Articles tagged "Gene expression"

2016 American Transplant Congress
mRNA Expression of BAFF and APRIL Receptors Increases in Acute Rejection in Kidney Transplant Recipients.
M. Minz,¹ R. Dhital,¹ R. Minz,² A. Sharma,¹ R. Nada,³ S. Singh,¹ D. Kenwar.¹
¹Renal Transplant Surgery, Post Graduate Institute of Medical Education and Research, Chandigarh, India; ²Immunopathology, Post Graduate Institute of Medical Education and Research, Chandigarh, India; ³Histopathology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Introduction:BAFF and APRIL are known survival and growth of B- cells.BAFF and APRIL share two receptors i-TACI and ii- BCMA. Additionally, BAFF has strong affinity…
2016 American Transplant Congress
Inflammation Amplifier, a Chemokine Inducer in Non-Immune Cells in Kidney Transplantation Graft.
H. Higuchi,^1,2 H. Bando,² J. Jing-Jing,² T. Atsumi,² D. Iwami,¹ K. Morita,¹ N. Shinohara,¹ M. Murakami.²
¹Renal and Genitourinary Surgery, Hokkaido University, Sapporo, Japan; ²Molecular Neuroimmunology, Institute of Genetic Medicine, Graduate School of Medicine, Hokkaido University, Sapporo, Japan.
Introduction: Inflammation amplifier (IA), a local chemokine inducer in non-immune cells is induced by the simultaneous activation of NFκB and STATs (IL-17, TNFα, and IL-6)…
2016 American Transplant Congress
The Impact of Sequential Gene Modifications on Cardiac Xenograft Survival.
K. Horvath,¹ A. Singh,¹ J. Chan,¹ M. Thomas,² B. Lewis,² D. Ayares,³ P. Corcoran,¹ M. Mohiuddin.¹
¹CSRP, NHLBI/NIH, Bethesda, MD; ²DVR / ORS, NIH, Bethesda, MD; ³Revivicor, Inc, Blacksburg, VA.
Introduction: One of the major advances in xenotransplantation is the ability to modify donor pig genetics. Using rapidly evolving techniques of pig cloning and gene…
2015 American Transplant Congress
Small RNA Sequencing-Enabled Discovery of Circulating Extracellular MicroRNAs as Noninvasive Biomarkers of Liver Allograft Status
H. Yang,¹ T. Muthukumar,¹ K. Akat,² D. Briskin,² T. Tuschl,² M. Suthanthiran.¹
¹Weill Cornell Medical Center, New York; ²Rockefeller University, New York.
MicroRNAs are ideal candidates as blood-based biomarkers. Comprehensive profiling of microRNAs is challenging. We overcame the existing challenges by developing robust protocols for isolation of…
2015 American Transplant Congress
DNA Methylation of the IFNγ Promoter in CD8+ T Cell Subsets Is Modulated by CMV Infection but not by Alloreactivity in Kidney Transplantation Patients
K. Boer,¹ E. de Wit,¹ L. Hofland,² M. Betjes,¹ C. Baan.¹
¹Internal Medicine - Nephrology and Transplantation, Erasmus University Medical Center, Rotterdam, Netherlands; ²Internal Medicine - Endocrinology, Erasmus University Medical Center, Rotterdam, Netherlands.
Introduction DNA methylation plays a critical role in the function of cells, including cells of the immune system. Little is known about the methylation status…
2015 American Transplant Congress
Constitutively Upregulated Expression of Complement and Matrix Transcripts in Kidney Transplant Recipients Who Develop Acute Rejection
K. Keslar,¹ J. Reid-Adam,⁴ E. Kwon,⁴ D. Hricik,⁵ E. Poggio,^2,3 P. Heeger,⁴ R. Fairchild.^1,3
¹Immunology, Cleveland Clinic; ²Nephrology, Cleveland Clinic; ³Glickman Urological Institute and Transplant Center, Cleveland Clinic, Cleveland, OH; ⁴Icahn School of Medicine, Mount Sinai, New York, NY; ⁵Nephrology, UH Case Medical Center, Cleveland, OH.
Identification of kidney transplants at elevated risk of acute rejection (AR) and/or chronic injury prior to loss of kidney function (GFR) could guide therapies to…
2015 American Transplant Congress
Differentially Expressed IL-6Rα and GP130 Genes in Kidney Biopsies (Bxs) Suggests Different Roles of IL-6 Signaling in Antibody (ABMR)- and Cell (CMR)-Mediated Rejection
H. Zhang,¹ D. Thomas,¹ J. Kowalewska,² E. Suviolahti,¹ H. Shafi,² C. Nast,² A. Gertych,² A. Vo,¹ M. Haas,² S. Jordan,¹ M. Toyoda.¹
¹Transplant Immunology Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA; ²Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA.
Background: IL-6 exerts immunoregulatory properties through IL-6 receptors, IL-6Rα and gp130. Our previous studies suggested the involvement of antibody-dependent cellular cytotoxicity (ADCC) and its related…
2015 American Transplant Congress
Normothemic Machine Perfusion of Liver Grafts Promotes Liver Regeneration and Inhibits Tissue Inflammation
E. Xystrakis, J. Lozano, R. Danger, M. Martinez-Llordella, P. Srinivasan, N. Heaton, C. Coussios, A. Sanchez-Fueyo, Y. Ma, W. Jassem.
Institute of Liver Studies, King's College London School of Medicine at King's College Hospital, London, United Kingdom; Plataforma de Bioinformatica, Centro de Investigación Biomédica en red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Hospital Clinic, Centre Esther Koplovitz (CEK), Barcelona, Spain; Nuffield Department of Surgical Sciences, Oxford University, Oxford, United Kingdom.
Background & aim: liver transplantation I/R injury is associated with inflammation, affecting graft outcome. Ex-vivo normothermic machine perfusion (NMP) to donor livers before transplantation reduces…
2015 American Transplant Congress
OX40 Gene: A Potential Target for the Care of Chronic T-Cell Mediated Rejection (TCMR)
F. Schena,¹ C. Curci,¹ F. Sallustio,¹ M. Trpevski,¹ G. De Palma,¹ M. Fiorentino,² M. Rossini,² M. Quaglia,³ E. Bozzola,³ S. Zanini,⁴ L. Furian,⁴ A. Toscano,⁵ E. Gallo,⁶ L. Gesualdo,² P. Stratta,³ P. Rigotti,⁴ F. Citterio,⁵ L. Biancone.⁶
¹C.A.R.S.O. Consortium, Valenzano, Italy; ²University of Bari, Bari, Italy; ³A.O.U. Maggiore della Carità, Novara, Italy; ⁴University of Padova, Padua, Italy; ⁵Cattolica Sacro Cuore University, Rome, Italy; ⁶University of Torino, Turine, Italy.
Introduction:TCMR is characterized by the reduction of vessel lumen with marked intimal thickening, fibrous hyperplasia and a strong component of leukocyte infiltrate. Aim of our…
2015 American Transplant Congress
Epigenetic Reprogramming of Murine Cytomegalovirus Immediate Early Genes Induced By Renal Transplantation
M. Hummel,¹ X.-F. Liu,¹ C. Jie,¹ Z. Zhang,¹ S. Yan,¹ J.-J. Wang,¹ S. Kurian,² D. Salomon,² M. Abecassis.¹
¹Comprehensive Transplant Center, Northwestern University, Chicago, IL; ²Dept of Molecular and Experimental Medicine, The Scripps Research Institute, LaJolla, CA.
Background: Reactivation of latent CMV is a significant infectious complication in solid organ transplant recipients. MCMV is a valuable model for studying molecular mechanisms controlling…