ATC Abstracts

American Transplant Congress abstracts

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Articles tagged "FACS analysis"

  • 2019 American Transplant Congress

    Dynamic Characterization of Cells in the Perfusate from Cold Ischemic Liver Grafts

    R. J. de Vries, C. Pendexter, S. E. Cronin, B. Marques, J. F. Markmann, S. L. Stott, H. Yeh, M. Toner, K. Uygun, S. N. Tessier

    Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA

    *Purpose: Injury during preservation is a complex process that affects graft function. A deeper understanding and better prediction of liver viability are demanded to improve…
  • 2019 American Transplant Congress

    Novel Flow-Based Assay to Identify Allo-Specific T Cell Exhaustion

    N. Tanimine1, C. Rickert1, K. Lee1, K. Deng1, N. Feeney1, C. Leguern1, B. Burrell2, J. Markmann1

    1Department of Surgery, Center for Transplantation Sciences, Massachusetts General Hospital, Boston, MA, 2Immune Tolerance Network, Bethesda, MD

    *Purpose: A recent, multi-center immunosuppression withdrawal trial in liver transplant recipients unexpectedly revealed time post-transplant as an important predictor for operational tolerance. We hypothesize that…
  • 2019 American Transplant Congress

    Extracorporeal Photopheresis for Specific Immune Tolerance Responses in Heart Transplant Recipients

    M. J. Barten1, K. Klaeske2, J. Wittke2, M. Borger2, J. Garbade2, S. Lehmann3, H. Reichenspurner1, M. Dieterlen2

    1Cardiovascular Surgery, University Heart Center, Hamburg, Germany, 2Cardiac Surgery, University Heart Center Leipzig, Leipzig, Germany, 3Cardiacr Surgery, University Heart Center Leipzig, Leipzig, Germany

    *Purpose: Extracorporeal photopheresis (ECP) treatment following heart transplantation (HTx) may induce transplant tolerance. A monitoring tool for successful ECP treatment would allow determination of patient-specific…
  • 2019 American Transplant Congress

    Effects of Thalidomide and Dexamethasone Co-Treatment on CD8+ T Cells

    J. Kim1, E. Kim2, Y. Cho1, H. Lee1, M. Kim3, Y. Kim3, K. Huh3, B. Kim4

    1The Research Institute for Transplantation, Yonsei University College of Medicine, Seoul, Korea, Republic of, 2Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea, Republic of, 3Department of Transplantation Surgery, Yonsei University College of Medicine, Seoul, Korea, Republic of, 4Nephrology Division, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea, Republic of

    *Purpose: Thalidomide (TM) is known to have anti-cancer and anti-inflammatory effects and dexamethasone (DX) has been reported that it also reduces inflammation and inhibits inflammatory…
  • 2019 American Transplant Congress

    Examination of a Novel Immunoregulatory Marker in Kidney and Lung Transplant Recipients

    A. D. Desai1, G. J. Kavalam2, A. Chandraker2, S. Tripathi2

    11Harvard Summer Research Program for Kidney Medicine, Harvard Medical School,, Boston, MA, 2Schuster Family Transplantation Research Center, Brigham and Women's Hospital, Harvard Medical School, Boston, MA

    *Purpose: The T-cell immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domain (TIGIT) is a co-inhibitory receptor mainly expressed on T cells and NK cells. TIGIT has…
  • 2018 American Transplant Congress

    Immune Modulatory Effect of Thalidomide and Dexamethasone Co-Treatment on Murine Splenocytes

    E. Kim,1,2 J. Kim,1 K. Huh,1,3 Y. Cho,1 M. Kim,1,3 Y. Kim,1,3 B. Kim.1,4

    1The Research Institute for Transplantation, Yonsei University College of Medicine, Seoul, Korea; 2Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul, Korea; 3Department of Transplantation Surgery, Yonsei University College of Medicine, Seoul, Korea; 4Department of Internal Medicine, Nephrology, Yonsei University College of Medicine, Seoul, Korea.

    Thalidomide (TM) is known to have anti-inflammatory effects and dexamethasone (DX) is also well known to reduce inflammation and inhibit inflammatory cytokine production. Clinically, combinatorial…
  • 2018 American Transplant Congress

    Rituximab Interference in B-Cell Crossmatch: A Problem Solved

    P. Malvezzi,1 O. Senoussi,2 T. Jouve,1 C. De Palma,2 L. Rostaing,1 B. Bardy,2 D. Masson.2

    1Service de Néphrologie, Dialyse, Aphérèses et Transplantation, CHU Grenoble Alpes, Grenoble, France; 2Laboratoire d'Histocompatibilité, EFS Rhone Alpes, Grenoble, France.

    Background: Rituximab is commonly prescribed in ABO- or HLA-incompatible kidney transplantation. When used before transplant it interferes with B lymphocyte complement-dependent crossmatch (CDC CM) or…
  • 2018 American Transplant Congress

    Immune Phenotyping in a Pediatric Multicenter Transplant Study: Suitability of a Pre-Formulated Dry Antibody Panel System

    S. Urschel,1 L. Ionescu,1 T. Blydt-Hansen,3 B. Foster,5 S. Mital,2 U. Allan,2 S. Iveson,3 L. Hamiwka,4 J. Yap,1 V. Phan,5 P. Birk,6 L. West,1 M. Levings.3

    1U of Alberta, Edmonton, Canada; 2Sickkids, Toronto, Canada; 3U of BC, Vancouver, Canada; 4U of Calgary, Calgary, Canada; 5Montreal Children's, Montreal, Canada; 6U Manitoba, Winnipeg, Canada.

    Background:Flow cytometric immune-phenotyping is influenced by cryopreservation and inter-laboratory variability limiting comparability in multi center studies. The Duraclone™ system (DC) provides optimized pre-mixed dry antibody…
  • 2018 American Transplant Congress

    The Impact of Childhood Solid Organ Transplantation on B-Cell Maturation

    L. Ionescu,1 T. Blydt-Hansen,2 B. Foster,7 S. Mital,3 L. Hamiwka,5 U. Allan,3 V. Phan,4 P. Birk,6 C. Morgan,1 S. Urschel.1

    1U of Alberta, Edmonton, Canada; 2U of BC, Vancouver, Canada; 3Hospital for Sick Children, Toronto, Canada; 4U de Montreal, Montreal, Canada; 5U of Calgary, Calgary, Canada; 6U of Manitoba, Winnipeg, Canada; 7McGill University, Montreal, Canada.

    Background:The developing immune system of childhood promotes better graft acceptance and longevity but also higher risk for infections and post-transplant (Tx) lymphoproliferative disorders. This may…
  • 2018 American Transplant Congress

    In Vivo and In Vitro PPAR-y Activation Decreases M1-Macrophage Polarization and Improves Liver Ischemia Reperfusion Injury

    I. Linares,1 A. Bartczak,1 K. Farrokhi,1 D. Kollmann,1 M. Kaths,1 M. Hamar,1 P. Urbanellis,1 S. Ganesh,1 O. Adeyi,2 P. Yip,3 M. Selzner,1 N. Selzner.1

    1Multi Organ Transplant Program, Toronto General Hospital, Toronto, ON, Canada; 2Department of Pathology, Toronto General Hospital, Toronto, ON, Canada; 3Laboratory of Medicine and Pathobiology, Toronto General Hospital, Toronto, ON, Canada.

    Background:M1-Macrophage polarization has been studied as a potential target to decrease inflammatory response in sepsis and inflammation-related diseases. Its role in the setting of inflammatory…
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