant², K. Guo¹, S. Kurian³, M. Abecassis⁴
¹Gastroenterology & Hepatology; Comprehensive Transplant Center, Northwestern University, Chicago, IL, ²University of California San Diego, San Diego, CA, ³Scripps Clinic, La Jolla, CA, ⁴University of Arizona College of Medicine Tucson, Tucson, AZ
*Purpose: Non-invasive biomarkers distinguishing early signs of immune activation vs. quiescence could more objectively guide immunosuppression management after liver transplantation (LT). Our aim was to…
2021 American Transplant Congress
The Role of Donor-derived Cell-free DNA to Detect Subclinical Acute Rejection in Kidney Allograft
S. Park¹, K. Guo², R. Heilman³, E. Poggio⁴, D. Taber⁵, C. Marsh⁶, S. Kurian⁷, S. Kleiboeker⁸, J. Weems⁹, J. Holman¹⁰, L. Zhao², R. Sinha⁸, S. Brietigam², C. Rebello², M. Abecassis¹¹, J. Friedewald¹
¹Transplant nephrology, Northwestern University, Chicago, IL, ²Feinberg School of Medicine, Northwestern University, Chicago, IL, ³Transplant nephrology, Mayo Clinic College of Medicine and Science, Phoenix, AZ, ⁴Department of Nephrology and Hypertension, Cleveland Clinic, Cleveland, OH, ⁵Division of Transplant Surgery, Medical University of South Carolina, Charleston, SC, ⁶Department of Medicine and Surgery, Scripps Clinic and Green Hospital, La Jolla, CA, ⁷Scripps Health, La Jolla, CA, ⁸Eurofins US Clinical Diagnostics, Lee's Summit, MO, ⁹Eurofins US Clinical Diagnostics, Lee’s Summit, MO, ¹⁰Transplant Genomics, Inc, Mansfield, MA, ¹¹University of Arizona College of Medicine, Northwestern University, Tucson, AZ
*Purpose: We hypothesized that donor-derived cell-free DNA (dd-cfDNA) could detect subclinical acute rejection (subAR).*Methods: 429 blood samples paired with surveillance kidney biopsies from 208 patients…
2021 American Transplant Congress
Dd-cfdna Can Guide Safe Reintroduction of Immunosuppression in Kidney Transplant Recipients with Covid-19
J. Miles¹, J. Leonard², V. Tatapudi², M. Fei¹, R. Montgomery², N. M. Ali²
¹CareDx, Brisbane, CA, ²Transplant Institute, NYU Langone Health, New York, NY
*Purpose: COVID-19 infection is associated with 25% mortality in kidney transplant recipients (KTRs). Reduction of anti-metabolite immunosuppressants during the acute COVID-19 illness is a common…
2021 American Transplant Congress
Cellular Landscape of Steatotic Livers Revealed by Single-nuclei Rna Sequencing
C. Kuscu¹, C. Kuscu¹, A. Shetty², E. Bardhi³, T. Rousselle⁴, J. Eason¹, M. Sraj⁵, D. Maluf⁶, V. Mas⁴
¹Surgery, James D Eason Transplant Institute, Memphis, TN, ²University of Maryland, Institute for Genome Sciences, Baltimore, MD, ³Program in Transplantation - University of Maryland, Baltimore, MD, ⁴Surgery, Division of Surgical Science, Baltimore, MD, ⁵Surgery, University of Maryland, Baltimore, MD, ⁶Surgery, Program in Transplantation - University of Maryland, Baltimore, MD
*Purpose: Hepatic IRI represents a barrier to the use of available organs for transplant, particularly fatty livers. We aimed to discern the cellular subsets and…
2021 American Transplant Congress
Association Between Donor-Recipient Genetic Matching and Acute Rejection in Kidney Transplantation
R. Cao¹, V. Arthur², J. Chen², B. Keating², C. Dorr³, D. Schladt³, G. Onyeaghala³, R. Mannon⁴, A. Matas¹, R. Remmel¹, N. Pankratz¹, B. Wu¹, W. Oetting¹, P. Jacobson¹, A. Israni³, W. Guan¹
¹University of Minnesota, Minneapolis, MN, ²University of Pennsylvania, Philadelphia, PA, ³Hennepin Healthcare Research Institute, Minneapolis, MN, ⁴University of Nebraska, Omaha, NE
*Purpose: In this study we carried out a genome-wide association study (GWAS) between donor-recipient matching scores at individual SNP level and Acute rejection (AR) in…
2021 American Transplant Congress
Remote Monitoring Using Mobile Phlebotomy and Donor-derived Cell-free DNA in Kidney Transplant Recipients During the Covid-19 Pandemic
