Articles tagged "Gene expression"

2022 American Transplant Congress
Characterization Of Gzmb+ Bregs Using Single Cell Rnaseq
N. Sailliet, H. Le Mai, A. Dupuy, C. Fourgeux, M. Chesneau, J. Poschmann, R. Danger, S. Brouard
CRT2I INSERM UMR1064, Nantes, France
*Purpose: Granzyme B (GZMB)+ B cells are increased in blood from transplanted patients who tolerate a kidney graft without immunosuppressive treatment. We demonstrated that these…
2022 American Transplant Congress
The Role of Gut Microbiota in Tacrolimus Blood Level Fluctuation, Time in Therapeutic Range and AlloSure Donor Derived Cell-Free DNA, Controlling for CYP3A5
M. Nayebpour¹, N. Koizumi¹, J. Melancon², M. Hasan²
¹George Mason University, Arlington, VA, ²George Washington University Hospital, Washington, DC
*Purpose: High tacrolimus variability (TV), low time in therapeutic range (TTR) and high percentage of donor derived cell-free DNA (dd-cfDNA) are associated with acute kidney…
2022 American Transplant Congress
Early Experience with OmniGraf® Biomarker Surveillance in Kidney Transplant Patients in the First-Year Post-Transplant and Beyond
V. Paramasivam¹, W. Ally², B. Sivakumar³, B. Greco¹, M. Germain¹
¹Baystate Medical Center, Springfield, MA, ²Eurofins Transplant Genomics, Framingham, MA, ³Dartmouth College, Hanover, NH
*Purpose: Non-invasive rejection biomarkers continue to evolve with recent data supporting the use of combined gene expression and donor derived cell-free DNA (dd-cfDNA) in kidney…
2022 American Transplant Congress
Characterization of Kidney Allograft Pathology Using the Histomap Gene Expression Profile (GEP) and Formalin Fixed Paraffin Embedded (FFPE) Tissue
N. Garg¹, K. Degner², H. Zhang³, W. Tian³, F. Aziz⁴, S. Parajuli², W. Zhong², N. Wilson², S. Panzer¹, A. Djamali²
¹University of Wisconsin Madison, Madison, WI, ²University of Wisconsin, Madison, WI, ³CareDx, Brisbane, CA, ⁴University of Wsiconsin, Madison, WI
*Purpose: The new Banff Human Organ Transplant (B-HOT) gene panel assessed on nCounter system has been created through a collaboration between NanoString and the Banff…
2022 American Transplant Congress
CD31⁺ Extracellular Vesicles in Ex Vivo Lung Perfusion Are Associated with Unsuitability for Transplant
G. C. Madu¹, S. Moshkelgosha², A. Chruscinski³, A. Sage², M. Cypel², S. Keshavjee², S. Juvet²
¹Temerty Faculty of Medicine, University of Toronto, Toronto, ON, Canada, ²University Health Network, Toronto, ON, Canada, ³Multi-Organ Transplantation, University Health Network, Toronto, ON, Canada
*Purpose: Primary graft dysfunction (PGD) is an important cause of morbidity and mortality following lung transplantation and results in part from injuries sustained in the…
2022 American Transplant Congress
Novel Peripheral Molecular Markers Predict Premature Graft Loss: Lessons Learned from Operational Tolerance Assessment
O. Viklicky¹, A. Le², J. Klema², E. Girmanova³, P. Mrazova³, A. Massart⁴, D. Abramowitz⁴, M. Abramowicz⁴, P. Hruba³
¹Department of Nephrology, Institute for Clinical and Experimental Medicine, Prague, Czech Republic, ²Department of Computer Science, Faculty of Electrical Engineering, Czech Technical University, Prague, Czech Republic, ³Transplant Laboratory, Institute for Clinical and Experimental Medicine, Prague, Czech Republic, ⁴Universite Libre de Bruxelles (ULB), Brussel, Belgium
*Purpose: TOMOGRAM, European multicenter study, recently identified new cohort of kidney transplant recipients (KTR) with operational tolerance (OT) with the aim to identify new transcriptomic…
2022 American Transplant Congress
LGALS1 is Expressed in Glomerular Endothelial Cells and Induced Upon Stimulation with Anti-HLA Antibody
A. Boshart¹, S. Farkona¹, S. Clotet-Freixas¹, C. McEvoy¹, J. Murphy¹, S. Crome², A. Konvalinka³
¹Toronto General Hospital Research Institute, Ajmera Transplant Centre, University Health Network, Toronto, ON, Canada, ²Immunology, Toronto General Hospital Research Institute, Ajmera Transplant Centre, University of Toronto, University Health Network, Toronto, ON, Canada, ³Nephrology, Toronto General Hospital Research Institute, Ajmera Transplant Centre, University Health Network, Toronto, ON, Canada
*Purpose: Antibody-mediated rejection (AMR) accounts for >50% of premature kidney graft loss. AMR is caused by donor specific antibodies (DSA) against human leukocyte antigens (HLA)…
2022 American Transplant Congress
Gene Profiling to Distinguish Mechanisms of Liver Allograft Rejection and Acceptance in Cynomolgus Monkey
H. Sakai¹, E. Berglund¹, S. Chaudhry¹, Y. Kato¹, E. Duggan¹, J. Weiner¹, P. Alonso-Guallart¹, N. Llore¹, K. Brustle¹, J. Stern¹, B. Piegari¹, D. Ekanayake-Alper¹, M. Martinez², G. Pierre¹, M. Danton¹, S. Kofman¹, D. Ordanes¹, D. Hajosi¹, J. Lefkowitch³, A. Iuga¹, T. Kato⁴, A. Griesemer¹
¹Columbia Center for Translational Immunology, Columbia University, New York, NY, ²Department of Pediatrics, Columbia University, New York, NY, ³Departments of Pathology and Cell Biology, Columbia University, New York, NY, ⁴Department of Surgery, Columbia University, New York, NY
*Purpose: Liver is an immune regulatory organ. Spontaneous resolutions from rejection have been seen in our orthotopic cynomolgus monkey liver transplant models for tolerance induction…
2022 American Transplant Congress
Immune Cell Phenotype of Acute Rejection of Pancreas Allografts by RNA Sequencing Analysis
C. Hughes, H. Yang, C. Snopkowski, C. Li, D. M. Dadhania, S. Sultan, C. Hartono, M. Suthanthiran, S. V. Seshan, T. Muthukumar
Weill Cornell Medicine, New York, NY
*Purpose: Long term survival of pancreas allografts are inferior to kidney allografts. Molecular mechanisms underpinning pancreas allograft rejection and failure have not been explored in…
2022 American Transplant Congress
PCR-Enhanced Performance Characteristics of TruGraf Gene Expression Profile in Kidney Transplant
P. West-Thielke¹, J. Fleming¹, C. Miller¹, N. Grund¹, A. Sutton¹, R. Sinha², M. Miller², S. Kleiboeker², J. Weems³
¹Transplant Genomics, Inc., Framingham, MA, ²Eurofins-Viracor, Lees Summit, MO, ³Transplant Genomics, Inc., Lees Summit, MO
*Purpose: The aim of this study was to perform clinical validation of TruGraf GEP using PCR methodology, as compared to the previously used microarray methodology,…