Articles tagged "Gene expression"

2019 American Transplant Congress
The Molecular Pathology of Immune Checkpoint Inhibitor-Associated Renal Allograft Rejection: Overlap with Acute Interstitial Nephritis
B. Adam¹, C. Boils², L. Bu³, K. Renaudin⁴, K. Wen¹, M. Mengel¹
¹University of Alberta, Edmonton, AB, Canada, ²Arkana Labs, Little Rock, AR, ³University of Minnesota, Minneapolis, MN, ⁴CHU Nantes, Nantes, France
*Purpose: The use of immune checkpoint inhibitors (ICIs) for various malignancies has been associated with numerous immunological adverse events, including acute interstitial nephritis (AIN) in…
2019 American Transplant Congress
Results of Litmus (nct 02541916): The Liver Immune Tolerance Bio Marker Utilization Study
A. Chruscinski¹, V. Rojas-Luengas¹, S. Moshkelgosha¹, J. Luo², H. Yowanto², Z. Galvin¹, L. Lilly¹, R. Smith¹, E. Renner¹, J. Zhang¹, D. Grant¹, O. Adeyi¹, H. Atkins³, P. Juni⁴, N. Selzner¹, S. Juvet¹, G. Levy¹
¹Multi-Organ Transplantation, University Health Network, Toronto, ON, Canada, ²Sciex, Brea, CA, ³Ottawa Hospital Research Institute, Ottawa, ON, Canada, ⁴University of Toronto, Toronto, ON, Canada
*Purpose: Previously we reported a novel biomarker gene set for the identification of tolerance in murine models of rapamycin-induced cardiac tolerance and spontaneous hepatic tolerance.…
2019 American Transplant Congress
Molecular Microscope Proves i-IFTA Lack of Specificity
A. Perkowska-Ptasinska¹, M. Ciszek², D. Deborska-Materkowska¹, A. Debska-Slizien³, P. F. Halloran⁴, M. Myslak⁵
¹Warsaw Medical Univeristy, Warsaw, Poland, ²Department of Immunology, Transplantology and Internal Diseases, Warsaw Medical Univeristy, Warsaw, Poland, ³Gdansk Medical University, Gdansk, Poland, ⁴ATAGC, University of Alberta, Edmonton AB, AB, Canada, ⁵Pomeranian Medical University, Szczecin, Poland
*Purpose: According to 2017 Banff classification, inflammatory infiltrate in the fibrotic renal tissue with at least moderate total inflammations score (ti≥2), and tubulitis (t≥2) should…
2019 American Transplant Congress
Validation of Gene Expression Signatures Associated with Antibody-Mediated Rejection (ABMR) in Renal Allograft Biopsies (Bx) of HLA-Sensitized Patients
H. Zhang¹, C. C. Nast², N. Ammerman³, A. A. Vo³, S. C. Jordan³, M. Toyoda¹
¹Transplant Immunology Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA, ²Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA, ³Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles, CA
*Purpose: "Thoroughly validated gene transcripts” in renal Bx was adopted as one of the criteria for ABMR diagnosis in the latest Banff classification. Previously, we…
2019 American Transplant Congress
Role of Molecular Microscope in the Interpretation of Endarteritis and Acute Thrombotic Microangiopathy
M. Ciszek¹, A. Perkowska-Ptasińska¹, D. Deborska-Materkowska¹, A. Debska-Slizien², P. F. Halloran³, M. Myslak⁴
¹Warsaw Medical Univeristy, Warsaw, Poland, ²Gdansk Medical University, Gdansk, Poland, ³ATAGC, University of Alberta, Edmonton AB, AB, Canada, ⁴Pomeranian Medical University, Szczecin, Poland
*Purpose: According to the Banff classification, endarteritis (Figure 1A, case 1) may be a manifestation of either: TCMR and ABMR. Acute thrombotic microangiopathy (TMA) constitutes…
2019 American Transplant Congress
Decreased Cancer Susceptibility in Tolerant Liver Transplant Recipients: Another Benefit of Tolerance?
M. P. Bhat¹, E. Pasini¹, C. Baciu¹, S. M. Kurian², J. Levitsky³
¹Multi-Organ Transplant, UHN, Toronto, ON, Canada, ²Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA, ³Division of Gastroenterology & Hepatology, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL
*Purpose: Long-term survival of transplant recipients is significantly impacted by malignancy. Induction of tolerance is feasible in a subset of liver transplant (LT) recipients. The…
2019 American Transplant Congress
Single Cell Analysis of Kidney Allograft Rejection
C. M. Castro-Rojas¹, K. M. Roskin², C. G. Moore², B. Abu Jawdeh³, N. Salomonis², R. R. Alloway⁴, E. Woodle⁴, D. A. Hildeman¹
¹Immunobiology, Cincinnati Children's Hospital, Cincinnati, OH, ²Biomedical Informatics, Cincinnati Children's Hospital, Cincinnati, OH, ³Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, OH, ⁴Transplantation, University of Cincinnati College of Medicine, Cincinnati, OH
*Purpose: Rejection therapies (corticosteroids and antilymphocyte globulins) have not changed in over 50 years. In part this is due to a knowledge deficit of signaling…
2019 American Transplant Congress
Molecular Phenotype of Proliferative Arteriopathy in Kidney Grafts
A. Perkowska-Ptasinska¹, M. Ciszek¹, D. Deborska-Materkowska¹, A. Debska-Slizien², P. F. Halloran³, M. Myslak⁴
¹Warsaw Medical University, Warsaw, Poland, ²Gdansk Medical University, Gdansk, Poland, ³University of Alberta, Edmonton AB, AB, Canada, ⁴Pomeranian Medical University, Szczecin, Poland
*Purpose: Proliferative arteriopathy (PA) is a lesion characterized by a fibrotic tissue in the arterial intima. The phenomenon is marked by the lack of elastic…
2019 American Transplant Congress
Correlation between Gene Expression and Histology in Transbronchial Lung Transplant Biopsies
B. Adam, K. Du, S. Rotich, M. Mengel
University of Alberta, Edmonton, AB, Canada
*Purpose: Transbronchial lung transplant biopsies (TBBx) are limited by non-specific histologic lesions and suboptimal interobserver reproducibility. Gene expression offers a potentially more precise, objective and…
2019 American Transplant Congress
The Role of CD47/SIRPα and CD47/TSP-1 Pathways in Pig-to-Baboon Kidney Transplantation Using hCD47 Transgenic Kidneys
K. Takeuchi¹, Y. Ariyoshi¹, T. Pomposelli¹, L. Boyd¹, D. Ekanayake-Alper¹, S. Arn¹, R. Hawley¹, D. Ayares², M. Lorber³, M. Sykes¹, D. Sachs¹, R. Johnson⁴, K. Yamada¹
¹CCTI/Surgery, Columbia University Medical Center, New York, NY, ²Revivicor Inc., Blacksburg, VA, ³Lung BioTechnology PBC, Silver Spring, MD, ⁴Division of Renal Diseases and Hypertension, University of Colorado, Aurora, CO
*Purpose: We have recently reported that both CD80 upregulation and the degradation of SMPDL-3b on glomerular podocytes are involved in the development of post kidney…