Articles tagged "Gene expression"

2019 American Transplant Congress
Pluripotent Stem Cell Differentiation: Delaying Forward Differentiation Signals Maintains Definitive Endoderm Phenotype for Cellular Transplantation
J. Fair¹, M. M. Shoja¹, N. Liu¹, I. Patrikeev², M. Motamedi², W. S. Fagg¹
¹Surgery, University of Texas Medical Branch, Galveston, TX, ²Opthalmology and Visual Sciences, University of Texas Medical Branch, Galveston, TX
*Purpose: Endodermal precursors (EP), derived in vitro from mouse pluripotent stem cell (PSC) sources, are capable of clinically useful engraftment in the unmodified (quiescent) liver…
2019 American Transplant Congress
Single Cell Sequencing of Human Kidney Allograft
H. Yang¹, H. Suryawanshi², M. Lagman¹, M. Lubetzky¹, A. Alonso¹, S. Seshan¹, T. Tuschl², M. Suthanthiran¹, T. Muthukumar¹
¹Weill Cornell Medicine, New York, NY, ²Rockefeller University, New York, NY
*Purpose: Transcriptome-based clustering of individual cells within the kidney allograft may help identify cell type-specific injury and has the potential to redefine the current histopathology-based…
2019 American Transplant Congress
Pre-Transplant Transcriptomic Signature in Peripheral Blood Predicts Early Acute Rejection after Kidney Transplant
W. Zhang¹, Z. Yi¹, C. Wei¹, K. Keung², Z. Sun¹, C. Xi¹, C. Woytovich¹, S. Farouk¹, L. Gallon³, M. Menon¹, C. Magee⁴, N. Najafian⁴, M. Samaniego⁵, A. Djamali⁶, S. Alexander², I. Rosales⁷, R. Smith⁷, P. O'Connell², R. Colvin⁷, P. Cravedi¹, B. Murphy¹
¹Icahn School of Medicine at Mount Sinai, New York, NY, ²The University of Sydney, Sydney, Australia, ³Northwestern University Feinberg School of Medicine, Chicago, IL, ⁴Brigham and Women's Hospital, Boston, MA, ⁵Henry Ford Hospital, Detroit, MI, ⁶University of Wisconsin, Madison, WI, ⁷Massachusetts General Hospital, Boston, MA
*Purpose: Early development of acute rejection after kidney transplantation is associated with diminished long-term graft survival. Predicting early acute rejection (EAR) at the time of…
2019 American Transplant Congress
Transcriptional Changes in PBMC Associated with Development of Infection and Rejection in Older and Younger Kidney Transplant Recipients
J. Schaenman¹, M. Rossetti², E. Liang¹, O. E. Beaird¹, S. Bunnapradist³, E. F. Reed², S. Cole⁴
¹Division of Infectious Diseases, David Geffen School of Medicine at UCLA, Los Angeles, CA, ²Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, ³Division of Nephrology, David Geffen School of Medicine at UCLA, Los Angeles, CA, ⁴Division of Hematology-Oncology, David Geffen School of Medicine at UCLA, Los Angeles, CA
*Purpose: Changes in immune response are central to vulnerability to infection and rejection, especially for the growing numbers of older kidney transplant recipients. We sought…
2019 American Transplant Congress
Endothelial Cells Do Not Regulate Expression of CD46, CD55 and CD59 in the Presence of Anti-HLA Class I and 11 Antibodies
A. Babu¹, T. Pham², M. Hamdorf², V. Jucaud², M. Everly², S. Daga³, D. Mitchell⁴
¹Transplant Institute, Henry Ford Hospitals, Detroit, MI, ²Terasaki Research Institute, Los Angeles, CA, ³University of Warwick, Coventry, United Kingdom, ⁴Medical Sciences, University of Warwick, Coventry, United Kingdom
*Purpose: Anti-HLA antibodies, both pre-formed and de novo are associated with worse graft survival. Complement activation is an important mechanism of antibody mediated immunological injury.…
