Articles tagged "Gene expression"

2016 American Transplant Congress
Molecular Diagnosis of Antibody-Mediated Rejection in Routine Formalin-Fixed Paraffin-Embedded Nonhuman Primate Renal Allograft Biopsies.
B. Adam,¹ I. Rosales,² T. Oura,² R. Smith,² A. Cosimi,² T. Kawai,² R. Colvin,² M. Mengel.¹
¹University of Alberta, Edmonton, Canada; ²Harvard Medical School and Massachusetts General Hospital, Boston.
Background: In 2013, the Banff classification proposed molecular testing as an adjunct for the diagnosis of antibody-mediated rejection (ABMR) in renal allografts. We analyzed whether…
2016 American Transplant Congress
Predicting Delayed Kidney Graft Function with Gene Expression in Preimplantation Biopsies.
T. Mourão,^1,3 K. Mine,¹ E. Campos,^1,3 J. Medina-Pestana,^2,3 H. Tedesco-Silva,² M. Gerbase-De Lima.
¹Immunogenetics Institute, Associação Fundo de Incentivo à Pesquisa - AFIP, São Paulo, SP, Brazil; ²Hospital do Rim e Hipertensão, Fundação Oswaldo Ramos, São Paulo, SP, Brazil; ³UNIFESP, São Paulo, SP, Brazil.
Aiming to further the investigation of markers in pre-implantation biopsies (PIB) for the prediction of delayed graft function (DGF), we analyzed, in a different cohort…
2016 American Transplant Congress
Proteoforms: Novel Biomarkers for Acute Rejection in Liver Transplant Recipients.
J. Levitsky,¹ T. Toby,¹ K. Kim,¹ I. Ntai,¹ A. Demetris,² M. Abecassis,¹ P. Thomas,¹ N. Kelleher.¹
¹Northwestern, Chicago; ²UPMC, Pittsburgh.
Background: Biomarker profiles diagnostic of acute rejection (AR) in liver transplant (LT) recipients could enhance the diagnosis and management of recipients. Our aim was to…
2016 American Transplant Congress
Single Cell Profiling of Th9 Cells Allows Identification of Functionally Different Subsets.
K. Stanko, J. Schumann, K. Vogt, S. Schlickeiser, C. Appelt, B. Sawitzki.
Institute of Medical Immunology, Charite University Medicine, Berlin, Germany.
T helper type 9 (Th9) cells represent a distinct subset of CD4+ effector T cells that is characterized by high secretion of interleukin (IL)-9. Protective…
2016 American Transplant Congress
Assessment of mRNA Gene Profiles in Plasma Exosome to Predict Risk for Antibody-Mediated Rejection (ABMR) of Renal Allografts.
H. Zhang,¹ J. Kahwaji,¹ P. Li,² S. Ge,¹ D. Thomas,¹ C. Nast,³ A. Vo,¹ M. Haas,³ S. Jordan,¹ M. Toyoda.¹
¹Transplant Immunology Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA; ²Surgery, Cedars-Sinai Medical Center, Los Angeles, CA; ³Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA.
Background: ABMR is a major obstacle for successful transplant in HLA-sensitized (HS) patients. Complement-mediated and antibody-dependent cellular cytotoxicity (ADCC) are primarily mechanisms for ABMR. Previously…
2016 American Transplant Congress
Effect of Angiotensin Converting Enzyme Inhibitor (ACEI) or Angiotensin Receptor Blockade (ARB) Exposure on Intragraft Gene Expression of Kidney Transplant Patients.
C. Suarez-Fuentes,^1,2,4 P. O Broin,³ Y. Bao,¹ E. Akalin.^1,2
¹Kidney Transplant, Montefiore Medical Center, Bronx, NY; ²Albert Einstein College of Medicine, Bronx, NY; ³National University of Ireland Galway University, Galway, Ireland; ⁴Funded by NIH T-32 DK007110, National Institute of Health, Bethesda, MD.
Objective: Treatment with ACEI or ARB has been shown to have anti-inflammatory effects in animal models but this effect has not been investigated in kidney…
2016 American Transplant Congress
A Unique In Vitro Migration Model for Leukocyte Trans-Lymphatic Endothelial Migration.
Y. Xiong,¹ C. Brinkman,¹ K. Hippen,² B. Blazar,² J. Bromberg.¹
¹University of Maryland, Baltimore; ²University of Minnesota, Minneapolis.
Trans-endothelial migration (TEM) is an essential process for leukocyte circulation. While much is known about lymphocyte blood to tissue migration, far less is known about…
2016 American Transplant Congress
The Phenotypic Diversity of Late Silent Antibody-Mediated Rejection.
F. Eskandary,^1,2 J. Reeve,^1,3 H. Regele,⁴ L. Hidalgo,^1,3 D. Yoo,¹ N. Kozakowski,⁴ K. Famulski,¹ G. Böhmig,² P. Halloran.^1,5
¹Alberta Transplant Applied Genomics Centre, University of Alberta, Edmonton, AB, Canada; ²Division of Nephrology and Dialysis, Medical University of Vienna, Vienna, Austria; ³Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada; ⁴Department of Clinical Pathology, Medical University of Vienna, Vienna, Austria; ⁵Department of Medicine, Division of Nephrology and Transplant Immunology, University of Alberta, Edmonton, AB, Canada.
Study purpose: ABMR is a driving force of long-term allograft loss in organ transplantation. The present study was designed to characterize the phenotypic diversity of…
2016 American Transplant Congress
A Single Histone/protein Acetyltransferase, Tip60, Is Absolutely Essential for Treg Survival and Function, and Its Inhibition Abrogates Induction of Treg-Dependent Allograft Tolerance.
L. Wang,¹ A. Beeler,² S. Dahiya,¹ R. Han,¹ W. Hancock.¹
¹CHOP & UPenn, Philadelphia, PA; ²Boston University, Boston, MA.
Based on in vitro studies and limited in vivo data from patients with mutations in FOXP3 or animals with depletion of circulating Tregs, Tregs are…
2016 American Transplant Congress
Adiponectin Is Produced by Leukocytes Infiltrating Renal Transplants.
S. Goparaju, R. Fan, K. Keslar, N. Dvorina, W. Baldwin III.
Immunology, Cleveland Clinic, Cleveland, OH.
Fat is now recognized as a source of immunologically significant cytokines. Adiponectin, the most abundant adipokine, is usually classified as an anti-inflammatory cytokine because decreased…