Articles tagged "Gene expression"

2022 American Transplant Congress
Characterization of Hepatocytes During Cold Static Preservation at Single Cell Resolution
S. Naik¹, M. Dogan¹, C. Watkins¹, A. Bajwa¹, C. Eymard², J. Eason³, C. Kuscu¹, C. Kuscu¹
¹Transplant Research Institute, James D. Eason Transplant Institute, Department of Surgery, The University of Tennessee Health Science Center, Memphis, TN, ²James D. Eason Transplant Institute, Department of Surgery, The University of Tennessee Health Science Center, Memphis, TN, ³University of Tennessee/Methodist Transplant Institute, Memphis, TN
*Purpose: Hepatocytes together with liver sinusoidal endothelial cells (LSEC) are the most affected cells during cold preservation of donor liver. Necrotic and apoptotic hepatocytes release…
2022 American Transplant Congress
Association of Trugraf GEP with Clinical and Histological Outcomes in a Multicenter Cohort (TOGETHER Study)
R. Heilman¹, W. Park², M. Mai³, B. Smith², R. First⁴, J. Fleming⁴, P. West-Thielke⁴, J. Holman⁴, M. Stegall²
¹Mayo Clinic, Phoenix, AZ, ²Mayo Clinic, Rochester, MN, ³Mayo Clinic, Jacksonville, FL, ⁴Transplant Genomics, Framingham, MA
*Purpose: The purpose of this study was to validate the association of TruGraf GEP with clinical endpoints in a real-world cohort.*Methods: This was a multicenter…
2022 American Transplant Congress
Impact of Cytomegalovirus Infection on mRNA Expression Utilizing the Molecular Microscope in Heart Transplant Recipients
S. S. Patlolla, J. Van Zyl, K. Castro, S. Mckean, R. Patel, S. Carey, A. Alam, S. Hall
Cardiology, Baylor University Medical Center, Dallas, TX
*Purpose: Little is known about the effect of CMV infection on transplant messenger RNA expression utilizing the molecular microscope (MMDx) in the absence of acute…
2022 American Transplant Congress
Pretransplant Transcriptome Predicts 24-Month Kidney Function
E. Bardhi¹, K. Archer², D. Maluf¹, J. McDaniels¹, T. Rousselle¹, E. Akalin³, T. Mueller⁴, V. Mas¹
¹University of Maryland School of Medicine, Baltimore, MD, ²Ohio State University, Columbus, OH, ³Montefiore Medical Center, Bronx, NY, ⁴Universityspital Zurich, Zurich, Switzerland
*Purpose: With the development of novel prognostic tools derived from omics technologies, transplant medicine is entering the era of precision medicine, however, there are no…
2022 American Transplant Congress
Rejection After Liver Transplantation: What Can We Learn from Transcriptomic Analysis?
H. J. Braun¹, D. Amara², A. Zarinsefat¹, G. Szabo¹, Z. Laszik¹, P. Stock¹, N. Ascher¹
¹University of California, San Francisco, San Francisco, CA, ²UCLA, Los Angeles, CA
*Purpose: Acute cellular rejection (ACR) can occur at any time following liver transplantation (LT); initial ACR within 6 months is most common and occurs in…
2022 American Transplant Congress
TCMR in Late Kidney Transplant Biopsies Shows Less Molecular TCMR Activity and More Fibrosis Despite Persistence of Tubulitis and Interstitial Infiltrate
K. S. Madill-Thomsen¹, J. Reeve¹, P. Halloran¹, .. and the INTERCOMEX Study Group²
¹Alberta Transplant Applied Genomics Centre, Edmonton AB, AB, Canada, ²., ., AB, Canada
*Purpose: We studied the relationship between time post-transplant and molecular T cell-mediated rejection (TCMR) activity in a population of indication kidney transplant biopsies.*Methods: Updated rejection-based…
2022 American Transplant Congress
Biopsy and Blood Gene Expression Profiles Distinguish Injury, Subclinical and Clinical Rejection in Kidney Transplant Recipients
S. Kurian¹, M. Kandpal², C. Rebello², C. Marsh¹, J. Friedewald²
¹Scripps Health, La Jolla, CA, ²Northwestern University, Chicago, IL
*Purpose: We analyzed serial gene expression profiles in paired blood and biopsies from kidney transplant recipients phenotyped with a comprehensive clinical algorithm. We compared disease…
2022 American Transplant Congress
Increased Baboon Chimerism After Peripheral Blood Stem Cell Infusion from a Novel GalTKO Pig with Functional Humanized Il-3 Receptor
E. M. Duggan, N. P. Llore, J. M. Stern, H. Sakai, B. Piegari, K. Bruestle, D. Hajosi, B. Bhola, F. Huang, D. Ekanayake-Alper, D. H. Sachs, M. Sykes, R. J. Hawley, A. D. Griesemer
Columbia University Irving Medical Center, New York, NY
*Purpose: The purpose of this study was to investigate whether expression of a humanized IL-3 receptor (hCD123+hCD131) transgene in a GalTKO pig would improve levels…
2022 American Transplant Congress
Combined Genome Wide and Single Nuclei Transcriptomics in Posttransplant Acute Kidney Injury Identifies Early Proinflammatory/Profibrogenic Cell Origin
J. McDaniels¹, T. Rousselle¹, E. Bardhi¹, A. Shetty¹, E. Akalin², D. Maluf¹, V. Mas¹
¹University of Maryland, Baltimore, MD, ²Albert Einstein College of Medicine, Montefiore Medical Ctr, Bronx, NY
*Purpose: Kidney transplants offer a unique opportunity to evaluate molecular pathways involved in development and response to acute kidney injury (AKI). This prospective study of…
2022 American Transplant Congress
Immunometabolism of Regulatory T Cells Enhanced by Lipid Nanoparticles
J. S. Rink¹, Y. Sambandam², J. He², J. Mathew³, J. R. Leventhal⁴, C. S. Thaxton⁵
¹Department of Medicine, Division of Hematology/ Oncology, Northwestern University, Chicago, IL, ²Comprehensive Transplant Center, Northwestern University, Chicago, IL, ³Northwestern University, Chicago, IL, ⁴Surgery, Northwestern Medicine, Chicago, IL, ⁵Department of Urology, Northwestern University, Chicago, IL
*Purpose: Human regulatory T cell (Treg) metabolism is highly dependent upon a number of factors, including cholesterol obtained from circulating lipoproteins. We have developed high-density…