2019 American Transplant Congress
Immune Cell Types and Host Gene Signatures Associated with Epstein-Barr Virus (EBV) Post-Transplant Lymphoproliferative Disorder (PTLD)
J. Toh¹, F. Vallania¹, W. A. Haynes¹, G. Bhagat², F. E. Craig³, S. H. Swerdlow⁴, T. Tousseyn⁵, S. M. Krams¹, P. Khatri¹, O. M. Martinez¹
¹Stanford University School of Medicine, Stanford, CA, ²Columbia University Medical Center, New York, NY, ³Mayo Clinic College of Medicine, Phoenix, AZ, ⁴University of Pittsburgh School of Medicine, Pittsburg, PA, ⁵Katholieke Universiteit Leuven, Leuven, Belgium
*Purpose: PTLD is a serious post-transplant complication that encompasses a range of abnormal lymphoproliferations, including EBV+ B cell lymphomas. Although many are EBV-driven, a significant…
2019 American Transplant Congress
Outcomes and Clinical Utility of the kSORT Assay in the Prism Prospective Clinical Trial of Highly Sensitized Kidney Transplant Recipients
A. Schroeder, P. Towfighi, C. Hu, S. Hsieh, J. Liberto, I. Damm, T. Sigdel, P. Millar, M. Sarwal, F. Vincenti
Department of Surgery, UCSF, San Francisco, CA
*Purpose: kSORT, a 17 gene blood biomarker, has been retrospectively validated for detection of biopsy proven acute rejection (AR) and immune quiescence in kidney transplantation…
2019 American Transplant Congress
Non-Invasive Monitoring of the Kidney Allograft
S. Bontha¹, G. Darville¹, C. Kuscu¹, C. Kuscu², A. Bajwa¹, J. Eason³, D. Maluf⁴, V. Mas⁵
¹Surgery, UTHSC, Memphis, TN, ²Surgery, UTHSC, memphis, TN, ³Surgery, Director of Transplant Institute - UTHSC, Memphis, TN, ⁴UTHSC, Memphis, TN, ⁵Surgery, Director of Transplant Research Institute, Memphis, TN
*Purpose: A major issue in kidney transplantation is the lack of validated non-invasive biomarkers for monitoring graft function, current biochemical markers (i.e., creatinine) have poor…
2019 American Transplant Congress
Efficacious Home Processing of Urine for Messenger RNA Profiling: A Point-of-Care Approach for Molecular Monitoring of Kidney Transplant Recipients
C. Snopkowski¹, C. Li¹, S. Albakry¹, B. Botticelli¹, M. Cassidy¹, J. Lee¹, M. Lubetzky¹, D. Dadhania¹, H. Yang¹, R. Ding¹, T. Muthukumar¹, M. Suthanthiran²
¹WCM, NYC, NY, ²WCM, New York, NY
*Purpose: Urinary mRNA profiling has been validated in multi-center studies as a robust tool for monitoring kidney transplant recipients. Home processing of urine samples for…
2019 American Transplant Congress
An 8-Gene Expression Assay in Peripheral Blood for Diagnosis of Antibody-Mediated Rejection after Kidney Transplantation
E. Van Loon¹, S. Gazut², S. Yazdani¹, E. Lerut¹, H. de Loor¹, M. Coemans¹, L. Noël³, L. Thorrez¹, L. Van Lommel¹, F. Schuit¹, D. Lambrechts¹, T. Van Brussel¹, B. Sprangers¹, D. Kuypers¹, M. Essig⁴, W. Gwinner⁵, D. Anglicheau⁶, P. Marquet⁷, M. Naesens¹
¹Catholic University Leuven, Leuven, Belgium, ²CEA, Gif-sur-Yvette, France, ³Necker-Enfants Malades Institute, Paris, France, ⁴CHU Limoges, Limoges, France, ⁵Hannover Medical School, Hannover, Germany, ⁶Paris Descartes, Sorbonne Paris Cité University, Paris, France, ⁷RTRS Centaure, Necker Hospital, Paris, France
*Purpose: Antibody-mediated rejection is a leading cause of graft failure after kidney transplantation. The diagnosis of antibody-mediated rejection is made by combining assessment of circulating…