ATC Abstracts

American Transplant Congress abstracts

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Articles tagged "Gene expression"

  • 2022 American Transplant Congress

    Correlation of Donor-Derived Cell-Free DNA with Histology and Molecular Diagnoses of T-cell Mediated Rejection in Kidney Transplant Biopsies

    D. Kumar1, I. Moinuddin1, L. Kamal1, M. Shinbashi1, N. Raju1, R. Minniti1, F. Moran1, P. Halloran2, G. Gupta1

    1Virginia Commonwealth University, Richmond, VA, 2University of Alberta, Edmonton, AB, Canada

    *Purpose: We have reported the correlation of donor-derived cell-free DNA (dd-cfDNA) with histologic and molecular diagnosis (MMDx) of kidney transplant biopsies (Gupta et al, Transplantation…
  • 2022 American Transplant Congress

    Association Between Allograft Gene Expression and Acute Cellular Rejection Grade in Heart Transplant Recipients

    A. Kao1, K. Haffey2, K. T. Gondi3, J. Linard2, C. Knutson2, K. Morgan2, L. Teterina2, S. Williams2, K. Oreschak4, H. Zhang4

    1St. Luke's Health System Kansas City Mid America Heart Institute, Kansas City, MO, 2St. Luke's, Kansas City, MO, 3University of Michigan, Ann Arbor, MI, 4CareDx, South San Francisco, CA

    *Purpose: Endomyocardial biopsy (EMB) remains the gold standard in acute rejection (AR) surveillance in heart transplantation (HT) despite interpretation subjectivity and low concordance rates between…
  • 2022 American Transplant Congress

    Trajectory Analysis Of Single Cell RNAseq Data Suggest Intrarenal Induction Of Tregs In Spontaneously Accepted Kidney Allografts

    M. Guinn1, E. S. Szuter1, T. Yokose1, J. Ge2, B. Kwon1, I. Rosales3, P. S. Russell1, A. Cuenca2, J. Madsen1, R. Colvin3, A. Alessandrini1

    1Department of Surgery, Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, 2Department of Surgery, Boston Children's Hospital, Boston, MA, 3Department of Pathology, Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA

    *Purpose: Kidney allografts transplanted across certain mouse strain combinations (e.g. DBA/2 to B6) are spontaneously accepted independent of the recipient’s thymus, spleen, and lymph nodes.…
  • 2022 American Transplant Congress

    Characterization of GZMB+ Bregs Transcriptome and Their Impact on Target Cells in Single-Cell RNA-seq

    N. Sailliet, H. Le Mai, A. Dupuy, F. Cynthia, M. Chesneau, J. Poschmann, R. Danger, S. Brouard

    CRTI- INSERM UMR 1064, Nantes, France

    *Purpose: During the inflammatory process, the immune system capacity to regulate itself is mandatory to balance effector positive feedback, avoid allergies and autoimmunity. Different subsets…
  • 2022 American Transplant Congress

    Monitoring of Kidney Allograft Rejection Using Patient-Centric State-of-the-Art Tools – An Approach to Early Diagnosis and Intervention

    A. Rizvi1, A. Gamilla-Crudo2, S. A. Hussain3, M. Kueht4, J. Fair4, M. Mujtaba2

    1Internal Medicine, Nephrology, University of Texas Medical Branch, Galveston, TX, 2University of Texas Medical Branch, Galveston, TX, 3Nephrology, University of Texas Medical Branch, Houston, TX, 4Surgery, University of Texas Medical Branch, Galveston, TX

    *Purpose: The early detection and timely management of kidney allograft rejection using traditional markers including serum creatinine (SCr) has been inadequate due to lagging response.…
  • 2022 American Transplant Congress

    A Novel Prospective Validation Trial Of Multiple Blood-based RNA Signature Assays Pre And Post Kidney Transplant To Predict Rejection

    L. Gallon1, P. O'Connell2, A. Chang3, M. Donovan4

    1Northwestern Univ, Chicago, IL, 2Westmead Inst, Sydney, Australia, 3Univ of Chicago, Chicago, IL, 4Verici Dx, Franklin, TN

    *Purpose: The accuracy and utility of clinically defined and analytically validated predictive biomarkers in kidney transplantation is limited. We leveraged prior feasibility blood-RNA studies to…
  • 2022 American Transplant Congress

    Top Molecules Associated with CLAD in Lung Transplants Are Shared Between Mucosal Biopsies, Transbronchial Biopsies, and Kidney Transplant Biopsies at Risk of Progression

    M. Mackova1, K. Halloran2, P. F. Halloran1, .. and the INTERLUNG Study Group3

    1Alberta Transplant Applied Genomics Centre, Edmonton, AB, Canada, 2University of Alberta, Edmonton, AB, Canada, 3., ., AB, Canada

    *Purpose: Chronic lung allograft dysfunction (CLAD) is a physiologic state with grave consequences for progression to failure but cannot be diagnosed by biopsy histology. We…
  • 2022 American Transplant Congress

    Immunomodulation in Lung Transplant May Facilitate Transformation of Usual Interstitial Pneumonia Into Pleuroparenchymal Fibroelastosis

    Q. Cai1, K. Batra2, J. R. Torrealba1

    1Department of Pathology, The University of Texas Southwestern Medical Center, Dallas, TX, 2Department of Radiology, The University of Texas Southwestern Medical Center, Dallas, TX

    *Purpose: Pleuroparenchymal fibroelastosis (PPFE) is a rare but distinctive manifestation of interstitial lung disease (ILD) characterized by a highly elastotic pattern of lung fibrosis. Its…
  • 2022 American Transplant Congress

    Validation of Trugraf Gene Expression Profile in a Multicenter Cohort (TOGETHER Study)

    M. Mai1, W. Park2, R. Heilman3, B. Smith2, R. First4, J. Fleming4, P. West-Thielke4, J. Holman4, M. Stegall2

    1Mayo Clinic Jacksonville, Jacksonville, FL, 2Mayo, Rochester, MN, 3Mayo, Phoenix, AZ, 4Transplant Genomics, Framingham, MA

    *Purpose: The purpose of this study was to validate the performance characteristics of TruGraf GEP in a real-world population.*Methods: This was a multicenter observational analysis…
  • 2022 American Transplant Congress

    Leveraging Single-Cell Signatures in Kidney Transplant Biopsies Uncovers Immunological Heterogeneity Within Rejection Phenotypes

    J. Callemeyn1, B. Lamarthée1, P. Koshy2, T. Debyser3, E. Van Loon1, A. Franken4, T. Van Brussel4, I. Arijs4, D. Lambrechts4, M. Rabant5, A. Senev1, T. Vaulet6, C. Tinel1, M. Naesens1

    1Microbiology, Immunology and Transplantation, KU Leuven, Leuven, Belgium, 2Department of Morphology and Molecular Pathology, University Hospitals Leuven, Leuven, Belgium, 3Faculty of Medicine, KU Leuven, Leuven, Belgium, 4Laboratory for Translational Genetics, Department of Human Genetics, KU Leuven, Leuven, Belgium, 5Necker-Enfants Malades Institute, Institut national de la santé et de la recherche médicale (Inserm), Université de Paris, Paris, France, 6ESAT Stadius Center for Dynamical Systems, Signal Processing and Data Analytics, KU Leuven, Leuven, Belgium

    *Purpose: Rejection of the kidney allograft is hallmarked by the accumulation of inflammatory cells within the renal architecture, leading to microvascular or tubulointerstitial inflammation. However,…
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