N. Ali¹, J. Miles², V. Tatapudi¹, R. Montgomery¹
¹NYU Langone, New York, NY, ²Medical Science Liason, CareDx, Brisbane, CA
*Purpose: The rapid shift to telemedicine and remote monitoring of kidney transplant recipients (KTRs) during COVID-19 aimed to mitigate exposure risk for this vulnerable population.…
2021 American Transplant Congress
Urinary Exosome Mrna Signature for the Diagnosis of Human Kidney Transplant Rejection
R. El Fekih¹, J. Hurley², V. Tadigotla², A. Alghamdi³, A. Srivastava⁴, C. Coticchia², J. Choi¹, H. Allos⁵, k. yatim⁵, J. Alhaddad⁵, S. Eskandari⁵, P. Chu⁵, A. Mihali⁵, I. Lape⁵, M. Lima Filho⁵, B. Ayoma⁵, A. Chandraker⁵, K. Safa⁶, J. Markmann⁶, L. Riella⁶, R. Formica⁷, J. Skog², J. Azzi⁵
¹Medecine, Brigham and Women Hospital, Boston, MA, ²Exosome Diagnostics, Boston, MA, ³Boston Children Hospital, Boston, MA, ⁴Center for Translational Metabolism and Health, Chicago, IL, ⁵Transplantation Research Center, Boston, MA, ⁶Massachusetts General Hospital, Boston, MA, ⁷Yale School of Medicine, New Haven, CT
*Purpose: Traditional biomarkers currently used to monitor the kidney allograft, such as creatinine and proteinuria, are late markers of injury and lack sensitivity, specificity and…
2021 American Transplant Congress
Blood Gene Expression and Donor-derived Cell Free DNA for Diagnosing Subclinical Acute Rejection in Stable Kidney Transplant Recipients
S. Park¹, K. Guo², H. Raymond³, E. Poggio⁴, D. Taber⁵, C. Marsh⁶, S. Kurian⁷, S. Kleiboeker⁸, J. Weems⁸, J. Holman⁹, L. Zhao², R. Sinha⁸, S. Brietigam², C. Rebello², M. Abecassis¹⁰, J. Friedewald¹
¹Transplant Nephrology, Northwestern University, Chicago, IL, ²Feinberg School of Medicine, Northwestern University, Chicago, IL, ³Mayo Clinic College of Medicine and Science, Phoenix, AZ, ⁴Department of Nephrology and Hypertension, Cleveland Clinic, Cleveland, OH, ⁵Division of Transplant Surgery, Medical University of South Carolina, Charleston, SC, ⁶Department of Medicine and Surgery, Scripps Clinic and Green Hospital, La Jolla, CA, ⁷Scripps Health, La Jolla, CA, ⁸Eurofins US Clinical Diagnostics, Lee's Summit, MO, ⁹Transplant Genomics, Inc, Mansfield, MA, ¹⁰University of Arizona College of Medicine, Tucson, AZ
*Purpose: A blood gene expression profile (GEP) has been established as a non-invasive screen for subclinical acute rejection (subAR). For patients with allograft dysfunction, donor-derived…
2021 American Transplant Congress
Does the Timing of Subclinical Acute Rejection After Kidney Transplant Matter?
P. Portocarrero¹, S. Park², K. Guo³, L. Zhao³, J. Friedewald²
¹Nephrology, Northwestern University, Chicago, IL, ²Transplant Nephrology, Northwestern University, Chicago, IL, ³Feinberg School of Medicine, Northwestern University, Chicago, IL
*Purpose: We hypothesized that the timing of subclinical acute rejection (subAR) diagnosed with biopsy, gene expression profile or donor-derived cell-free DNA is associated with poor…
2021 American Transplant Congress
Analysis of Single Cell RNA Sequencing Data to Define Biomarkers of Human Liver Immune Tolerance
B. Rocque¹, A. Barbetta¹, P. Singh¹, C. Goldbeck¹, D. G. Helou², E. Loh³, N. Ung⁴, J. S. Lee⁴, O. Akbari², J. Emamaullee¹
¹Surgery, University of Southern California Keck School of Medicine, Los Angeles, CA, ²Molecular Microbiology and Immunology, University of Southern California, Los Angeles, CA, ³Norris Medical Library, University of Southern California Keck School of Medicine, Los Angeles, CA, ⁴Ellison Institute, University of Southern California, Los Angeles, CA
*Purpose: The liver is unique in its ability to maintain immune homeostasis and promote operational tolerance following solid organ transplantation. Single-cell RNA sequencing (scRNA seq)…