Articles tagged "Gene expression"

2018 American Transplant Congress
Time and Perfusate Dependent Molecular Response in Ex Vivo Lung Perfusion
P. Dromparis,¹ N. Aboelnazar,² S. Wagner,¹ S. Himmat,² C. White,² J. Luc,² D. Freed,² J. Nagendran,² M. Mengel,¹ B. Adam.¹
¹Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Canada; ²Department of Surgery (Cardiac), University of Alberta, Edmonton, Canada.
Lung transplantation has limited organ utilization and high rates of primary graft dysfunction, in part due to acute lung injury (ALI). Cold static preservation (CSP)…
2018 American Transplant Congress
Reduced Ratio of FoxP3/ RORγt Gene Expression, in Peripheral Blood, is Associated with Acute Allograft Rejection in Recipients with Kidney Transplantation
R. Dhital,^1,2 R. Minz,³ M. Minz,^1,4 A. Sharma,¹ R. Nada,⁵ S. Singh,¹ D. Kenwar,¹ S. Anand.³
¹Renal Transplant Surgery, Post Graduate Institute of Medical Education and Research, Chandigarh, UT, India; ²Vaccines and Immunity, Nationwide Children's Hospital, Columbus, OH; ³Immunopathology, Post Graduate Institute of Medical Education and Research, Chandigarh, UT, India; ⁴Organ Transplantation, Fortis Hospital, Mohali, Punjab, India; ⁵Histopathology, Post Graduate Institute of Medical Education and Research, Chandigarh, UT, India.
Introduction:FoxP3 has been well established as an indispensable protein that is required in the development and function of regulatory T cells. On the other hand,…
2018 American Transplant Congress
RNA-Seq Based Analysis of Renal Allograft Biopsies Reveals Key Mediators of Interstitial Fibrosis/Tubular Atrophy
C. McEvoy,^1,2,4 E. Brennan,^1,2 A. Dorman,^2,3 D. Sadlier,^1,2 A. Konvalinka,⁴ P. Conlon,^2,3 C. Godson.^1,2
¹Diabetes Complications Research Centre, Conway Institute, School of Medicine, University College Dublin, Dublin, Ireland; ²North Dublin Renal Biobank, Dublin, Ireland; ³Beaumont Hospital & RCSI, Dublin, Ireland; ⁴University Health Network, Toronto, Canada.
Interstitial Fibrosis/Tubular Atrophy(IFTA) is the final common pathway of most progressive renal diseases and is a potent predictors of renal outcome in both native and…
2018 American Transplant Congress
Tacrolimus Exposure in Human Proximal Tubule Cells Results in Differentially Increased CTGF Expression in Relation to Pharmacogenetic Variants of CYP3A5 and ABCB1
N. Knops,¹ Y. Ramazani,² B. Van den Heuvel,² R. Goldschmeding,³ T. Nguyen,³ E. Levtchenko,¹ D. Kuypers.⁴
¹Paediatric Nephrology and Solid Organ Transplantation, University Hospitals Leuven, Leuven, Belgium; ²Laboratory of Paediatrics, KU Leuven, Leuven, Belgium; ³Pathology, University Medical Center Utrecht, Utrecht, Netherlands; ⁴Nephrology, University Hospitals Leuven, Leuven, Belgium.
AIM: Clinical studies have demonstrated the importance of genetic variation for tacrolimus disposition and suggested a role in the development of renal fibrosis associated with…
2018 American Transplant Congress
Highly Correlative Whole-Tissue Gene/protein Signatures to Score FFPE Transplant Biopsies with Inflammation and Acute Rejection
T. Sigdel, M. Nguyen, D. Dobi, S-.C. Hsieh, J. Liberto, F. Vincenti, Z. Laszik, M. Sarwal.
Unversity of California San Francisco, San Francisco.
Background: We have previously reported the quantification of a common immune response module (CRM) of 11 genes to identify and score acute rejection (AR) across…
2018 American Transplant Congress
Chronic Antibody Mediated Rejection in Renal Allografts Shows RNA Transcript Patterns That Evolve with Stage
A. Rosales,¹ B. Adam,² M. Mengel,² T. Oura,¹ M. Matsunami,¹ T. Kawai,¹ A. Cosimi,¹ R. Smith,¹ R. Colvin.¹
¹Massachusetts General Hospital, Boston, MA; ²University of Alberta, Edmonton, AB, Canada.
Purpose. Four stages of chronic antibody mediated rejection (CAMR) in renal allografts have been defined in humans and non-human primates (NHP), beginning with donor specific…
2018 American Transplant Congress
Molecular Classifiers Can Outperform the Flawed Histologic “Gold Standard” on Which They Are Trained
J. Reeve, P. Halloran.
University of Alberta, Edmonton, Canada.
The histologic diagnosis of rejection in organ transplants is known to be an imperfect gold standard. Therefore, it might be assumed that molecular classifiers built…
2018 American Transplant Congress
Defective Innate Immune Response to Opportunistic Pathogens in Cytomegalovirus-Infected Monocytes
P. Sen, A. R. Wilkie, R. Fernandes, R. J. Lane, S. E. Hickman, T. K. Means, E. R. Rosenberg, D. M. Coen, J. A. Fishman, J. El Khoury.
Transplant and Immunocompromised Group, Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA; Department of Biological Chemistry and Pharmacology, Harvard Medical School, Boston, MA.
Human cytomegalovirus (CMV) is the most common infection encountered in transplantation and is an important cause of morbidity and mortality in both solid organ and…
2018 American Transplant Congress
Molecular Analysis of Acute Cellular Rejection in Clinical Face Transplants
T. Win, B. Dyring-Andersen, J. Teague, N. Murakami, A. Chandraker, B. Pomahac, L. Riella, R. Clark.
Brigham and Women's Hospital, Boston.
Although monitoring and immunosuppression of vascularized composite allografts (VCA) draw experience from solid organ transplantation, VCAs pose unique challenges. A deeper understanding of mechanisms underlying…
2018 American Transplant Congress
Whole-Transcriptome Sequencing to Identify Macaque Immune Gene Variants
A. Haj,¹ J. Karl,¹ R. Wiseman,¹ D. O'Connor.^1,2
¹Department of Pathology and Laboratory Medicine, University of Wisconsin, Madison, WI; ²Wisconsin National Primate Research Center, Madison, WI.
Macaque monkeys are critical in transplantation studies, yet macaque genomics lags significantly behind human genomics. While reliable genotyping methods have been developed for some macaque…