ATC Abstracts

American Transplant Congress abstracts

  • Home
  • Meetings Archive
    • 2020 American Transplant Congress
    • 2019 American Transplant Congress
    • 2018 American Transplant Congress
    • 2017 American Transplant Congress
    • 2016 American Transplant Congress
    • 2015 American Transplant Congress
    • 2013 American Transplant Congress
  • Keyword Index
  • Resources
    • 2016 Resources
      • 2016 Welcome Letter
      • ATC 2016 Program Planning Committees
      • ASTS Council 2015-2016
      • AST Board of Directors 2015-2016
    • 2015 Resources
      • 2015 Welcome Letter
      • ATC 2015 Program Planning Committees
      • ASTS Council 2014-2015
      • AST Board of Directors 2014-2015
      • 2015 Conference Schedule
  • Advanced Search

mRNA Expression of BAFF and APRIL Receptors Increases in Acute Rejection in Kidney Transplant Recipients.

M. Minz,1 R. Dhital,1 R. Minz,2 A. Sharma,1 R. Nada,3 S. Singh,1 D. Kenwar.1

1Renal Transplant Surgery, Post Graduate Institute of Medical Education and Research, Chandigarh, India
2Immunopathology, Post Graduate Institute of Medical Education and Research, Chandigarh, India
3Histopathology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.

Meeting: 2016 American Transplant Congress

Abstract number: 134

Keywords: Gene expression, Kidney transplantation, Rejection

Session Information

Date: Sunday, June 12, 2016

Session Name: Concurrent Session: Kidney Immune Monitoring 1

Session Time: 4:30pm-6:00pm

 Presentation Time: 4:54pm-5:06pm

Location: Ballroom C

Related Abstracts
  • BAFF Binding to T Cell-Expressed BAFF-R Promotes Follicular Helper T Cell Proliferation and GC B Cell Responses
  • Distinct Clinical Relevance of BAFF Expression Within the Kidney Graft and Soluble BAFF Serum Levels Indicate Confined Alloimmune Responses Disconnected from the Periphery

Introduction:

BAFF and APRIL are known survival and growth of B- cells.

BAFF and APRIL share two receptors i-TACI and ii- BCMA. Additionally, BAFF has strong affinity to BAFF receptor (BAFF-R).

We intend to study the mRNA expression of BAFFR, TACI and BCMA in peripheral mononuclear cells of kidney transplant recipients during acute rejection.

Methodology:

Peripheral blood was collected from all patients before transplantation and were followed up for one year after transplantation. Blood samples were collected at 1 month, 3 months, 6 months, 12 months and at the time of rejection before initiation of anti- rejection therapy.

mRNA was isolated from PBMCs, converted to cDNA and stored at -80[deg]C. These cDNA were used to quantify mRNA expression level of BAFF-R, TACI and BCMA by Real time PCR (Light Cycler 480, Roche Diagnostics, Germany) using RT2 profiler PCR Array (CAPH12966F, QIAGEN). The expression level was calculated as fold change using the formula 2^(-[Delta][Delta]CT).

Percentage of CD20+ B- cells in peripheral circulation was calculated by flow cytometry. All the values are expressed as the mean ± standard error of mean (SEM). P value less than 0.05 was taken as statistically significant.

Result:

This study includes 76 first kidney transplant recipients who were divided into two groups: Rejection group (N=36) and Non Rejection group (NR, N=40).

Normalizing the pre- transplant gene expression level as 1, we found a significant rise in the gene expression level of BAFF-R (15.43 ± 4.469, P= 0.0027), TACI (5.425 ± 1.457, P= 0.0045) and BCMA (2.621 ± 0.7485, P= 0.0372) at the time of rejection. At the same time, we could also demonstrate a significant rise in peripheral percentage CD20+ B- cells during the rejection. we could not demonstrate significant changes in mRNA expression level of these genes in patients with stable graft function at post- 1 month, 3 month and 6 months of transplantation.

Conclusion:

There is an increase in mRNA expression of BAFFR, TACI and BCMA during rejection resulting in increase number of B- cells in peripheral blood. These molecular parameters might be used as predictive biomarker of acute rejection in Kidney transplant recipients.

CITATION INFORMATION: Minz M, Dhital R, Minz R, Sharma A, Nada R, Singh S, Kenwar D. mRNA Expression of BAFF and APRIL Receptors Increases in Acute Rejection in Kidney Transplant Recipients. Am J Transplant. 2016;16 (suppl 3).

  • Tweet
  • Email
  • Print

To cite this abstract in AMA style:

Minz M, Dhital R, Minz R, Sharma A, Nada R, Singh S, Kenwar D. mRNA Expression of BAFF and APRIL Receptors Increases in Acute Rejection in Kidney Transplant Recipients. [abstract]. Am J Transplant. 2016; 16 (suppl 3). https://atcmeetingabstracts.com/abstract/mrna-expression-of-baff-and-april-receptors-increases-in-acute-rejection-in-kidney-transplant-recipients/. Accessed March 7, 2021.

« Back to 2016 American Transplant Congress

Most Viewed Abstracts

  • This Week
  • This Month
  • All Time
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Penis Transplantation: First U.S. Experience.
  • Kidney Dialysis after Heart Transplantation: The Short and Long Term Outcomes
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease
  • Subtherapeutic Low Tacrolimus Trough Levels (≤3.5 Ng /ml) Are A Risk Factor For Acute Rejection And Creatinine Doubling.
  • Penis Transplantation: First U.S. Experience.
  • A Decade of Donor-Derived Disease: A Report of the OPTN Ad Hoc Disease Transmission Advisory Committee (DTAC).
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Home
  • Search
  • Penis Transplantation: First U.S. Experience.
  • Is There a Difference Between DCD and DBD Kidney Transplantation with Similar KDPI?
  • Low GFR after Kidney Donation Is Not Chronic Kidney Disease

Visit Our Partner Sites

American Transplant Congress (ATC)

Visit the official site for the American Transplant Congress »

American Journal of Transplantation

The official publication for the American Society of Transplantation (AST) and the American Society of Transplant Surgeons (ASTS) »

American Society of Transplantation (AST)

An organization of more than 3000 professionals dedicated to advancing the field of transplantation. »

American Society of Transplant Surgeons (ASTS)

The society represents approximately 1,800 professionals dedicated to excellence in transplantation surgery. »

Copyright © 2013-2021 by American Society of Transplantation and the American Society of Transplant Surgeons. All rights reserved.

Privacy Policy

loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.
This site uses cookies: Find out more.