Articles tagged "Gene expression"

2022 American Transplant Congress
Kidney Grafts with Poor 24-Month Function Present Upregulated Immune Activity at Pre-Transplantation
E. Bardhi¹, K. Archer², D. Maluf¹, J. McDaniels¹, T. Rousselle¹, E. Akalin³, T. Mueller⁴, V. Mas¹
¹University of Maryland, Baltimore, MD, ²Ohio State University, Columbus, OH, ³Montefiore Medical Center, Bronx, NY, ⁴University Hospital Zürich, Zürich, Switzerland
*Purpose: A transcriptomic profile serves as a snapshot of the temporary cell state and thus, analyzing pretransplant kidney biopsies can provide detailed information on the…
2022 American Transplant Congress
The Role Of Gut Microbiome In Optimum Dose Selection Of Tacrolimus For Kidney Transplant Recipients
M. Nayebpour¹, N. Koizumi¹, J. Melancon²
¹George Mason University, Arlington, VA, ²George Washington University Hospital, Washington, DC
*Purpose: This study aims to validate and apply a personalized optimal dose selection model for Tacrolimus (Tac) for kidney transplant recipients at the George Washington…
2022 American Transplant Congress
Gene Expression Changes Including Long Non-Coding RNAs in Human Hepatic Ischemia Reperfusion Injury
C. Kuscu¹, S. Naik¹, C. Eymard², H. Ingram¹, A. Bajwa¹, D. Maluf³, V. Mas⁴, J. Eason⁵, C. Kuscu¹
¹Transplant Research Institute, James D. Eason Transplant Institute, Department of Surgery, The University of Tennessee Health Science Center, Memphis, TN, ²James D. Eason Transplant Institute, Department of Surgery, The University of Tennessee Health Science Center, Memphis, TN, ³Surgery, University of Maryland, Baltimore, MD, ⁴University of Maryland, Baltimore, MD, ⁵University of Tennessee/Methodist Transplant Institute, Memphis, TN
*Purpose: Ischemia reperfusion injury (IRI) is still one of the major problems in liver transplantation affecting long term outcomes. This study is sought out to…
2022 American Transplant Congress
Single-Cell RNA Sequencing Identifies the Changes of Human Normal Kidney After Transplantation
G. Huang¹, H. Zhang¹, X. Chen¹, G. Zhao¹, J. Luo¹, S. Yang², J. Qiu¹
¹Organ Transplant Center, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China, ²Department of Pathology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China
*Purpose: We found that in all studies about kidney transplantation and single cell RNA sequencing (scRNA-seq), the native kidney rather than the transplanted kidney was…
2022 American Transplant Congress
A Signature of Tolerance Which Identifies Subclinical Rejection-Free Patients at One Year After Kidney Transplantation
R. Danger¹, L. Le Berre¹, M. Cadoux¹, C. Kerleau¹, P. Guérif¹, E. Morelon², O. Thaunat², C. Legendre³, D. Anglicheau³, K. Renaudin¹, M. Giral¹, S. Brouard¹
¹Centre de Recherche en Transplantation et Immunologie (CRTI), UMR 1064, ITUN, Nantes Université, CHU Nantes, Inserm, Nantes, France, ²Edouard Herriot Hospital, Hospices Civils de Lyon, Inserm U1111, Lyon-Est Medical Faculty, Claude Bernard University (Lyon 1), Lyon, France, ³APHP, Necker-Enfants Malades Institute, Inserm, Paris University, Paris, France
*Purpose: Spontaneous and rare cases of patients undergoing a functional renal transplant in the absence of immunosuppression represent a particular situation of immune tolerance. We…
2022 American Transplant Congress
Technology-Driven Performance Improvement of a Biomarker Panel in Kidney Transplant: OmniGraf
P. West-Thielke, C. Miller, N. Grund, A. Sutton, R. Sinha, M. Miller, S. Kleiboeker, J. Weems
Eurofins, Framingham, MA
*Purpose: Analyze the impact of the transition from TruGraf microarray to TruGraf PCR on the complementary performance characteristics of TruGraf plus TRAC (OmniGraf).*Methods: Development of…
2022 American Transplant Congress
Investigating RNA Interference-Mediated Inhibition of Liver Ischemia Reperfusion Injury During Liver Preservation
J. E. Buchwald¹, D. Echeverria², J. Sousa², N. McHugh², A. Khvorova², P. N. Martins³
¹RNA Therapeutics Institute, Department of Surgery, University of Massachusetts Chan Medical School, Worcester, MA, ²RNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA, ³Department of Surgery, University of Massachusetts Chan Medical School, Worcester, MA
*Purpose: Liver transplantation cures acute end-stage and chronic liver diseases, yet current strategies of organ preservation including static cold storage (SCS) and machine perfusion (MP)…
2022 American Transplant Congress
Gene Expression Profiles of Peritubular Capillarits in Chronic Antibody-Mediated Rejection
M. Eder¹, M. Röbl¹, H. Omic¹, B. Adam², D. Cejka³, N. Kozakowski⁴, D. D´Andrea⁵, M. Mengel², Ž. Kikić⁵
¹Internal Medicine III, Nephrology and Dialysis, Medical University of Vienna, Vienna, Austria, ²Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada, ³Department of Medicine III, Nephrology, Hypertension, Transplantation and Rheumatology, Ordensklinikum Linz – Elisabethinen, Linz, Austria, ⁴Department of Pathology, Medical University of Vienna, Vienna, Austria, ⁵Department of Urology, Medical University of Vienna, Vienna, Austria
*Purpose: Antibody-mediated rejection (ABMR) is one of the most significant contributors to late graft loss. Hallmark characteristics include peritubular capillaritis (ptc) and glomerulitis (g). Diffuse…
2022 American Transplant Congress
Crosstalk Between micro-RNAs, mRNAs, and Long Non-Coding RNAs in Human Hepatic Ischemia Reperfusion Injury
C. Kuscu¹, S. Naik¹, C. Eymard², A. Bajwa¹, D. Maluf³, V. Mas³, J. Eason⁴, C. Kuscu¹
¹Transplant Research Institute, James D. Eason Transplant Institute, Department of Surgery, The University of Tennessee Health Science Center, Memphis, TN, ²James D. Eason Transplant Institute, Department of Surgery, The University of Tennessee Health Science Center, Memphis, TN, ³Surgery, University of Maryland, Baltimore, MD, ⁴University of Tennessee/Methodist Transplant Institute, Memphis, TN
*Purpose: Present study utilizes multiple omics tools and clinical samples with a system biology approach characterizing mRNAs, long non-coding RNAs (lncRNAs) and small non-coding RNAs…
2022 American Transplant Congress
Evaluating Long Term Outcomes in Kidney Transplant Patients with TruGraf Gene Expression and TRAC Dd-cfdna in the TRULO Study
P. West-Thielke, W. Ally, J. Hickey, L. Stach, J. Fleming, T. Cober, A. Kawano, I. Agboli, C. Miller, J. Holman
Eurofins Transplant Genomics, Framingham, MA
*Purpose: The aim of this study is to evaluate long term clinical outcomes in kidney transplant patients undergoing non-invasive rejection monitoring with the OmniGraf® biomarker…