ATC Abstracts

American Transplant Congress abstracts

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Articles tagged "FACS analysis"

  • 2019 American Transplant Congress

    Depletional Induction Therapy in Non-Human Primates – Preclinical Modeling of Polyclonal Depletion with Rhesus-Specific ATG

    R. Schmitz1, M. S. Mulvihill1, P. M. Schroder1, Z. Fitch1, F. Leopardi1, D. Magnani2, J. Kwun1, S. J. Knechtle1, A. D. Kirk1

    1Surgery, Duke University Medical Center, Durham, NC, 2University of Massachusetts Medical School, Boston, MA

    *Purpose: Antithymocyte globulins (ATG) have been used in transplantation since the late 1960s to prevent and treat acute allograft rejection. Over the past decade, induction…
  • 2019 American Transplant Congress

    Increasing Expression of B-cell Maturation Antigen during the Development of Recall Antibody Responses: A Prospective Pharmaceutical Target for B-cell Suppression?

    G. Wu, I. Kim, N. Chai, A. Klein, S. Jordan

    Cedars-Sinai Med Ctr, Los Angeles, CA

    *Purpose: Our previous study found that B cell maturation antigen (BCMA) is increasingly expressed by B/plasma cells during the development of de novo alloantibody responses.…
  • 2019 American Transplant Congress

    BCMA Expression by Alloreactive B Cells Increases during De Novo Alloantibody Responses

    N. Chai, G. Wu, I. Kim, A. Klein, S. Jordan

    Comprehensive Transplant Center, Cedars-Sinai Med Ctr, Los Angeles, CA

    *Purpose: BCMA(TNFRSF17) belongs to tumor necrosis factor receptor superfamily. It is selectively expressed during B/plasma cell differentiation while being nearly absent on naive and memory…
  • 2019 American Transplant Congress

    B Cell Derived Exosomes Are Associated with Antibody Mediated Rejection in Kidney Transplant Recipients Desensitized for a Positive Cross-Match

    D. Cucchiari1, V. Tubita2, M. J. Ramirez-Bajo2, J. Rovira2, F. E. Borràs3, L. Carreras-Planella3, A. Hertig4, F. Oppenheimer1, F. Diekmann1, I. Revuelta1

    1Renal Transplant Unit, Hospital Clínic, Barcelona, Spain, 2Laboratori Experimental de Nefrologia i Trasplantament, IDIBAPS, Barcelona, Spain, 3REMAR-IVECAT group, IGTP, Badalona, Spain, 4Department of Nephrology and Kidney Transplantation, Hôpital Tenon, Paris, France

    *Purpose: Kidney transplant recipients undergoing desensitization for a positive cross-match are at very high risk of developing Antibody-Mediated Rejection (AMR). Because of desensitization, peripheral circulating…
  • 2019 American Transplant Congress

    Dynamic Characterization of Cells in the Perfusate from Cold Ischemic Liver Grafts

    R. J. de Vries, C. Pendexter, S. E. Cronin, B. Marques, J. F. Markmann, S. L. Stott, H. Yeh, M. Toner, K. Uygun, S. N. Tessier

    Surgery, Massachusetts General Hospital and Harvard Medical School, Boston, MA

    *Purpose: Injury during preservation is a complex process that affects graft function. A deeper understanding and better prediction of liver viability are demanded to improve…
  • 2018 American Transplant Congress

    The Impact of Childhood Solid Organ Transplantation on B-Cell Maturation

    L. Ionescu,1 T. Blydt-Hansen,2 B. Foster,7 S. Mital,3 L. Hamiwka,5 U. Allan,3 V. Phan,4 P. Birk,6 C. Morgan,1 S. Urschel.1

    1U of Alberta, Edmonton, Canada; 2U of BC, Vancouver, Canada; 3Hospital for Sick Children, Toronto, Canada; 4U de Montreal, Montreal, Canada; 5U of Calgary, Calgary, Canada; 6U of Manitoba, Winnipeg, Canada; 7McGill University, Montreal, Canada.

    Background:The developing immune system of childhood promotes better graft acceptance and longevity but also higher risk for infections and post-transplant (Tx) lymphoproliferative disorders. This may…
  • 2018 American Transplant Congress

    In Vivo and In Vitro PPAR-y Activation Decreases M1-Macrophage Polarization and Improves Liver Ischemia Reperfusion Injury

    I. Linares,1 A. Bartczak,1 K. Farrokhi,1 D. Kollmann,1 M. Kaths,1 M. Hamar,1 P. Urbanellis,1 S. Ganesh,1 O. Adeyi,2 P. Yip,3 M. Selzner,1 N. Selzner.1

    1Multi Organ Transplant Program, Toronto General Hospital, Toronto, ON, Canada; 2Department of Pathology, Toronto General Hospital, Toronto, ON, Canada; 3Laboratory of Medicine and Pathobiology, Toronto General Hospital, Toronto, ON, Canada.

    Background:M1-Macrophage polarization has been studied as a potential target to decrease inflammatory response in sepsis and inflammation-related diseases. Its role in the setting of inflammatory…
  • 2018 American Transplant Congress

    Multi-Targeting Desensitization Therapy Alters Peripheral B-Cell Homeostasis and Suppresses Recall Alloantibody Responses

    I. Kim, G. Wu, N-.N. Chai, A. Klein, S. Jordan.

    CTC/Surgery, Cedars-Sinai Medical Center, Los Angeles, CA.

    Purpose: Occurring of donor specific antibodies (DSA) is injurious to organ transplant. Development of immunotherapies to suppress DSA, therefore, has been one of the strategic…
  • 2018 American Transplant Congress

    Analysis of Circulating CD38+/CD138+ Plasma Cells during Development of De Novo Donor Specific Antibody Responses

    N-.N. Chai, G. Wu, I. Kim, S. Jordan, A. Klein.

    CTC/Surgery, Cedars-Sinai Medical Center, Los Angeles, CA.

    Purpose: Donor specific antibodies (DSA) that emerge following transplantation are indicative of allogenic sensitization and a predisposing factor for antibody mediated rejection. Though various methods…
  • 2018 American Transplant Congress

    Immune Modulatory Effect of Thalidomide and Dexamethasone Co-Treatment on Murine Splenocytes

    E. Kim,1,2 J. Kim,1 K. Huh,1,3 Y. Cho,1 M. Kim,1,3 Y. Kim,1,3 B. Kim.1,4

    1The Research Institute for Transplantation, Yonsei University College of Medicine, Seoul, Korea; 2Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul, Korea; 3Department of Transplantation Surgery, Yonsei University College of Medicine, Seoul, Korea; 4Department of Internal Medicine, Nephrology, Yonsei University College of Medicine, Seoul, Korea.

    Thalidomide (TM) is known to have anti-inflammatory effects and dexamethasone (DX) is also well known to reduce inflammation and inhibit inflammatory cytokine production. Clinically, combinatorial…
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